Loop formation and self-fasciculation of cortical axon using photonic guidance at long working distance

Mondal, Angana; Black, Bryan; Kim, Young Tae; Mohanty, Samarendra

doi:10.1038/srep06902

Cited by 9 publications

(3 citation statements)

References 48 publications

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“…Recently, we found purely-optical repulsive-guidance of primary axons by near infrared (NIR) light to be highly effective 13 14 . The ability to guide axons using purely-optical attractive 3 6 11 or repulsive 13 14 means is very promising due to light’s potential for high spatial 15 and temporal selectivity, its absolute sterility, and for its minimal invasiveness.…”

mentioning

confidence: 99%

Spatial temperature gradients guide axonal outgrowth

Black¹,

Vishwakarma²,

Dhakal³

et al. 2016

Sci Rep

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Formation of neural networks during development and regeneration after injury depends on accuracy of axonal pathfinding, which is primarily believed to be influenced by chemical cues. Recently, there is growing evidence that physical cues can play crucial role in axonal guidance. However, detailed mechanism involved in such guidance cues is lacking. By using weakly-focused near-infrared continuous wave (CW) laser microbeam in the path of an advancing axon, we discovered that the beam acts as a repulsive guidance cue. Here, we report that this highly-effective at-a-distance guidance is the result of a temperature field produced by the near-infrared laser light absorption. Since light absorption by extracellular medium increases when the laser wavelength was red shifted, the threshold laser power for reliable guidance was significantly lower in the near-infrared as compared to the visible spectrum. The spatial temperature gradient caused by the near-infrared laser beam at-a-distance was found to activate temperature-sensitive membrane receptors, resulting in an influx of calcium. The repulsive guidance effect was significantly reduced when extracellular calcium was depleted or in the presence of TRPV1-antagonist. Further, direct heating using micro-heater confirmed that the axonal guidance is caused by shallow temperature-gradient, eliminating the role of any non-photothermal effects.

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mentioning

confidence: 99%

Spatial temperature gradients guide axonal outgrowth

Black¹,

Vishwakarma²,

Dhakal³

et al. 2016

Sci Rep

Self Cite

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“…On the other hand, optical stimulation of neurite growth has also been proposed and demonstrated for many years. Neurite guidance is often conducted with a focused near-infrared-light spot or line ahead of the neurite tips or growth cones of axons 12,14,15,20 . Although these works are important for neural cell biology, it can be difficult to locate neurite tips or growth cones in living tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Among various physical factors that could influence neurite growth, light illumination is of interest because it can be introduced to the damaged neural tissues in a flexible and less intrusive manner. A number of optical stimulation techniques have been proposed for attracting or guiding growing neurites with high spatial accuracy and effectiveness 12–20 . On the other hand, blue light (wavelength: 400–500 nm, intensity: 0.1–10 mW/mm 2 ) induces the production of reactive oxygen species in cells and causes detrimental effects 21,22 or suppresses cell proliferation 23,24 .…”

Section: Introductionmentioning

confidence: 99%

Neurite regrowth stimulation by a red-light spot focused on the neuronal cell soma following blue light-induced retraction

Kao

Liao²,

Cheng

et al. 2019

Sci Rep

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The interaction of light with biological tissues has been considered for various therapeutic applications. Light-induced neurite growth has the potential to be a clinically useful technique for neuron repair. However, most previous studies used either a large illumination area to accelerate overall neurite growth or employed a light spot to guide a growing neurite. It is not clear if optical stimulation can induce the regrowth of a retracted neurite. In the present work, we used blue light (wavelength: 473 nm) to cause neurite retraction, and we proved that using a red-light (wavelength: 650 nm) spot to illuminate the soma near the junction of the retracted neurite could induce neurite regrowth. As a comparison, we found that green light (wavelength 550 nm) had a 62% probability of inducing neurite regrowth, while red light had a 75% probability of inducing neurite regrowth at the same power level. Furthermore, the neurite regrowth length induced by red light was increased by the pre-treatment with inhibitors of myosin functions. We also observed actin propagation from the soma to the tip of the re-growing neurite following red-light stimulation of the soma. The red light-induced extension and regrowth were abrogated in the calcium-free medium. These results suggest that illumination with a red-light spot on the soma may trigger the regrowth of a neurite after the retraction caused by blue-light illumination.

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Engineering Neuronal Patterning and Defined Axonal Elongation In Vitro

Bowser

Moore

2016

Neural Engineering

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Loop formation and self-fasciculation of cortical axon using photonic guidance at long working distance

Cited by 9 publications

References 48 publications

Spatial temperature gradients guide axonal outgrowth

Spatial temperature gradients guide axonal outgrowth

Neurite regrowth stimulation by a red-light spot focused on the neuronal cell soma following blue light-induced retraction

Engineering Neuronal Patterning and Defined Axonal Elongation In Vitro

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