Loss of Chromosomes and Nondisjunction induced by Caffeine in Drosophila

Mittler, Sidney; Mittler, Jeanne E.; Owens, Sally

doi:10.1038/214424a0

Cited by 25 publications

(7 citation statements)

References 7 publications

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“…A finely-drawn glass needle with a tip of S. Mittler et al about 0-06mm (Mittler and Zitnik 1963) was inserted between the fourth and fifth dorsal tergite and approximately 0 1 . A finely-drawn glass needle with a tip of S. Mittler et al about 0-06mm (Mittler and Zitnik 1963) was inserted between the fourth and fifth dorsal tergite and approximately 0 1 .…”

Section: Methodsmentioning

confidence: 99%

“…The males were transferred daily, or every 2 days, to a group of virgin females that were 2 to 3 days old. We abandoned the rod chromosome used previously (Mittler 1965) infavour ofthering X 2 yBchromosome, for although spontaneous loss of the ring chromosome is more common, there is a ten-to fifteen-fold increase in the number of XO males after irradiation of the ring chromosome. In our laboratory, where the flies were cultured at 24°c, when Drosophila males 2-16 hours old were irradiated and mated daily in the ratio of 1 male to 3 females, the first brood to show induced crossing-over was produced during the 7 to 8 day period after irradiation (Mittler 1965).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Urethane and Colchicine on Radiation-induced Chromosome Loss in MaleDrosophila

Mittler

Arnesen

Raymond

1966

International Journal of Radiation Biology and Related Studies

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Urethane or colchicine was injected, either before or immediately after 2000 r of x-rays, into male Drosophila melanogaster. Urethane modified the radiationinduced loss of the ring X or Y sc 8 y+ chromosome, as determined by the number of XO male offspring. Urethane alone increased the loss of the ring X chromosome primarily in the mature spermatozoa and older spermatids, whereas x-rays induced chromosome loss to a greater extent in those cells in or about meiosis. Pre-treatment or post-treatment with colchicine by means of injection near the testis gave an apparent protection against radiation-induced chromosome loss; but colchicine alone induced sterility by reducing the number of gametes available to form zygotes by affecting those cells in spermatogenesis that were in or about meiosis at the time of treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Urethane and Colchicine on Radiation-induced Chromosome Loss in MaleDrosophila

Mittler

Arnesen

Raymond

1966

International Journal of Radiation Biology and Related Studies

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“…6. Bands and puffs are distinct, x 320. treated males of Drosophila due to non-disjunction (Mittler, Mittler & Owens, 1967).…”

Section: Effect On Salivary Gland Chromosomesmentioning

confidence: 99%

EFFECTS OF CAFFEINE ON GROWTH AND METAMORPHOSIS OF MOTH FLY TELMATOSCOPUS ALBIPUNCTATUS (DIPTERA, PSYCHODIDAE

Sehgal

Simões

Jurand

1977

Entomologia Exp Applicata

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Caffeine causes severe retardation of growth and development and high mortality of larvae Telmatoscopus albipunctatus. In general, sensitivity to caffeine is inversely proportional to the age of the treated larvae. Adults originating from treated larvae show lower fertility. Treatment of pupae results in elongated abdomens, abnormal wings and reduced fertility in adults. Caffeine also affects adversely polytenization of chromosomes. It is suggested that effects on larval growth and polytenization of chromosomes are due to retardation of DNA synthesis. Other possible mechanisms of action of caffeine are discussed. ZUSAMMENFASSUNG WIRKUNGEN VON KOFFEIN AUF WACHSTUM UND METAMORPHOSE DER SCHMETTERLINGSMÜCKE TELMATOSCOPUS ALBIPUNCTATUS (DIPTERA, PSYCHODIDAE) Koffein verursacht starke Verzögerung von Wachstum und Entwicklung sowie hohe Mortalität der Larven von Telmatoscopus albipunctatus. Im allgemeinen ist die Empfindlichkeit gegenüber Koffein umgekehrt proportional zum Alter der behandelten Larven. Adulte, die als Larven damit behandelt wurden, zeigen verminderte Fertilität. Behandlung von Puppen ergibt Adulte mit verlängertem Abdomen, anormalen Flügeln und verminderter Fertilität. Koffein beeinflußt auch die Polytänisierung der Chromosomen ungünstig. Es wird angenommen, daß die Beeinflussung des Larvenwachstums und der Polytänisierung der Chromosomen zurückzuführen ist auf verzögerte DNS‐Synthese. Es werden sodann andere mögliche Mechanismen der Wirkung des Koffeins diskutiert.

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“…Caffeine (1,3,7-trimethylxanthine) and its derivatives have been reported to be mutagenic to bacteria (Demerec, Bertani & Flint, 1951 ; Grigg, 1968), yeast (Loprieno & Schupbach, 1971 ; Fabre, 1972), plants (Kihlman & Levan, 1949;Yamamoto & Yamaguchi, 1969), and Drosophila (Andrew, 1959;Mittler, Mittler & Owens, 1967; Kuhlmann, Fromme, Heege & Ostertag, 1968). Decreases in litter size and increases in fetal résorption resulted when caffeine was injected into pregnant rodents (Nishimura & Nakai, 1960; Gilbert & Pistey, 1973).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary caffeine on the testis of the domestic fowl, Gallus domesticus

Collier²,

Lodge

1976

Reproduction

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Roosters were fed 0-1% caffeine mixed by weight into a standard ration. With continued dietary caffeine administration, the average fertility of eggs collected for 2 weeks from untreated pullets inseminated with semen from the treated males at 0, 7 and 14 days after the start of treatment was 30-8, 33-5 and 3-3%, respectively. After 14 days of treatment fertility was significantly lower (P less than 0-001) than before (0 days) or 7 days after treatment. Semen output and sperm concentration were markedly reduced 17-21 days after treatment, and no semen could be collected from the roosters after they had received caffeine for 30 days. Removal of dietary caffeine resulted in resumption of semen production and a return of fertility to the control level. Testicular histology showed that spermatocyte divisions ceased and spermiogenesis was abnormal, although Leydig tissue and the response of the males to massage for semen collection was not affected. The effects on spermatogenesis and fertility were reversible after treatment for 30 days.

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Loss of Chromosomes and Nondisjunction induced by Caffeine in Drosophila

Cited by 25 publications

References 7 publications

Effect of Urethane and Colchicine on Radiation-induced Chromosome Loss in MaleDrosophila

Effect of Urethane and Colchicine on Radiation-induced Chromosome Loss in MaleDrosophila

EFFECTS OF CAFFEINE ON GROWTH AND METAMORPHOSIS OF MOTH FLY TELMATOSCOPUS ALBIPUNCTATUS (DIPTERA, PSYCHODIDAE

Effects of dietary caffeine on the testis of the domestic fowl, Gallus domesticus

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