Loss of adenylate cyclase activity in variants of Bordetella pertussis

Parton, R.

doi:10.1016/0378-1097(78)90108-8

Cited by 7 publications

(12 citation statements)

References 4 publications

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“…The mechanism of antigenic modulation in B. pertussis is not known, but a possible role for adenylate cyclase as a regulator of gene expression is suggested by the low activity of this enzyme in C-mode cells (Parton and Durham, 1978). However, the studies reported here indicate that not only is there a cessation of synthesis of certain virulence and other factors of B.pertussis in C-medium, but there is active destruction of these factors in the cells of the X-mode inoculum.…”

Section: Discussionmentioning

confidence: 99%

“…Lacey (1 960) introduced the term "X-mode" for cells with haemagglutinin, certain agglutinogens and mouse virulence, and "C-mode" for cells that had lost these properties as a result of growth in a medium with a high concentration of MgS04. Later studies showed that protective antigen (PA), histamine-sensitising factor (HSF), leukocytosis-promoting factor (LPF), heat-labile toxin (HLT), adenylate cyclase activity and 28k and 30k envelope polypeptides of B. pertussis were also lost in similar growth conditions (Parton and Wardlaw, 1975;Wardlaw, Parton and Hooker, 1976;Livey, Parton and Wardlaw, 1978;Parton and Durham, 1978). There was some indication from Lacey's work that the haemagglutinin was lost faster than the agglutinogens, but the sequence of loss of the other properties was not examined.…”

Section: Introductionmentioning

confidence: 99%

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Rapidity of antigenic modulation of Bordetella pertussis in modified Hornibrook medium

Idigbe

Parton

Wardlaw

1981

Journal of Medical Microbiology

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SUMMARY. Modulation of Bordetella pertussis was induced by growth in Hornibrook medium with a high content of magnesium sulphate (C-medium); four pathophysiological activities in the cells or in the whole culture were measured at intervals. Modulation, shown by the extensive loss of protective antigen, histamine-sensitising factor, leukocytosis-promoting factor, heat-labile toxin and X-mode specific envelope proteins, occurred during the first 10 h of incubation of X-mode cells in C-medium at 35°C and before the onset of the logarithmic phase of growth. The rapidity of these losses was greater than could be explained by a simple growth-dilution effect and did not appear to be due to release of activity into the culture fluid. It seemed, therefore, that there was selective destruction of pathophysiological activities as well as cessation of synthesis. The activities appeared to be lost at different rates. Mouse-protective activity was lost more rapidly than histaminesensitising and leukocytosis-promoting activities; heat-labile toxicity was lost more slowly or less completely. Modulation was shown to be easily reversed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapidity of antigenic modulation of Bordetella pertussis in modified Hornibrook medium

Idigbe

Parton

Wardlaw

1981

Journal of Medical Microbiology

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“…Previous studies have shown that extracellular concentrations of CAMP are much lower after growth in C media than after growth in X media (Parton & Durham, 1978;Hall et a/., 1982;Brownlie et al, 1984). Low concentrations of extracellular cAMP after growth in C media cannot be accounted for by direct inhibition of adenylate cyclase activity by pro-C-mode salts, as most of these caused no more inhibition than did the pro-X-mode salt NaCI.…”

Section: Discussionmentioning

confidence: 99%

“…, I984), heat-labile toxin, the major cell-envelope polypeptides with M , 28 000 and 30000 (X polypeptides), filamentous haemagglutinin, agglutinogen 1, cytochrome d(,?<, and adenylate cyclase activity. These properties are also lost during a similar change termed phase degradation, which occurs when B. pertussis is repeatedly subcultured in vitro (Leslie & Gardner, 1931 ;Roberts & Ospeck, 1942;Keogh & North, 1948;Eldering et a/., 1962;Aprile, 1972;Parton & Wardlaw, 1975;Parton & Durham, 1978;Dobrogosz et a/., 1979). Until recently this change was thought to be irreversible (Weiss & Falkow, 1984).…”

Section: Introductionmentioning

confidence: 99%

Adenylate Cyclase Activity During Phenotypic Variation of Bordetella pertussis

Brownlie¹,

Coote²,

Parton³

1985

Microbiology

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“…Little is known about the mechanism of the phase variation that occurs in Burdetella species (B. pertussis and B. bronchiseptica) which results in the simultaneous loss of several putative virulence determinants. These include toxins, adenylate cyclase, haemolysins and agglutinins (Ezzell et al, 1981;Wardlaw et af., 1976;Wardlaw & Parton, 1979;Parton & Durham, 1978;Dobrogosz et af., 1979;Nakase, 1957~;Peppler, 1982). The change from virulent phase I to avirulent phase I11 or IV can be rapid (Nakase, 19576;Weiss et al, 1983) and is seldom reversible in vitru (Weiss & Falkow, 1984), though reversion can occur in vivu, at least with the pig pathogen, B. bronchiseptica (Collings & Rutter, 1985).…”

Section: Introductionmentioning

confidence: 95%