Looking for new strategies to fight against mosquito-borne diseases: toward the development of natural extracts for mosquito control and reduction of mosquito vector competence

Bueno-Marí, Rubén

doi:10.3389/fphys.2013.00020

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…The opportunity to maintain resident adipose tissue macrophage and mast cell populations within organoid cultures offers the ability to better understand the role of the immune system in adipose tissue biology. Since inflammation is mainly responsible for obesity-associated complications such as diabetes as well as obesity-associated cancer development 5 , 6 , this model proves to be a useful tool to study various adipose tissue related diseases.…”

Section: Discussionmentioning

confidence: 99%

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Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism

Taylor

Sellin

Kuerschner

et al. 2020

Sci Rep

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Adipose tissue is an organized endocrine organ with important metabolic and immunological functions and immune cell-adipocyte crosstalk is known to drive various disease pathologies. Suitable 3D adipose tissue organoid models often lack resident immune cell populations and therefore require the addition of immune cells isolated from other organs. We have created the first 3D adipose tissue organoid model which could contain and maintain resident immune cell populations of the stromal vascular fraction (SVF) and proved to be effective in studying adipose tissue biology in a convenient manner. Macrophage and mast cell populations were successfully confirmed within our organoid model and were maintained in culture without the addition of growth factors. We demonstrated the suitability of our model for monitoring the lipidome during adipocyte differentiation in vitro and confirmed that this model reflects the physiological lipidome better than standard 2D cultures. In addition, we applied mass spectrometry-based lipidomics to track lipidomic changes in the lipidome upon dietary and immunomodulatory interventions. We conclude that this model represents a valuable tool for immune-metabolic research.

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Section: Discussionmentioning

confidence: 99%

“…These metabolic disorders are associated with adipose tissue dysfunction 33 . In addition, obesity has been correlated with the risk of various cancer types 5 . Adipose tissue responds to insulin by inhibiting lipolysis and increasing lipid storage leading to adipocyte expansion.…”

Section: Introductionmentioning

confidence: 99%

Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism

Taylor

Sellin

Kuerschner

et al. 2020

Sci Rep

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The repellency and toxicity effects of essential oils from the Libyan plants Salvadora persica and Rosmarinus officinalis against nymphs of Ixodes ricinus

et al. 2019

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Essential oils extracted from the leaves of Libyan Rosemary (Rosmarinus officinalis L.), and Miswak (Salvadora persica L.) were evaluated for their acaricidal and repellent effects on Ixodes ricinus L. nymphs (Acari: Ixodidae) using a bioassay based on an 'open filter paper method'. Rosmarinus officinalis leaf essential oil diluted to 0.5 and 1 µl/cm 2 in acetone exhibited, respectively, 20 and 100% tick mortality after about 5 h of exposure. A total of 50 and 95% of I. ricinus nymphs were killed by direct contact with the oil when exposed to lethal concentrations (LC) of 0.7 µl/cm 2 (LC 50) and 0.95 µl/cm 2 (LC 95), respectively. The LC 50 (0.5 µl/cm 2) was reached before the end of the first 24 h of exposure time (ET), as tick mortality at 24 h was 60%. Salvadora persica leaf essential oil at 1 µl/cm 2 showed a significant repellency effect against I. ricinus nymphs at 1.5 h ET. A 95% repellency was observed at a repellent concentration (RC 95) of 1 µl/cm 2 of S. persica, but no significant mortality was recorded at this dose of S. persica oil. Gas chromatography-mass spectrometry analyses showed that the main monoterpenes in both oils were 1,8-cineol, α-pinene, and β-pinene, although in markedly different proportions. These results suggest that essential oils have substantial potential as alternative approaches for I. ricinus tick control.

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Synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against Aedes aegypti L. (Diptera: Culicidae)

et al. 2018

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BackgroundIn a previous screening program for mosquitocides from local edible plants in Thailand, essential oils (EOs) of Cyperus rotundus, Alpinia galanga and Cinnamomum verum, were found to possess promising adulticidal activity against Aedes aegypti. With the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of EOs on permethrin toxicity against Ae. aegypti, both pyrethroid-resistant and -susceptible strains.MethodsEOs extracted from rhizomes of C. rotundus and A. galanga as well as C. verum barks were evaluated for chemical compositions and adulticidal activity against Muang Chiang Mai-susceptible (MCM-S) and Pang Mai Dang-resistant (PMD-R) strains of Ae. aegypti. Adulticidal bioassays of EO-permethrin mixtures for synergistic activity were also performed on these Ae. aegypti strains.ResultsChemical characterization by the GC-MS analytical technique demonstrated that 48 compounds were identified from the EOs of C. rotundus, A. galanga and C. verum, representing 80.22%, 86.75% and 97.24%, respectively, of all compositions. Cyperene (14.04%), β-bisabolene (18.27%) and cinnamaldehyde (64.66%) were the main constituents of C. rotundus, A. galanga and C. verum oils, respectively. In adulticidal bioassays, EOs of C. rotundus, A. galanga and C. verum were effective in killing Ae. aegypti, both MCM-S and PMD-R strains, with LD50 values of 10.05 and 9.57 μg/mg female, 7.97 and 7.94 μg/mg female, and 3.30 and 3.22 μg/mg female, respectively. The adulticidal efficacy against MCM-S and PMD-R Ae. aegypti of these EOs was close to that of piperonyl butoxide (PBO, LD50 values = 6.30 and 4.79 μg/mg female, respectively) but less pronounced than that of permethrin (LD50 values = 0.44 and 3.70 ng/mg female, respectively). Nevertheless, combination-based bioassays discovered the accomplished synergism of EOs together with permethrin. Significant synergistic effects with permethrin against both the strains of Ae. aegypti were recorded in the EOs of C. rotundus and A. galanga. Addition of C. rotundus and A. galanga oils decreased the LD50 values of permethrin against MCM-S dramatically from 0.44 to 0.07 and 0.11 ng/mg female, respectively, with synergism ratio (SR) values of 6.28 and 4.00, respectively. Furthermore, EOs of C. rotundus and A. galanga also reduced the LD50 values of permethrin against PMD-R drastically from 3.70 to 0.42 and 0.003 ng/mg female, respectively, with SR values of 8.81 and 1233.33, respectively.ConclusionsThe synergy of enhanced adulticidal toxicity recorded from EO-permethrin combinations against both strains of Ae. aegypti presents a promising role of EOs as a synergist for improving mosquitocidal efficacy, particularly in situations where conventional compounds are ineffective or inappropriate.

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Looking for new strategies to fight against mosquito-borne diseases: toward the development of natural extracts for mosquito control and reduction of mosquito vector competence

Cited by 3 publications

References 12 publications

Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism

Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism

The repellency and toxicity effects of essential oils from the Libyan plants Salvadora persica and Rosmarinus officinalis against nymphs of Ixodes ricinus

Synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against Aedes aegypti L. (Diptera: Culicidae)

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