Loss and resiliency of social amoeba symbiosis under simulated warming

Shu, Longfei; Qian, Xinye; Brock, Debra A.; Geist, Katherine S; Queller, David C.; Strassmann, Joan E.

doi:10.1002/ece3.6909

Cited by 11 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amoebae are a major group of protists that move through pseudopods and feed by phagocytosis. They are widespread in soil and play a key role in soil nutrient cycling, affect microbial diversity through predation, and have significant ecological and environmental impacts. ,− PS nano- and microplastics were selected in this study because they are one of the common sources of nano- and microplastic in the soil environment and are also commercially available. − Specifically, we conducted amoeba fitness assays, various microscopy observations, and transcriptome sequencing analysis to answer the following two questions: (1) What is the fitness consequence of a soil amoeba D. discoideum in response to nano- and microplastics?…”

Section: Introductionmentioning

confidence: 99%

Complex Bilateral Interactions Determine the Fate of Polystyrene Micro- and Nanoplastics and Soil Protists: Implications from a Soil Amoeba

Zhang

et al. 2022

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Nano-and microplastics have become a serious global concern, threatening our living environments. Previous studies have shown that many organisms, including bacteria, animals, and plants, can be affected by microplastics. However, little is known about one ecologically important group of soil organisms, the protists. In this study, we investigated how polystyrene micro-and nanoplastics interacted with a soil amoeba Dictyostelium discoideum. The results showed that environmental concentrations of nano-and microplastics could negatively affect the soil amoeba's fitness and development. D. discoideum ingested both nano-and microplastics through phagocytosis but packed and excreted them during slug migration, which also promoted their biodegradation. Fourier transform infrared spectroscopy analyses revealed the formation of new oxygen-containing functional groups and the sign of possible oxidation of polystyrene. Also, nano-and microplastic exposure disrupted the nutrient and energy metabolisms of D. discoideum and affected the expression of key genes (e.g., cf45-1, dcsA, aprA, dymB, and gef B) related to morphogenesis and phagocytosis. In conclusion, our results show that nano-and microplastics have complex bilateral interactions with the soil amoeba, affecting each other's fate in the soil environment. This study provides new insights into how soil protists interact with nano-and microplastics in the soil ecosystem.

show abstract

Section: Introductionmentioning

confidence: 99%

Complex Bilateral Interactions Determine the Fate of Polystyrene Micro- and Nanoplastics and Soil Protists: Implications from a Soil Amoeba

Zhang

et al. 2022

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent evidence has also suggested that environmental factors could impact Paraburkholderia prevalence, where P. hayleyella infection provided additional detriment to D. discoideum under artificial warming conditions (Shu et al, 2020). Therefore, our findings regarding symbiont prevalence may not be generalizable to other D. discoideum populations occurring in different soil environments.…”

Section: Discussionmentioning

confidence: 99%

The complexities of inferring symbiont function: Paraburkholderia symbiont dynamics in social amoeba populations and their impacts on the amoeba microbiota

DuBose

Robeson

Hoogshagen

et al. 2021

Preprint

View full text Add to dashboard Cite

The relationship between the social amoeba Dictyostelium discoideum and its endosymbiotic bacteria Paraburkholderia provides a model system for studying the development of symbiotic relationships. Laboratory experiments have shown that any of three species of Paraburkholderia symbiont allow D. discoideum food bacteria to persist through the amoeba lifecycle and survive in amoeba spores, rather than being fully digested. This phenomenon is termed "farming", as it potentially allows spores dispersed to food poor locations to grow their own. The occurrence and impact of farming in natural populations, however, has been a challenge to measure. Here, we surveyed natural D. discoideum populations and found that only one of the three symbiont species, P. agricolaris, remained prevalent. We then explored the effect of Paraburkholdia on the amoeba microbiome, expecting that by facilitating bacterial food carriage it would diversify the microbiome. Contrary to our expectations, Paraburkholderia tended to infectiously dominate the D. discoideum microbiome, in some cases decreasing diversity. Similarly, we found little evidence for Paraburkholderia facilitating the carriage of particular food bacteria. These findings change our understanding of farming and suggest the possibility that Paraburkholderia could be playing multiple roles for its host, as inferred metagenomic analysis indicates a potential role of P. agricolaris in toxin degradation.

show abstract

“…The amoeba proto-farming symbiosis is a promising system to address whether unicellular protist hosts could recognize and discriminate their symbionts ( 19 – 21 , 25 – 28 ). Dictyostelium discoideum is a soil-dwelling amoeba belonging to protozoa and primarily feeding on bacteria, which has been widely used as an ideal system to study cell biology, symbiosis, evolution, and ecology ( 20 , 21 , 23 , 24 , 26 , 28 – 32 ). Amoebas can aggregate and differentiate into pluricellular fruiting bodies upon food-deprived conditions.…”

Section: Introductionmentioning

confidence: 99%

Symbiont-Induced Phagosome Changes Rather than Extracellular Discrimination Contribute to the Formation of Social Amoeba Farming Symbiosis

Tian

Peng

et al. 2022

Microbiol Spectr

Self Cite

View full text Add to dashboard Cite

show abstract

Loss and resiliency of social amoeba symbiosis under simulated warming

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 32 publications

Complex Bilateral Interactions Determine the Fate of Polystyrene Micro- and Nanoplastics and Soil Protists: Implications from a Soil Amoeba

Complex Bilateral Interactions Determine the Fate of Polystyrene Micro- and Nanoplastics and Soil Protists: Implications from a Soil Amoeba

The complexities of inferring symbiont function: Paraburkholderia symbiont dynamics in social amoeba populations and their impacts on the amoeba microbiota

Symbiont-Induced Phagosome Changes Rather than Extracellular Discrimination Contribute to the Formation of Social Amoeba Farming Symbiosis

Contact Info

Product

Resources

About