Loop-mediated isothermal amplification (LAMP) and machine learning application for early pregnancy detection using bovine vaginal mucosal membrane

Kunii, Hiroki; Kubo, Tomoaki; Asaoka, Natsuki; Balboula, Ahmed Z.; Hamaguchi, Yu; Shimasaki, Tomoya; Bai, Hanako; Kawahara, Manabu; Kobayashi, Hisato; Ogawa, Hidehiko; Takahashi, Masashi

doi:10.1016/j.bbrc.2021.07.015

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…For the last decade and particularly recently, substantial data on pregnancy detection in cattle have become available: ISGs expression in PBMCs and PMNs [8], CL examination by Doppler ultrasonography [10], and microRNA in extracellular vesicles [12]. In addition, several studies have recently been attempting to identify specific markers for discriminating success or failure of pregnancy in ruminants [22][23][24][25][26][27][28]. However, these methods are not yet applicable in the field.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers

Kusama

Bai

Matsuno

et al. 2022

Life

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Pregnancy loss predominantly occurs during the first 3–4 weeks due to fertilization failure or early embryonic losses in cattle. Insufficient biochemical communication between conceptus (embryo plus extraembryonic membranes) and endometrium has been suspected as the primary cause for early embryonic losses. If molecules regulating this communication were identified, molecular mechanisms associated with early pregnancy losses could be better understood. To identify candidate molecules as detection markers of non-pregnant or females undergoing embryonic loss, peripheral blood from embryo-transferred heifers on day 7 (day 0 = day of estrus) were collected on days 17 (pre-attachment), 20 (during attachment), and 22 (post-attachment), which were subjected to metabolome and global proteome iTRAQ analyses. The metabolome analysis partly divided serum components into pregnant or not. In the iTRAQ analysis, heatmap analysis with top 25 proteins was separated into pregnant or not on day 20 or 22. Furthermore, receiver operating characteristic curve (ROC) analysis identified five candidate proteins detecting non-pregnant heifers, of which SNX5 in day 22 serum had the highest area under the curve (AUC): 0.983. We also detected SNX5 in day 22 serum from non-pregnant heifers using western blotting. These results suggest that high SNX5 in day 22 serum could predict early pregnancy loss in heifers.

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Section: Discussionmentioning

confidence: 99%

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers

Kusama

Bai

Matsuno

et al. 2022

Life

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“…Another MUC family protein, MUC16, has a similar expression profile; MUC16 expression is abrogated during the receptive phase in the human endometrium [72], and its expression is lower in women who conceived than that in women who did not [73]. MUC16 expression is higher in repeat breeder cows and can be used as a marker of difficulty in becoming pregnant [74]. Taken together, these studies suggest that removal of MUC proteins is a prerequisite for uterine receptivity and subsequent conceptus implantation in mammals.…”

Section: Projection Of These Molecular Mechanisms To Other Mammalsmentioning

confidence: 99%

Molecular approaches to mammalian uterine receptivity for conceptus implantation

KUBOTA

2024

J. Reprod. Dev.

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Mammalian reproduction is more inefficient than expected and embryo/conceptus implantation into the maternal endometrium is considered to be a rate-limiting process. Although extensive physiological and structural diversity exists among mammalian species, the basic molecular mechanisms underlying successful implantation are conserved. The extensive use of genetically engineered mouse models has provided considerable information on uterine receptivity for embryo implantation. The molecular mechanisms and cellular processes identified thus far require further validation in other mammalian species. In this review, representative ovarian steroid hormone-induced signaling pathways controlling uterine adaptation are presented based on the results of rodent studies.Selected examples of functional conservation in mammals, such as humans and cattle, are briefly described. To date, molecular therapeutic trials for fertility improvement have not been conducted.Considerable efforts are required to provide further understanding of these molecular mechanisms. Such understanding will contribute to the development of reliable clinical diagnostics and therapeutics for implantation failure, leading to reproductive success in a wide variety of mammals in the future.

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“…Recently, we identified ISGs expression in bovine tissues, such as the cervical and vaginal mucosa, from which a less invasive pregnancy diagnosis was developed. [ 114 , 115 ]. It appears that the expression of ISGs is seen in humans, mice, and cows, and future validation is needed to understand the species-specific types as well as those common across mammalian species.…”

Section: Common Maternal Responses Exist During the Early Mid-pregnan...mentioning

confidence: 99%

Recent progress of interferon-tau research and potential direction beyond pregnancy recognition

et al. 2022

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Since the discovery of interferon-tau (IFNT) over 30 years ago as the trophectodermal cytokine responsible for the maintenance of the maternal corpus luteum (CL) in ruminants, exhaustive studies have been conducted to identify genes and gene products related to CL maintenance.Recent studies have provided evidence that although CL maintenance, with the up-and downregulation of IFNT, is important, its regulatory role in the endometrial expression of interferonstimulated genes (ISGs) is far more important for conditioning the uterine environment for successful conceptus implantation and thereafter. This review initially describes the mammalian implantation process, briefly but focuses on recent findings, as there appears to be a common phenomenon during early to mid-pregnancy among mammalian species.

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Loop-mediated isothermal amplification (LAMP) and machine learning application for early pregnancy detection using bovine vaginal mucosal membrane

Cited by 6 publications

References 20 publications

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers

Molecular approaches to mammalian uterine receptivity for conceptus implantation

Recent progress of interferon-tau research and potential direction beyond pregnancy recognition

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