The Jovian magnetosphere is highly dynamic, influenced by both solar wind and internal processes associated with the rapid planetary rotation and Io's volcanic activities. Accompanying the mass and energy circulations driven by the magnetospheric dynamics, the magnetic configuration also changes dramatically. One of the crucial parameters to characterize the magnetic configuration is magnetic field line curvature (FLC), which generally describes how stretched the field line is. The curvature is pivotal to influence particle behaviors, for example, pitch angle scattering which may lead to auroral particle precipitation. In this work, a method is proposed to investigate the real‐time magnetic FLC in Jovian current sheet using the magnetic field data from the Juno spacecraft. The results indicate that the FLC scattering of ions and relativistic electrons are common in Jovian magnetosphere, providing a crucial insight to understand the particle behaviors.