LOSP: a newly identified sperm protein from<i>Littorina obtusata</i>

Lobov, Arseniy A.; Maltseva, Arina L.; Mikhailova, Natalia; Granovitch, Andrei I.

doi:10.1093/mollus/eyv010

Cited by 13 publications

(21 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this case, it was related with cytosolic vesicles (Figures b and a). This corroborates vesicular localization of LOSP suggested earlier due to the presence of the hydrophobic signal peptide in the LOSP molecule (Lobov et al., ). These cells resemble early stages of so‐called “nurse cells”, or parasperm, of related species Littorina sitkana (Buckland‐Nicks & Chia, ).…”

Section: Resultssupporting

confidence: 91%

“…This study confirmed and supplemented our previous results (Lobov et al., ). LOSP has been proved to be a parasperm protein present in two forms in the sperm‐extracts; its mRNA is expressed only in the testis tissues by parasperm precursor cells.…”

Section: Discussionsupporting

confidence: 94%

“…Based on the extraction details, we initially suggested spermatozoa as a LOSP source, although a possibility of an alternative origin (from parasperm or seminal fluids) was not excluded (Lobov et al., ). Here, we characterize the localization of LOSP in the male reproductive system traced from the presumably paraspermal stem cells in the testis (where losp gene expression was detected using in situ RNA‐hybridization) to protein localization (defined using immunocytochemistry).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

LOSP: A putative marker of parasperm lineage in male reproductive system of the prosobranch mollusk Littorina obtusata

et al. 2018

Self Cite

View full text Add to dashboard Cite

Reproductive isolation is the key attribute of biological species and establishment of the reproductive barriers is an essential event for speciation. Among the mechanisms of reproductive isolation, gamete incompatibility due to the variability of gamete interaction proteins may drive fast divergence even in sympatry. However, the number of available models to study this phenomenon is limited. In case of internally fertilized invertebrates, models to study gamete incompatibility and sperm competition mechanisms are restricted to a single taxon: insects. Here, we propose a group of closely related Littorina species as a new model for such studies. Particularly since periwinkles are already thoroughly studied in terms of morphology, physiology, ecology, phylogeny, and ecological speciation. Earlier, we have identified the first species-specific Littorina sperm protein (LOSP) with no known conservative domains or homologies. LOSP is relatively abundant component of sperm extracts and might be involved in gamete incompatibility. Here, we characterize its definitive localization and mRNA expression pattern in the male reproductive system by immunocytochemistry and RNA in situ hybridization. We demonstrate that LOSP distribution is limited to the parasperm cells. Losp gene expression occurs only at the early stages of parasperm development. The protein is stored within granules of mature parasperm and, most likely, is released after ejaculation inside female reproductive system. Thus, LOSP is the only described molluscan paraspermal protein to date, and there is a possibility for LOSP to be involved in gamete incompatibility since heterospermy is a common phenomenon among Littorina.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

LOSP: A putative marker of parasperm lineage in male reproductive system of the prosobranch mollusk Littorina obtusata

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…During the last decades, Littorina species have become model organisms for the study of population biology, physiological adaptations, genetics, behavior, and parasitology (Johannesson et al, 1997;Sokolova et al 2000, Sokolova andPörtner 2001a,b;Panova and Johannesson, 2004;Panova et al, 2006;Quesada et al, 2007;Mikhailova et al, 2009;Conde-Padín et al 2009Galindo et al, 2010;Storey et al 2013;Lobov et al, 2015;Rolan-Alvarez et al, 2015;Maltseva et al, 2016;Westram et al, 2014;Ravinet et al, 2016, Granovitch, 2016. Along the North Atlantic shore, Littorina saxatilis (Olivi, 1872) and L. obtusata (Linnaeus, 1758) are most common (Reid, 1996).…”

Section: Introductionmentioning

confidence: 99%