Loss‐of‐function mutations in <i>DET2</i> gene lead to an enhanced resistance to oxidative stress in <i>Arabidopsis</i>

Cao, Shuqing; Xu, Quanhua; Cao, Yajun; Qian, Kun; An, Kun; Zhu, Yong; Binzeng, Hu; Zhao, Huifang; Kuai, Benke

doi:10.1111/j.1399-3054.2004.00432.x

Cited by 171 publications

(84 citation statements)

References 55 publications

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“…However, it is difficult to analyze genetically the role and action mechanisms of BR in plant stress tolerance because of the strong and pleiotropic phenotypes of BR biosynthesis and signaling mutants, including extreme dwarfism, dark green and epinastic leaves, and delayed development (Khripach et al, 2000;Bishop and Koncz 2002;Mü ssig et al, 2002;Cao et al, 2005). Moreover, current techniques allow for measurement of stress-induced changes in the levels of only intermediates in the BR biosynthesis pathway but not of the bioactive brassinolide (Nakashita et al, 2003;Jager et al, 2008).…”

Section: Discussion Br Induces Stress Tolerance In Cucumbermentioning

confidence: 99%

Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber

et al. 2009

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Brassinosteroids (BRs) induce plant tolerance to a wide spectrum of stresses. To study how BR induces stress tolerance, we manipulated the BR levels in cucumber (Cucumis sativus) through a chemical genetics approach and found that BR levels were positively correlated with the tolerance to photo-oxidative and cold stresses and resistance to Cucumber mosaic virus. We also showed that BR treatment enhanced NADPH oxidase activity and elevated H 2 O 2 levels in apoplast. H 2 O 2 levels were elevated as early as 3 h and returned to basal levels 3 d after BR treatment. BR-induced H 2 O 2 accumulation was accompanied by increased tolerance to oxidative stress. Inhibition of NADPH oxidase and chemical scavenging of H 2 O 2 reduced BR-induced oxidative and cold tolerance and defense gene expression. BR treatment induced expression of both regulatory genes, such as RBOH, MAPK1, and MAPK3, and genes involved in defense and antioxidant responses. These results strongly suggest that elevated H 2 O 2 levels resulting from enhanced NADPH oxidase activity are involved in the BR-induced stress tolerance.

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Section: Discussion Br Induces Stress Tolerance In Cucumbermentioning

confidence: 99%

Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber

et al. 2009

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“…The enzymatic antioxidative system consists of several enzymes such as guaiacol peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ARORA et al ascorbate peroxidase (APX), and glutathione reductase (GR) (Asada and Takahashi, 1987;Arora et al, 2002). A number of plant hormones such as ethylene, abscisic acid, salicylic acid and steroids are involved in the regulation of the plant antioxidative enzymatic system (Cao et al, 2005).…”

Section: -Homobrassinolídeo Reduz O Estresse Oxidativo Em Plantas Dmentioning

confidence: 99%

28-Homobrassinolide alleviates oxidative stress in salt-treated maize (Zea mays L.) plants

Arora

Bhardwaj

Sharma

et al. 2008

Braz. J. Plant Physiol.

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The present investigation was undertaken to study the effects of 28-homobrassinolide on the activities of antioxidative enzymes such as superoxide dismutase (EC 1.15.1.1), guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2) and ascorbate peroxidase (EC 1.11.1.11), as well as protein and malondialdehyde concentrations in 30-d-old plants of Zea mays L. grown under salt stress. The seeds were soaked in 28-homobrassinolide solutions (0, 10 -8 , 10-6 and 10 -4 mM) for 12 h and then sown in the field in a randomized block layout. The blocks were salinised with NaCl at concentrations of 0, 25, 50 and 75 mM. The activities of antioxidative enzymes and protein concentration increased in 28-homobrassinolide-treated plants. Despite the enhancement of enzyme activities under salt stress alone, lipid peroxidation increased and protein concentration decreased. However, pre-sowing treatments of 28-homobrassinolide further enhanced the activities of antioxidative enzymes in addition to lowering lipid peroxidation and increasing protein concentration, thus suggesting that 28-homobrassinolide can alleviate oxidative stress in salt-treated maize plants.

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“…Various studies have highlighted BR induced abiotic stress tolerance in plants [19]- [21] [55] [56], however the precise mechanism of action remained enigmatic, primarily due to the strong and pleiotropic phenotypes of BR biosynthesis and signaling mutants, including extreme dwarfism, associated with dark green and epinastic leaves [55] [57]- [59]. Microarray analysis revealed BR induced expression of stress associated genes including heat shock protein and oxidative stress related genes in Arabidopsi [58] [60].…”

Section: Role Of Br In Stress Tolerancementioning

confidence: 99%

Improving Photosynthetic Responses during Recovery from Heat Treatments with Brassinosteroid and Calcium Chloride in Indian Bread Wheat Cultivars

Hairat¹,

Khurana²

2015

AJPS

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Climate change is expected to unleash severe and frequent heat waves in future, adversely affecting crop productivity. The aim of this study was to examine the effect of two separate episodes of heat stress, mimicking heat wave conditions on the physiology of four Indian bread wheat cultivars and to study the ameliorating effects of epibrassinolide (BR) and calcium chloride on the recovery of these cultivars. The two thermo-tolerant cultivars C306 and K7903 suffered less inhibition of photosystem II efficiency as compared to the two thermo-susceptible cultivars HD2329 and PBW343. Application of BR and calcium chloride resulted in faster recovery in all the four cultivars. Measurement of the minimum fluorescence (Fo) versus temperature curves revealed a higher inflection temperature of Fo (Ti) for the two tolerant cultivars as compared to the susceptible cultivars, emphasizing greater thermo stability of the photosynthetic apparatus. The two thermotolerant cultivars showed higher photochemistry (ɸPSII) relative to the two susceptible cultivars. An increase in the steady state fluorescence was observed in both the susceptible cultivars as compared to the tolerant cultivars. Expression analysis revealed faster recovery of the transcripts involved in photosynthesis in tolerant cultivars as compared to susceptible cultivars. Exogenous application of the ameliorating compounds resulted in faster recovery of transcripts in all the cultivars. The result suggested that under severe stress conditions tolerant cultivars showed faster recovery and a better thermo-stability of its photosynthetic apparatus as compared to susceptible cultivars and application of epibrassinolide and calcium chloride could ameliorate the damaging effect of severe temperature stress to a considerable level in all the four cultivars under study.

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Loss‐of‐function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis

Cited by 171 publications

References 55 publications

Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber

Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber

28-Homobrassinolide alleviates oxidative stress in salt-treated maize (Zea mays L.) plants

Improving Photosynthetic Responses during Recovery from Heat Treatments with Brassinosteroid and Calcium Chloride in Indian Bread Wheat Cultivars

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