Loss of EGF‐dependent cell proliferation ability on radioresistant cell HepG2‐8960‐R

Saito, Yohei; Abiko, Ryo; Kishida, Akira; Kuwahara, Yoshikazu; Yamamoto, Yumi; Yamamoto, Fujio; Fukumoto, Manabu; Ohkubo, Yasuhito

doi:10.1002/cbf.3090

Cited by 5 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results from other groups confirmed that migration, invasion, proliferation and anti-apoptotic ability is changed accompanied by the acquired radioresistance (10,12,15). Different from the previous studies showing reduced proliferation ability of radioresistant cells (46,47), we found significant increase in proliferation of resistant cells compared to their parental cells. Recently, Tahmasebi-Birgani et al also observed that fractionated irradiation induces the radioresistance, along with the inhibition or enhancement of the proliferative ability, depending on the cellular context (48).…”

Section: Discussionsupporting

confidence: 70%

Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells

Feng¹,

Fan²,

Yu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels. Methods:We established an acquired radioresistant cell line from its parental NF639 cell line (HER2positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. RNA-sequencing (RNA-seq) was also used to reveal the potential regulating genes and molecular mechanisms associated with the acquired changed phenotypes. Real-time PCR was used to validate the results of RNA-seq.Results: The NF639R cells exhibited increased radioresistance and enhanced activity of proliferation, migration and anti-apoptosis, but decreased basal ROS. Two main energy metabolism pathways, mitochondrial respiration and glycolytic, were also upregulated. Furthermore, 490 differentially expressed genes were identified by RNA-seq. Enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes showed many differently expressed genes were significantly enriched in cell morphology, proliferation, migration, anti-apoptosis, antioxidation, tumor stem cells and energy metabolism and the signaling cascades such as the transforming growth factor-β, Wnt, Hedgehog, vascular endothelial growth factor, retinoic acid-inducible gene I (RIG-I)-like receptor, Toll-like receptor and nucleotide oligomerization domain (NOD)-like receptor were significantly altered in NF639R cells. Conclusions:In clinical radiotherapy, repeat radiotherapy for short-term recurrence of breast cancer may result in enhanced radioresistance and promote malignant progression. Our research provided hints to understand the tumor resistance to radiotherapy de novo and recurrence with a worse prognosis following radiotherapy.

show abstract

Section: Discussionsupporting

confidence: 70%

Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells

Feng¹,

Fan²,

Yu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…Song et al reported that miR-7-5p enhance autophagy via EGFR/AKT/mTOR signaling [ 26 ]. In CRR cells, it has been reported that the expression of the EGF receptor and phosphorylation of AKT decreased [ 20 , 27 ], and as reported by Song et al, the mTOR signaling was enhanced [ 28 ]. Conversely, it has been reported that the doxorubicin–resistant cell was down-regulated by the miR-7-5p expression [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines

Tomita

Nagasawa

Kuwahara

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

In cancer therapy, radioresistance or chemoresistance cells are major problems. We established clinically relevant radioresistant (CRR) cells that can survive over 30 days after 2 Gy/day X-ray exposures. These cells also show resistance to anticancer agents and hydrogen peroxide (H2O2). We have previously demonstrated that all the CRR cells examined had up-regulated miR-7-5p and after miR-7-5p knockdown, they lost radioresistance. However, the mechanism of losing radioresistance remains to be elucidated. Therefore, we investigated the role of miR-7-5p in radioresistance by knockdown of miR-7-5p using CRR cells. As a result, knockdown of miR-7-5p increased reactive oxygen species (ROS), mitochondrial membrane potential, and intracellular Fe2+ amount. Furthermore, miR-7-5p knockdown results in the down-regulation of the iron storage gene expression such as ferritin, up-regulation of the ferroptosis marker ALOX12 gene expression, and increases of Liperfluo amount. H2O2 treatment after ALOX12 overexpression led to the enhancement of intracellular H2O2 amount and lipid peroxidation. By contrast, miR-7-5p knockdown seemed not to be involved in COX-2 and glycolysis signaling but affected the morphology of CRR cells. These results indicate that miR-7-5p control radioresistance via ROS generation that leads to ferroptosis.

show abstract

Clinically relevant radioresistant cell line: a simple model to understand cancer radioresistance

et al. 2017

View full text Add to dashboard Cite

Radiotherapy (RT) is one of the major modalities for the treatment of human cancers and has been established as an excellent local treatment for malignant tumors. Conventional fractionated RT consists of 2-Gy X-rays, fractionated once a day, 5 days a week for 5-7 weeks in total 60 Gy. The efficacy of RT depends on the existence of radioresistant cells, which remains one of the most critical obstacles in RT and radio-chemotherapy. To improve the efficacy of RT, understanding the characteristics of radioresistant cells is one of the important subjects in radiation biology. Several studies have been reported to find out molecules implicated in radioresistance. However, it is noteworthy that cellular radioresistance has been mainly studied among cells with different genetic backgrounds and different origins. Therefore, making a system to compare between radioresistant and sensitive cells with the isogenic background is required. In this review, some aspects of cellular radioresistance mainly focusing on clinically relevant radioresistant (CRR) cell lines that can continue to proliferate even under exposure to 2-Gy X-rays, once a day, for more than 30 days, which is consistent with the conventional fractionated RT are discussed.

show abstract

Loss of EGF‐dependent cell proliferation ability on radioresistant cell HepG2‐8960‐R

Cited by 5 publications

References 30 publications

Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells

Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells

MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines

Clinically relevant radioresistant cell line: a simple model to understand cancer radioresistance

Contact Info

Product

Resources

About