Loop‐mediated isothermal amplification for rapid identification of biotypes B and Q of the globally invasive pest <i>Bemisia tabaci</i>, and studying population dynamics

Hsieh, Chi-Hsun; Wang, Hurng‐Yi; Chen, Young-Fa; Ko, Chiun‐Cheng

doi:10.1002/ps.3298

Cited by 35 publications

(31 citation statements)

References 28 publications

(37 reference statements)

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“…Kontsedalov et al 15. and Hsieh et al 16. both found that the B biotype dominated crops in open fields and the Q biotype dominated crops in protected agricultural areas.…”

Section: Discussionmentioning

confidence: 99%

“…However, Kontsedalov et al 15. speculated that the high fitness rate for the B biotype and the inefficiency of insecticide control contributed to the predominance of the B biotype in open fields, whereas Hsieh et al 16. regarded temperature as the main impact factor.…”

Section: Discussionmentioning

confidence: 99%

“…These differences contribute to the competitive outcomes between the two biotypes in various habitats. The B biotype is more adapted to open fields, whereas the Q biotype is more competitive in protected agricultural facilities1516.…”

mentioning

confidence: 99%

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Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005–2014

Yao

Zheng

Huang³

et al. 2017

Sci Rep

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The whitefly Bemisia tabaci (Gennadius) is an important agricultural insect pest worldwide. The B and Q biotypes are the two most predominant and devastating biotypes prevalent across China. However, there are few studies regarding the occurrence of the Q biotype in Fujian Province, China, where high insecticide resistance has been reported in the B biotype. Differences in some biological characteristics between the B and Q biotypes, especially insecticide resistance, are considered to affect the outcome of their competition. Extensive surveys in Fujian revealed that the B biotype was predominant during 2005–2014, whereas the Q biotype was first detected in some locations in 2013 and widely detected throughout the province in 2014. Resistance to neonicotinoids (that have been used for more than 10 years) exhibited fluctuations in open fields, but showed a continual increasing trend in protected areas. Resistance to lambda-cyhalothrin, chlorpyrifos, and abamectin exhibited a declining trend. Resistance to novel insecticides, such as nitenpyram, pymetrozine, sulfoxaflor, and cyantraniliprole, in 2014 was generally below a moderate level. A decline in insecticide resistance in the B biotype and the rapid buildup of protected crops under global temperature increase may have promoted the establishment of the Q biotype in Fujian.

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“…Kontsedalov et al 15. and Hsieh et al 16. both found that the B biotype dominated crops in open fields and the Q biotype dominated crops in protected agricultural areas.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005–2014

Yao

Zheng

Huang³

et al. 2017

Sci Rep

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“…A research priority is to employ new technologies to deliver rapid diagnostic tools that can be used in less developed countries where laboratory infrastructure and human capacity is often limited. The aforementioned use of LAMP-based diagnostic systems offers great scope for addressing the challenge of sweetpotato pests, for example discrimination of whitefly biotypes (Hsieh et al, 2012), as well as for plant disease issues. This capacity would be useful in detection of viruses and phytoplasmas in PT schemes and differentiating between pest species with morphologically similar life stages.…”

Section: Conclusion and Research Recommendationsmentioning

confidence: 99%

Invertebrate pests and diseases of sweetpotato (Ipomoea batatas): a review and identification of research priorities for smallholder production

