Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

Li, Weilan; Lee, Seung‐Yeol; Back, Chang–Gi; Ten, Leonid N.; Jung, Hee‐Young

doi:10.5423/ppj.oa.07.2019.0197

Cited by 19 publications

(11 citation statements)

References 20 publications

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“…The first study on the detection of plant diseases was published by Fukuta et al nearly two decades ago, in 2003 [28]. The study concerned the Japanese yam mosaic virus (JYMV) and was conducted using RT-LAMP, which allows for quick, simple, and very sensitive identification of the RNA of plant viruses, as well as viroids, fungi, bacteria, and oomycetes [15,26,[29][30][31][32][33][34][35]. The speed and specificity of LAMP-based tests mean that they can be performed even on-site to roll back a contaminated product immediately and prevent the pathogen from spreading.…”

Section: Detection Of Plant Pathogensmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Soroкa

Wasowicz

Rymaszewska

2021

Cells

194

100

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In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.

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Section: Detection Of Plant Pathogensmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Soroкa

Wasowicz

Rymaszewska

2021

Cells

194

100

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“…This fast, inexpensive method has been recently modified to detect plant viruses, fungi, and bacteria. [87][88][89] An appropriate diagnosis requires high specificity and sensitivity methods, particularly for the early identification of a pathogen. Like PCR, LAMP can detect a single target in a complex matrix, or multiple targets from it.…”

Section: Visual Lampmentioning

confidence: 99%

Next‐generation methods for early disease detection in crops

Trippa,

Scalenghe,

Basso

et al. 2023

Pest Management Science

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BackgroundPlant pathogens are commonly identified in the field by the typical disease symptoms that they can cause. The efficient early detection and identification of pathogens are essential procedures to adopt effective management practices that reduce or prevent their spread in order to mitigate the negative impacts of the disease. Herein, in this review were presented and discussed the traditional and innovative methods for early detection of the plant pathogens highlighting their major advantages and limitations.Resultstraditional techniques of diagnosis used for plant pathogen identification are focused typically on the DNA, RNA (when molecular methods), and proteins or peptides (when serological methods) of the pathogens. Serological methods based on mainly enzyme‐linked immunosorbent assay (ELISA) are the most common method used for pathogen detection due to their high‐throughput potential and low cost. This technique is not particularly reliable and sufficiently sensitive for many pathogens detection during the asymptomatic stage of infection. For non‐cultivable pathogens in the laboratory, nucleic acid‐based technology is the best choice for consistent pathogen detection or identification. Lateral flow systems are innovative tools that allow fast and accurate results even in the field conditions, but they have sensitivity issues to be overcome. PCR assays performed on last‐generation portable thermocyclers may provide rapid detection results in situ.ConclusionThe advent of portable instruments can speed pathogen detection, reduce commercial costs, and potentially can revolutionize plant pathology. This review provides information on current methodologies and procedures for the effective detection of different plant pathogens.This article is protected by copyright. All rights reserved.

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“…This technique has been proven to be more sensitive than traditional techniques of culture and microscopy and PCR that are presently used [ 47 , 48 , 49 ]. It is rapid, does not require expensive equipment, and is ideal for future diagnoses, especially in developing countries [ 50 , 51 ]. To date, only one LAMP assay has been developed to detect T. tenax in human and/or canine oral samples, which can be directly used for clinical samples without prior DNA extraction.…”

Section: Resultsmentioning

confidence: 99%

Trichomonas tenax: A Neglected Protozoan Infection in the Oral Cavities of Humans and Dogs—A Scoping Review

et al. 2023

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Trichomonas tenax is a flagellated protozoan parasite found in the oral cavities of humans and animals and has been associated with periodontal disease, the most prevalent inflammatory disease affecting them all. Studies have shown that T. tenax can cause damage to mammalian cells and secretes virulent proteins, such as cysteine. It is presently considered zoonotic. Despite the few studies that have been done, the pathogenicity of this oral protozoan is still not fully understood. A database search was performed in July 2022 using PubMed and Google Scholar to retrieve data eligible for this study. PRISMA-ScR guidelines were followed to conduct this scoping review. A total of 321 articles were found with 87 included in this review after applying the exclusion criteria. Due to its increasing prevalence worldwide in both humans and dogs, detecting and elucidating the pathogenicity of this parasite is paramount for effective global control and prevention of periodontal disease. However, there is a paucity in the literature on this neglected zoonotic trichomonad, which is in large contrast to the closely related human pathogen T. vaginalis. Here, we comprehensively review the history, morphology and reproduction, host, prevalence, diagnosis, pathogenicity, control, and prevention of T. tenax. Hopefully, this article will call attention to both medical and veterinary professionals as well as epidemiologists on this most neglected and zoonotic protozoan. More epidemiological and clinical studies need to be conducted on T. tenax to gain a better understanding of its pathogenicity, to increase the chances of developing effective drugs to aid in the control of this oral parasite, and reduce the spread of periodontal disease worldwide.

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Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

Cited by 19 publications

References 20 publications

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Next‐generation methods for early disease detection in crops

Trichomonas tenax: A Neglected Protozoan Infection in the Oral Cavities of Humans and Dogs—A Scoping Review

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