Losing Reproduction: Effect of High Temperature on Female Biochemical Composition and Egg Quality in a Freshwater Crustacean with Direct Development, the Red Cherry Shrimp,Neocaridina davidi(Decapoda, Atyidae)

Abstract

Maternal provisioning is particularly important in invertebrates with abbreviated development because large energy reserves must be provided for the developing embryo. In this context, the objective of the present study was to analyze in an aquatic invertebrate with direct development the effect of temperature on female biochemical composition and reserve allocation to maturing ovaries, which determine egg quality. A decapod crustacean, the freshwater shrimp Neocaridina davidi, was used as experimental model. Newly hatched juveniles were exposed to 28 °C or 33 °C. Females showed mature ovaries and spawned at 28 °C (control ovigerous females), but no ovigerous female was found at 33 °C. After a 200-day period, half of the females at 33 °C were transferred to 28 °C, where they rapidly showed mature ovaries and spawned (transferred ovigerous females). Ovigerous females and females that did not spawn at 28 °C (control non-ovigerous females) and at 33 °C (high-temperature non-ovigerous females) were sacrificed to determine their biochemical composition. The number, volume, weight, and biochemical composition of the eggs from transferred and control ovigerous females were also analyzed as indicators of their quality. Female biochemical composition was not influenced by temperature, because control and high-temperature non-ovigerous females had similar lipid, protein, and glycogen contents. However, ovarian maturation and spawning were inhibited at 33 °C, which indicates a negative effect of this temperature on nutrient transfer to the oocytes. This effect was rapidly reversed after females were moved to 28 °C; the eggs from control and transferred ovigerous females were of similar quality, except for a lower protein content in the latter. The present results provide valuable information on reserve allocation to reproduction under thermal stress.

Assessment of potential trade‐off between maternal colouration and offspring quality in the ornamental “red cherry” shrimpNeocaridina davidi(Bouvier)

“…Protein, lipid and glycogen contents in 30‐day‐old offspring were independent of female colouration, and they were amongst the previously reported values for the species (Baliña, Temperoni, López Greco & Tropea, ; Tropea & López Greco, ). In view of these results, maternal colouration does not seem to influence offspring performance.…”

“…Protein, lipid and glycogen contents in 30-day-old offspring were independent of female colouration, and they were amongst the previously reported values for the species (Baliña, Temperoni, López Greco & Tropea, 2018;. differences were found with a diet supplemented with 400 mg/ kg (Yamada, Tanaka, Sameshima & Ito, 1990).…”

Effect of commercial diets on female reproductive performance and offspring quality in the red cherry shrimpNeocaridina davidi(Caridea, Atyidae)

“…Furthermore, egg hatching rate and larval tolerance to ammonia were significantly improved by the inclusion of n‐3 PUFAs in the diet of M. rosenbergii broodstock, whereas fecundity increased with increasing levels of dietary LIN (C18:2 n‐6; Cavalli et al., 1999). In this species, as well as in N. davidi , n‐6 PUFAs levels in tissues were more abundant than n‐3 PUFAs (C20:5 n‐3‐ EPA‐ and C22:6 n‐3‐ DHA‐; Baliña et al., 2018). It was proposed that N. davidi females derive LIN to oocytes during vitellogenesis for its use by embryos as a DHA precursor.…”

“…The first diet was balanced food for tropical fish, which was the only one previously tested for N. davidi reproduction and growth (e.g. Baliña, Temperoni, López Greco, & Tropea, 2018; Marciano et al., 2018; Tropea & López Greco, 2015). The second one was a commercial diet specifically formulated for crustaceans with a similar market value, and the third one was a commercial diet for ornamental fish which had a lower cost than the others.…”

Effect of different frozen fresh diets to broodstock growth, reproductive performance and larvae of cleaner shrimp, Lysmata amboinensis

“…The results obtained in the present study suggested that high concentration of monounsaturated fatty acid (MUFA) with focusing in significant higher content of 16:1 (Palmitoleic) in mussel, may assist metabolic functioning and support energy requirements during the embryonic development which finally could be associated for better egg retention and high egg production compared to other diets. As mentioned by Balina et al (2018), reproductive event in broodstocks uses great amount of energy. It is broadly acknowledged that MUFA is utilized to provide energetic function (Cavalli et al, 2001;Rosa et al, 2003).…”