Johnson

Gurr

2016

Ann Appl Biol

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Sweetpotato has been the subject of little research worldwide compared with other major crop staples, and this is especially so for less developed countries where sweetpotato is critical for food security. This review synthesises information on plant protection issues that affect smallholder sweetpotato farmers in less developed countries to identify major issues and suggest research priorities. Though the pests and diseases of sweetpotato in less developed countries are largely common to industrialised systems, their relative importance differs and losses tend to be more severe as a result of differing agronomic practices and relative unavailability of management options and technical support that are important in developed countries. Smallholders are heavily reliant on cultural practices such as traditional forms of biological control using ants and livestock, fallowing and composting (sometimes with plant materials having biocidal properties). Crop protection methods that have been developed for use in sweetpotato production in developed countries, such as pathogen-tested planting material, early maturing varieties, pheromone trapping and pesticides are less accessible to, and relevant for, smallholders. Smallholders also typically harvest a given crop progressively which extends the period over which storage roots are potentially vulnerable to attack but reduces the risk of post-harvest losses. Human population growth in developing countries is leading to an increase in cropping intensity with shorter fallow periods and more years of continuous crops. This has the dual effect of depleting soil nutrients and increasing the potential for pest and pathogen build-up. Associated with this, the adoption of strategies to manage crop nutrition, such as not burning crop residues, promote carryover of pests and pathogen inocula. As a consequence of these factors, sweetpotato yield losses from diseases, especially viruses, and pests, particularly weevils, can be high. Climate change is likely to result in more frequent drought and this will increase losses caused by sweetpotato weevils that are favoured by dry conditions. This review of sweetpotato pests and their management options concludes with suggestions for some future research priorities including the combination of traditional practices that have pest management outcomes with relevant practices from industrial production that are able to be transferred or modified for use in smallholder production. Increased technical support for decision making and diagnostics, including molecular approaches that have scope for field use, will be important in reducing the burden imposed by biotic threats to this important global crop.

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“…To identify the biotypes of the whiteflies trapped by the Y-plates, we collected single whiteflies from the plates and mixed their homogenates with the reaction mixture from a commercial biotype detection kit (Nippon Gene, Tokyo, Japan) for a loop-mediated isothermal amplification (LAMP) of specific genome sequences of the whiteflies (Hsieh et al, 2012). To detect TYLCV in the trapped whiteflies, we pierced the whiteflies with sterilized toothpicks and then dipped the toothpicks in solution from a commercial TYLCV-detection kit (Nippon Gene) for LAMP of viral DNA (Fukuta et al, 2003).…”

Section: Biotype Determination Of Trapped Whiteflies and Virus Detectmentioning

confidence: 99%

Prevention of Whitefly Entry from a Greenhouse Entrance by Furnishing an Airflow-Oriented Pre-Entrance Room Guarded with Electric Field Screens

Nonomura¹,

Matsuda²,

Kakutani³

et al. 2014

JAS

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Two types of an electric field screen were used to exclude whiteflies from a greenhouse. Singly charged dipolar electric field screen had insulated conductor iron wires arrayed in parallel (ICW-layer), two earthed metal nets on both sides of the ICW-layer, and a direct current voltage generator. Screens were attached to the lateral windows of the greenhouse to repel whiteflies that approached the nets. To electrostatically guard the greenhouse entrance, doubly charged dipolar electric field screens (DD-screens) were used to capture whiteflies entering through the door. The ICWs, oppositely charged with equal voltages, were arrayed one after the other, and an insulator board or net was placed on one side of the ICW-layer. ICWs captured whiteflies entering an electric field of DD-screens. A small pre-entrance room was constructed at the entrance area, and three DD-screens (with yellow and gray board or gray net) were installed in the pre-entrance room taking into consideration the airflow inside the room, as most whiteflies were brought in by the air when the door was opened. Two DD-screens with the board were useful for directing the wind toward the wall into which the netted DD-screen was integrated. Insects were eliminated by this screen, and the pest-free air was circulated inside the greenhouse. The yellow-boarded DD-screen was highly attractive because of the photo-selective movement of the whiteflies and was effective for trapping the whiteflies when there was no wind. Our electrostatic method is effective at keeping the greenhouse pest-free throughout the entire period of tomato cultivation.

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Loop‐mediated isothermal amplification for rapid identification of biotypes B and Q of the globally invasive pest Bemisia tabaci, and studying population dynamics

Abstract: The newly designed LAMP assay is a simple, rapid and accurate method for identifying the B and Q biotypes. It can be conducted by non-specialists and can contribute to pest management.

Cited by 35 publications

References 28 publications

Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005–2014

Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005–2014

Invertebrate pests and diseases of sweetpotato (Ipomoea batatas): a review and identification of research priorities for smallholder production

Prevention of Whitefly Entry from a Greenhouse Entrance by Furnishing an Airflow-Oriented Pre-Entrance Room Guarded with Electric Field Screens

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