Loop-Mediated Isothermal Amplification Assay for Peste des Petits Ruminants Virus Detection and its Comparison with RT-PCR

Molecular evidence and hematological alterations associated with the occurrence of coronavirus in domestic dogs in Pakistan

“…Theses discrepancies might be due to the instability of RNA virus in the fecal material due to activities of endogenous RNases. Lack of accessibility of partially degraded RNA or intermittent shedding of the virus in feces may influence the sensitivity of PCR assay (Vermeulen et al, 2011;Niamat et al, 2019). Furthermore, 53.3 % of the PCR positive dogs were of Pugs breeds, this breed was considered the most susceptible to CCoV, with the disease being more severe and having a worse prognosis if animals will be having other respiratory problems (Brandell & Olson, 2020).…”