Loop-mediated isothermal amplification for the rapid detection of Chrysanthemum chlorotic mottle viroid (CChMVd)

Park, Jungan; Jung, Yuchul; Kil, Eui-Joon; Kim, Jaedeok; Tran, Dung Thi; Choi, Seung‐Kook; Yoon, Ju-Yeon; Cho, Won Kyong; Lee, Sukchan

doi:10.1016/j.jviromet.2013.05.016

Cited by 19 publications

(6 citation statements)

References 24 publications

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“…Reverse transcription LAMP (RT‐LAMP) has been used for the detection of several viroids including peach latent mosaic viroid (PLMVd) (Boubourakas et al ., ), PSTVd (Tsutsumi et al ., ; Lenarcic et al ., ), chrysanthemum chlorotic mottle viroid (CChMVd) (Park et al ., ) and CCCVd (Thanarajoo et al ., ). Liu et al .…”

Section: Methods For Viroid Detectionmentioning

confidence: 99%

“…Reverse transcription LAMP (RT-LAMP) has been used for the detection of several viroids including peach latent mosaic viroid (PLMVd) (Boubourakas et al, 2009), PSTVd (Tsutsumi et al, 2010;Lenarcic et al, 2013), chrysanthemum chlorotic mottle viroid (CChMVd) (Park et al, 2013) and CCCVd (Thanarajoo et al, 2014). Liu et al (2014) developed multiplex RT-LAMP for the simultaneous detection of two major chrysanthemum pathogens, CSVd and chrysanthemum virus B (CVB).…”

Section: Rt-qpcrmentioning

confidence: 99%

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Diagnostic techniques for viroids

et al. 2016

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The fast growth of the human population forces us to produce more food, but higher crop production also leads to the fast spread of diseases. Plant pathology deploys a wide range of methods that do not provide an adequate solution to all disease losses. In the case of viroids, therapeutic means of control are not available; therefore control strategies are more focused on the development of reliable detection methods to quickly exclude the infected plant material. Although viroids are the smallest and simplest plant pathogens, their identification and detection is not straightforward. Each viroid–host combination is specific, and for reliable identification, all steps from sampling to final detection must be performed accurately. In this review, several methods for viroid detection in various host plants are discussed, including their advantages and disadvantages. Even though relatively new molecular methods enable fast and sensitive detection of viroids, a combination of different methods gives the most reliable identification. Techniques based on nucleic acids may be the future for viroid detection but they still cannot replace biological indexing, which is usually essential in epidemiological and aetiological studies.

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Section: Methods For Viroid Detectionmentioning

confidence: 99%

Section: Rt-qpcrmentioning

confidence: 99%

Diagnostic techniques for viroids

et al. 2016

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“…3b). The assay was repeated using the same dilution series in a RotorGene Q The copy numbers of the trRNA dilution series amplification curves are coloured as follows; 6.1 × 10 12 -red, 6.1 × 10 11 -green, 6.1 × 10 10 yellow, 6.1 × 10 9 -blue, 6.1 × 10 8 -pink, 6.1 × 10 7 -black, 6.1 × 10 6orange, 6.1 × 10 5 -purple RT-LAMP assays have been designed previously to detect sequence variants using degenerate bases and mismatches to particular variants in primers with success (James et al 2010;Park et al 2013;Komatsu et al 2015;Tangkanchanapas et al 2018). A similar approach was employed in this study to cover as many CEVd variants as possible, and the results support other studies where RT-LAMP was shown to be a successful diagnostic tool for viroid detection in plants (Tangkanchanapas et al 2018;Verma et al 2020;Tseng et al 2021).…”

Section: Detection Comparison Between Rt-lamp With Rt-qpcr Assaysmentioning

confidence: 99%

A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus

Chambers

Geering

Holford

et al. 2023

Australasian Plant Pathol.

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Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid.

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“…LAMP technology uses the chain replacement activity of Bst DNA polymerase to provide the reaction power and completes the efficient, specific and sensitive amplification of target DNA in a short time under constant temperature conditions. Park et al used LAMP technology to rapidly detect CChMVd at 65 °C [ 109 ]. Liu et al designed four LAMP-specific primers that could identify six specific regions of the TAV CP sequence, which could quickly and accurately detect TAV in chrysanthemums and had advantages of higher sensitivity, speed and convenience than RT-PCR [ 110 ].…”

Section: Research Progress In Chrysanthemum Virus Detection Technologymentioning

confidence: 99%

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Gao,

Chen,

Pan

et al. 2023

Viruses

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Chrysanthemum is an important functional plant that is used for food, medicine and tea. Functional chrysanthemums become infected with viruses all around the world, seriously lowering their quality and yield. Viral infection has become an important limiting factor in chrysanthemum production. Functional chrysanthemum is often propagated asexually by cutting during production, and viral infection of seedlings is becoming increasingly serious. Chrysanthemums can be infected by a variety of viruses causing different symptoms. With the development of biotechnology, virus detection and virus-free technologies for chrysanthemum seedlings are becoming increasingly effective. In this study, the common virus species, virus detection methods and virus-free technology of chrysanthemum infection are reviewed to provide a theoretical basis for virus prevention, treatment and elimination in functional chrysanthemum.

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Loop-mediated isothermal amplification for the rapid detection of Chrysanthemum chlorotic mottle viroid (CChMVd)

Cited by 19 publications

References 24 publications

Diagnostic techniques for viroids

Diagnostic techniques for viroids

A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

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