Loop-mediated isothermal amplification of the toxR gene coupled with lateral flow dipstick (LAMP-LFD) for the novel, rapid and specific visual detection of Vibrio harveyi
Rahman, A. M. A.,
Ransangan, J.,
Subbiah, V. K.
Abstract:Aims: Vibrio harveyi is a serious pathogen for marine organisms particularly in hatcheries and grow-out ponds that attack their immune system. The rapid detection of V. harveyi is urgently needed to prevent bacterial spread. Here we described a rapid and specific visual detection method based on the Loop-Mediated Isothermal Amplification in combination with Lateral Flow Dipstick (LAMP-LFD). Methodology and results: A set of six novel primers were designed to target the toxR gene. These include the biotinlabell… Show more
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