Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays

Pelt, Jaap van; Corner, M.A.; Wolters, P.S.; Rutten, Wim; Ramakers, G.J.A.

doi:10.1016/j.neulet.2003.12.062

Cited by 166 publications

(142 citation statements)

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“…We studied NB profiles, which were calculated as a (smoothed) estimation of the array-wide firing frequency (Van Pelt et al 2004b;Stegenga et al 2008), without attempting to analyze neuron spiking behavior or explicit comparison with individual neurons in culture.…”

Section: Output Parametersmentioning

confidence: 99%

“…The most striking one is the phenomenon of more or less regular network bursts (NBs), i.e., almost simultaneous firing of many neurons in a relatively short time window. These spontaneous NBs have been subject of many experimental studies; see for example (Kamioka et al 1996;Jimbo et al 2000;Van Pelt et al 2004b;Stegenga et al 2008). Statistics of recorded bursting events are usually provided by averaging of the intra-burst firing rate profiles over long-term recordings.…”

Section: Introductionmentioning

confidence: 99%

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Network bursts in cortical cultures are best simulated using pacemaker neurons and adaptive synapses

et al. 2010

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One of the most specific and exhibited features in the electrical activity of dissociated cultured neural networks (NNs) is the phenomenon of synchronized bursts, whose profiles vary widely in shape, width and firing rate. On the way to understanding the organization and behavior of biological NNs, we reproduced those features with random connectivity network models with 5,000 neurons. While the common approach to induce bursting behavior in neuronal network models is noise injection, there is experimental evidence suggesting the existence of pacemaker-like neurons. In our simulations noise did evoke bursts, but with an unrealistically gentle rising slope. We show that a small subset of 'pacemaker' neurons can trigger bursts with a more realistic profile. We found that adding pacemaker-like neurons as well as adaptive synapses yield burst features (shape, width, and height of the main phase) in the same ranges as obtained experimentally. Finally, we demonstrate how changes in network connectivity, transmission delays, and excitatory fraction influence network burst features quantitatively.

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Section: Output Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Network bursts in cortical cultures are best simulated using pacemaker neurons and adaptive synapses

et al. 2010

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“…In the cortex, this hallmark of spontaneous activity has been reported since the earliest culture success in vitro (Crain, 1966;Calvet, 1974) and naturally occurs in all isolated cortex preparations thus studied, e.g. in organotypic cultures (Plenz and Aertsen, 1996a;Plenz and Aertsen, 1996b;Gorba, Klostermann et al, 1999;Klostermann and Wahle, 1999;Baker, Corner et al, 2006), dissociated cultures (Maeda, Robinson et al, 1995;Gopal and Gross, 1996;Kamioka, Maeda et al, 1996;Canepari, Bove et al, 1997;Jimbo, Kawana et al, 2000;Segev, Shapira et al, 2001;van Pelt, Corner et al, 2004;Eytan and Marom, 2006), acute cortex slices (Sanchez-Vives and McCormick, 2000), and acutely isolated cortical slabs in vivo (Steriade, Nuñez et al, 1993;Timofeev, Grenier et al, 2000). Quantification of these Correspondence: Dr. Dietmar Plenz, Section on Critical Brain Dynamics, National Institute of Mental Health, Porter Neuroscience Research Center, Bldg.…”

Section: Introductionmentioning

confidence: 99%

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Stewart

Plenz

2008

Journal of Neuroscience Methods

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Cortical networks in vivo and in vitro are spontaneously active in the absence of inputs, generating highly variable bursts of neuronal activity separated by up to seconds of quiescence. Previous measurements in adult rat cortex revealed an intriguing underlying organization of these dynamics, termed neuronal avalanches, which is indicative of a critical network state. Here we demonstrate that neuronal avalanches persist throughout development in cortical slice cultures from newborn rats. More specifically, we find that in spite of large variations of average rate in activity, spontaneous bursts occur with power-law distributed sizes (exponent -1.5) and a critical branching parameter close to 1. Our findings suggest that cortical networks homeostatically regulate a critical state during postnatal maturation.

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“…Complex networks of neurons can be cultured in vitro and kept alive and physiologically active for years at a time. Multiple distinct spatiotemporal oscillatory patterns emerge spontaneously in these chronic neural cultures [160,161,166]. Populations of neurons attached to a multi-electrode array can be connected to tissue-engineered axons that are free to integrate with a host nerve stump upon transplantation [69,112].…”

Section: Ectopic Neural Modulesmentioning

confidence: 99%

Techniques and devices to restore cognition

Serruya

Kahana

2008

Behavioural Brain Research

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Executive planning, the ability to direct and sustain attention, language and several types of memory may be compromised by conditions such as stroke, traumatic brain injury, cancer, autism, cerebral palsy and Alzheimer's disease. No medical devices are currently available to help restore these cognitive functions. Recent findings about the neurophysiology of these conditions in humans coupled with progress in engineering devices to treat refractory neurological conditions imply that the time has arrived to consider the design and evaluation of a new class of devices. Like their neuromotor counterparts, neurocognitive prostheses might sense or modulate neural function in a non-invasive manner or by means of implanted electrodes. In order to paint a vision for future device development, it is essential to first review what can be achieved using behavioral and external modulatory techniques. While non-invasive approaches might strengthen a patient's remaining intact cognitive abilities, neurocognitive prosthetics comprised of direct brain-computer interfaces could in theory physically reconstitute and augment the substrate of cognition itself.

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Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays

Cited by 166 publications

References 4 publications

Network bursts in cortical cultures are best simulated using pacemaker neurons and adaptive synapses

Network bursts in cortical cultures are best simulated using pacemaker neurons and adaptive synapses

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Techniques and devices to restore cognition

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