Lord and Lady Nelson: Some Unpublished Letters

Heat shock proteins: molecular chaperones of protein biogenesis.

“…It is likely that each Ssap is optimized for a specific activity or activities in the cell but that the other members have the ability to substitute for each other. This idea is consistent with the observation that Ssa3p and Ssa4p can maintain the viability of a ssal ssa2 strain but are unable to completely suppress the temperature sensitive phenotype even when overepressed (103).…”

“…Thus the activities of the molecular chaperones and the proteolytic machinery are likely to be intimately entwined. This notion is supported by the observation that yeast ssal ssa2 mutants are sensitive to the amino acid analog canavanine, which, upon incorporation into proteins, presumably causes misfolding (103).…”