Losartan prevents bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder

Abstract

Aims: To investigate the effect of losartan on preventing bladder fibrosis and protecting renal function in rats with neurogenic paralysis bladder (NPB). Materials and Methods: Rats were assigned to the transecting spinal nerves group (TSNG), transecting spinal nerves + losartan group (LSTG), and control group (CG). On Day 32 postsurgery, bladder capacity (BC), bladder compliance (ΔC), bladder leakage pressure (P ves.leak) of TSNG and LSTG while BC, ΔC, and bladder threshold pressure (P ves.thre) of CG were measured by cystometry in each cohort. Renal function and the expression quantity of Angiotensin Ⅱ (Ang II) in blood were detected, in addition Ang II, Ang II Type 1 receptor (AT1), transformation growth factor β1 (TGFβ1), Collagen Ⅲ, and collagen fibrin in the bladder tissue were detected too. Results: ΔC in TSNG and LSTG decreased significantly compared to the CG. P ves.leak in TSNG and LSTG were significantly higher than P ves.thre in CG. Renal function of both TSNG and LSTG decreased significantly compared with the CG, but renal function in LSTG was better than in TSNG. Ang Ⅱ in blood and bladder tissue in TSNG and LSTG increased significantly compared with CG. AT1 was expressed in the bladder tissue of all rats. The TGFβ1, Collagen Ⅲ, and collagen fibrin expression level increased significantly in TSNG compared with LSTG and CG, while these levels were not significantly different between CG and LSTG. Conclusion: Losartan might prevent NPB fibrosis by stopping the upregulated signaling of Ang II/AT1/TGFβ1 and consequently may reduce kidney damage from occurring.

Tengdan Capsule Prevents Hypertensive Kidney Damage in SHR by Inhibiting Periostin-Mediated Renal Fibrosis

“…This is consistent with the previous findings by others. For example, losartan alleviates renal fibrosis in rats with nephrectomy by down-regulating HIF-1alpha and up-regulating MMP-1/TIMP-1 or through inhibition of ER stress via up-regulation of SIRP1, followed by induction of HO-1 and thioredoxin ( He et al, 2014 ; Kim et al, 2017 ); it attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy ( Song et al, 2019 ) and accelerates the repair process of renal fibrosis in UUO mouse after the surgical recanalization by upregulating the Tregs expression ( He et al, 2021 ). Most recently, losartan is shown to prevent bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder ( Norris et al, 2007 ).…”

ABT-263 exerts a protective effect on upper urinary tract damage by alleviating neurogenic bladder fibrosis

“…Although He et al. [ 24 ] found a protective effect with four weeks of ‘losartan’ treatment on bladder fibrosis and renal function, a long-term study of treatment observation after NBF is lacking.…”

“…The anterior and posterior diameters of the renal pelvis were measured using a multifunctional Doppler ultrasound (LOGIQ E9; GE, Boston, MA, USA) and a 4–15 MHz probe to evaluate hydronephrosis. Rats were anaesthetized by intraperitoneal injection of urethane [ 23 ] at a concentration of 20% and a dose of 1 g/kg, and a blunt epidural catheter with a diameter of 1 mm was smeared with a paraffin oil globule and inserted into the bladder for cytometry, as described in our previous publication [ 24 ]. Bladder pressure curves and basal bladder pressure, P ves.thre (bladder pressure just before voiding), P ves.leak (pressure in the bladder when filling incontinence occurs), P ves.max (maximum pressure during voiding), voiding interval, and time to first leakage were recorded.…”

Telmisartan inhibits bladder smooth muscle fibrosis in neurogenic bladder rats

“…It is generally believed that bladder wall fibrosis plays an important role in NB ( 26 ). In the present study, the Masson and H&E staining results showed thickening of the bladder wall, disordered and hypertrophic detrusor cells, and proliferative collagen fibers in NB model rats.…”