Loop-Mediated Isothermal Amplification (LAMP), An Innovation in Gene Amplification: Bridging the Gap in Molecular Diagnostics; A Review

Abdullahi, Umar Faruk; Naim, Rochman; Taib, Wan Rohani Wan; Saleh, Aminu; Muazu, Anas; Aliyu, Sani; Baig, Atif Amin

doi:10.17485/ijst/2015/v8i17/55767

Cited by 36 publications

(33 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These stem loop structure pay way to auto cyclic amplification by involving loop primers which shorten time and increase sensitivity. Bst and Bsm DNA polymerase is the enzyme play key role in LAMP amplification process [1]. The LAMP reaction process proceeds at isothermal conditions vary from 60 to 65°C for 30-60 min [9,12,18].…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay

2017

View full text Add to dashboard Cite

A loop-mediated isothermal amplification (LAMP) technique was employed to develop a simple and rapid method for the detection of tomato leaf curl Bangalore virus (ToLCBaV) in diseased plants of tomato (). Six sets of primers were designed for LAMP technique targeting the conserved AC1 region and successfully detected ToLCBaV. No reaction was detected in the tissues of healthy plants by either the LAMP or the polymerase chain reaction (PCR). The LAMP products can be visualized by presence or absence of turbidity and staining (0.2 μL for 25 μL LAMP product) directly in the tube with nucleic acid stain dye which allowed easy detection. Sensitivity of LAMP assay is 100 times of conventional PCR technique. Although, both the LAMP and the PCR methods were capable of detecting ToLCBaV in infected tissues of tomato, the LAMP method would be more useful than the PCR method for detection of ToLCBaV infection in tomato plants because it is more rapid, simple and accurate method.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay

2017

View full text Add to dashboard Cite

show abstract

“…The most vital step in the LAMP protocol is primer design. Usually, four to six primers are employed to specifically identify six to eight different regions of the target gene, and thereby amplify the gene with highly efficient precision [102]. The strand displacement activity is unique to the LAMP polymerase enzyme, which does not have an exonuclease activity at the 5 -3 , and therefore leads to the production of ssDNA [103].…”

Section: Other Isothermal Amplification Methodsmentioning

confidence: 99%

Current and Emerging Methods for the Synthesis of Single-Stranded DNA

Hao

Qiao

2020

Genes

View full text Add to dashboard Cite

Methods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA. Some approaches allow the synthesis of freely chosen user-defined ssDNA sequences to overcome the restrictions and limitations of different length, purity, and yield. In this perspective, we provide an overview of the representative ssDNA production strategies and their most significant challenges to enable the readers to make informed choices of synthesis methods and enhance the availability of increasingly inexpensive synthetic ssDNA. We also aim to stimulate a broader interest in the continued development of efficient ssDNA synthesis techniques and improve their applications in future research.

show abstract

“…In this LAMP version, all of the reagents are combined into a single mixture, called the lyophilized LAMP mix, in some cases associated with a closed amplification and detection system, in order to simplify and make the LAMP process more rapid; the user only needs to add water and sample or DNA/RNA template into the lyophilized mix before to carry out the incubation at the desired temperature. Many lyophilized LAMP kits are commercially available for rapid diagnosis of many diseases, making it usable under field conditions, facilitating the provision of the an-hour testing strategy in even the most remote rural health facilities, and achieving great diagnostic levels in resource-poor areas [182,183]. These kits are usually associated with portable thermal cyclers, which allow wireless connection with mobile devices (such as smartphones or tablets) for viewing data in real time.…”

Section: Lyophilized Lampmentioning

confidence: 99%

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

Panno

Matić

Tiberini³

et al. 2020

Plants

141

111

View full text Add to dashboard Cite

In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.

show abstract

Loop-Mediated Isothermal Amplification (LAMP), An Innovation in Gene Amplification: Bridging the Gap in Molecular Diagnostics; A Review

Cited by 36 publications

References 90 publications

Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay

Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay

Current and Emerging Methods for the Synthesis of Single-Stranded DNA

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

Contact Info

Product

Resources

About