Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium

Ardjomand, Navid; Berghold, Andrea; Reich, M. E.

doi:10.1038/eye.1998.22

Cited by 41 publications

(32 citation statements)

References 16 publications

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“…On the other hand, it has been found that during organ culture the number of APCs in the corneoscleral discs slowly decreases. 9-13 Pels et al 9 and Ardjomand et al 10,11 looked only for HLA-DR positivity in their analyses and found a complete vanishing (Pels et al 9 ) or a significant reduction (Ardjomand et al 10,11 ) of HLA-DR-positive DCs within 7 days of organ culture, which was independent of the storage media. More recently Al-Fakih et al 12 also looked for HLA-DR-positive cells in corneoscleral discs and found that the decrease of these cells was faster when corneas were stored in organ culture compared with hypothermic storage.…”

Section: Discussionmentioning

confidence: 97%

“…These APCs have the capacity to determine the outcome of immunity or tolerance within the anterior ocular segment. 8 It has been shown that the number of LCs and DCs slowly decreases during organ culture, [9][10][11][12][13] although it seems that they do not vanish completely. Mayer et al 13 found that human corneas harbour different populations of LCs and DCs in a distribution pattern similar to that in the skin.…”

Section: Introductionmentioning

confidence: 99%

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Prolonged organ culture reduces the incidence of endothelial immune reactions

Maier¹,

Heinzelmann²,

Böhringer³

et al. 2015

Eye

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Purpose The number of antigen-presenting cells decreases during organ culture of corneoscleral discs. This might result in a decrease of immune reactions with increasing duration of organ culture. To investigate this hypothesis, we performed a retrospective analysis of all penetrating keratoplasties that were consecutively performed over the last 5 years. Patients and methods All cases of penetrating keratoplasties (n = 1006) were divided into two groups, with the division made at the median of the storage time (21 days). These two groups were compared by a Cox proportional hazards survival model regarding the incidence of endothelial immune reactions, clear graft survival, and chronic endothelial cell loss following penetrating keratoplasty considering patient's age, donor's age, and risk situation as co-variates. Results We observed statistically significantly fewer endothelial immune reactions (20.1% (95% confidence interval 15.5-24.5%) after 2 years) in the group with a storage time of more than 21 days compared with the group with a storage time of o21 days (26.5% (95% confidence interval 21.6-31.2%) after 2 years). However, the duration of organ culture did not have a statistically significant effect on clear graft survival or chronic endothelial cell loss. Conclusion Our results demonstrate that an increased duration of organ culture leads to a lower incidence of endothelial immune reactions following penetrating keratoplasty. However, we do not recommend increased storage times in general as overall graft survival did not improve. The reason for this apparent paradox may be that the endothelial cell count decreases during storage time.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Prolonged organ culture reduces the incidence of endothelial immune reactions

Maier¹,

Heinzelmann²,

Böhringer³

et al. 2015

Eye

View full text Add to dashboard Cite

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“…Since immunological rejection remains one of the main causes of corneal graft rejection, and APC in the donor tissue are thought to be important contributors to rejection, reducing the number of APC during corneal storage may have consequences for corneal graft outcome [2,22] .…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the number of dendritic cells declined after 4 days of culturing, and such cells were absent after 7 days, as detected by immunofluorescent and histochemical approaches [12] . A decrease in and even a complete loss of HLA-DRpositive cells were described following 7 or 14 days of storage under hypothermic or tissue culture conditions, respectively [22] .…”

Section: Introductionmentioning

confidence: 99%

A Decrease in the Density of HLA-DR-Positive Cells Occurs Faster in Corneas Stored in Organ Culture than under Hypothermic Conditions

Al-Fakih

Faltus²,

Jirsová³

2011

Ophthalmic Res

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Aims: To compare the number of antigen-presenting cells (APC) at various locations in fresh human corneoscleral disks and in those that are stored for grafting under hypothermic conditions or in organ culture (OC) with the aim of determining the conditions under which the decline in APC numbers is most substantial. Methods: Cryosections obtained from fresh corneoscleral disks and disks stored under hypothermic (Optisol-GS) or OC conditions were used. The density of HLA-DR-positive cells was determined on cross sections using enzyme immunohistochemistry (alkaline phosphatase-antialkaline phosphatase technique). Longitudinal sections were used for detecting ATPase activity. Results: The densities of HLA-DR-positive cells in both the epithelium and stroma increased from the central (3.79 and 0.61/mm²) to the peripheral cornea (5.56 and 1.35/mm²) as well as to the limbus and conjunctiva. A marked decrease in the number of HLA-DR-positive cells occurred after 7 days of storage in all corneal areas, the limbus and conjunctiva, compared to fresh tissue. No positive cells were found in the epithelium of corneas after 14 days in OC and after 21 days in hypothermic storage. Twenty-eight days of storage in OC led to the complete absence of HLA-DR-positive cells in the epithelium of the limbus and conjunctiva, and to a significant reduction in the stroma. Conclusion: Corneas stored in OC longer than 14 days are likely to be less immunogenic than corneas stored under hypothermic conditions, thus resulting in a possible positive effect on prolonging graft survival.

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“…On contrary, a loss of Langerhans cells has been observed during storage of corneal transplants, which was independent from preservation medium (organ culture or hypothermic storage) and preservation time. 39 Therefore, these grafts' attributes should neither influence active TGF-b 2 levels in the aqueous humour nor increase the frequency of immune reactions following PK.…”

Section: Discussionmentioning

confidence: 99%

Decreased active TGF-β2 levels in the aqueous humour during immune reactions following penetrating keratoplasty

Maier¹,

Broszinski²,

Heizmann³

et al. 2007

Eye

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Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium

Abstract: These results demonstrate that loss of HLA-DR antigens is mainly related to storage period and is independent of the type of preservation medium and preservation temperature.

Cited by 41 publications

References 16 publications

Prolonged organ culture reduces the incidence of endothelial immune reactions

Prolonged organ culture reduces the incidence of endothelial immune reactions

A Decrease in the Density of HLA-DR-Positive Cells Occurs Faster in Corneas Stored in Organ Culture than under Hypothermic Conditions

Decreased active TGF-β2 levels in the aqueous humour during immune reactions following penetrating keratoplasty

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