Loss-of-Function of a Tomato Receptor-Like Kinase Impairs Male Fertility and Induces Parthenocarpic Fruit Set

Takei, Hitomi; Shinozaki, Yoshihito; Yano, Ryoichi; Kashojiya, Sachiko; Hernould, Michel; Chevalier, Christian; Ezura, Hiroshi; Ariizumi, Tohru

doi:10.3389/fpls.2019.00403

Cited by 15 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From tomato mutant lines, several mutations, which cause phenotypes, have been identified. The lossof-function of receptor-like kinase in tomato induces parthenocarpic fruit set, with impairment of male fertility (Takei et al 2019). Disruption of the tomato HAWAIIAN SKIRT ortholog results in facultative parthenocarpy (Damayanti et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Genome editing in PDS genes of tomatoes by non-selection method and of Nicotiana benthamiana by one single guide RNA to edit two orthologs

Komatsu

Abdellatif

Yuan

et al. 2020

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

The CRISPR/Cas9 system is widely used for targeted mutagenesis in many organisms including plants. For application of this system, tissue culture methods need to be established. In this study, detailed methods for introduction of mutations in tomato and Nicotiana benthamiana plants using the CRISPR/Cas9 system are described. The methods include tissue culture protocols for tomato and N. benthamiana. We also demonstrate the methodology to generate Cas9free genome edited tomato plants and use of one single guide RNA (sgRNA) to edit two orthologs in N. benthamiana. The examples of editing the PHYTOENE DESATURASE (PDS) genes in these plants are also provided. The Cas9-free tomato line was obtained when tomato plants were cultured on a non-selective medium after transformation with the CRISPR/Cas9 system. Two orthologs of PDS in N. benthamiana were mutated using a sgRNA, because these orthologs contain the same nucleotide sequences with PAM motif. These mutations were inherited to the next generation. The mutations in the PDS genes resulted in an albino phenotype in tomato and N. benthamiana plants. These results demonstrate that the non-selective method is one of the ways to obtain Cas9-free genome editing in tomato plants and that the two orthologs can be edited by one sgRNA in N. benthamiana.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome editing in PDS genes of tomatoes by non-selection method and of Nicotiana benthamiana by one single guide RNA to edit two orthologs

Komatsu

Abdellatif

Yuan

et al. 2020

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…An additional or alternative explanation for this parthenocarpic trait is that the loss of stamens, as a consequence of suppression of the class B MdPI , results in male sterility. Male sterility has been associated with parthenocarpic fruit production in multiple plant species, including tomato ( Ingrosso et al, 2011 ; Medina et al, 2013 ; Takei et al, 2019 ), citrus ( Vardi et al, 2008 ), and grape ( Royo et al, 2016 ). In contrast, ectopic MdPI expression coverts sepals to petals and inhibits apple fruit growth toward the base of the fruit resulting in short and flat fruit ( Figure 3 ).…”

Section: Pi/ap3 Homologs Negatively Regulate Fruit Growthmentioning

confidence: 99%

The Roles of Floral Organ Genes in Regulating Rosaceae Fruit Development

Yao

Kang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The function of floral organ identity genes, APETALA1/2/3, PISTILLATA, AGAMOUS, and SEPALLATA1/2/3, in flower development is highly conserved across angiosperms. Emerging evidence shows that these genes also play important roles in the development of the fruit that originates from floral organs following pollination and fertilization. However, their roles in fruit development may vary significantly between species depending on the floral organ types contributing to the fruit tissues. Fruits of the Rosaceae family develop from different floral organ types depending on the species, for example, peach fruit flesh develops from carpellary tissues, whereas apple and strawberry fruit flesh develop from extra-carpellary tissues, the hypanthium and receptacle, respectively. In this review, we summarize recent advances in understanding floral organ gene function in Rosaceae fruit development and analyze the similarities and diversities within this family as well as between Rosaceae and the model plant species Arabidopsis and tomato. We conclude by suggesting future research opportunities using genomics resources to rapidly dissect gene function in this family of perennial plants.

show abstract

“…The silencing of these genes by RNA interference resulted in parthenocarpic fruit development in tomato with some other auxin-related phenotypes [137]. Takei et al [138] isolated and characterized small parthenocarpic fruit and flower (spff) mutants in a tomato cultivar. Linkage analysis and RNAi-based silencing of the Solyco4g077010 gene, which encodes the receptor-like protein kinase, resulted in impaired male sterility with parthenocarpic fruit set development.…”

Section: Seedless Fruit Developmentmentioning

confidence: 99%

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Rajput

Choudhary

Kumar

et al. 2021

Plants

View full text Add to dashboard Cite

With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA-based approaches i.e., RNAi-mediated gene regulation and the site-specific nuclease-based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting the downregulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops’ genome sequences to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture.

show abstract

Loss-of-Function of a Tomato Receptor-Like Kinase Impairs Male Fertility and Induces Parthenocarpic Fruit Set

Cited by 15 publications

References 55 publications

Genome editing in <i>PDS</i> genes of tomatoes by non-selection method and of <i>Nicotiana benthamiana</i> by one single guide RNA to edit two orthologs

Genome editing in <i>PDS</i> genes of tomatoes by non-selection method and of <i>Nicotiana benthamiana</i> by one single guide RNA to edit two orthologs

The Roles of Floral Organ Genes in Regulating Rosaceae Fruit Development

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Contact Info

Product

Resources

About