Despite being validated in different populations to assess fear of COVID-19, the Fear of COVID-19 Scale (FCV-19S) has scatter validations in healthcare professionals, often with several limitations, especially in Spanish-speaking professionals. Our research aims to extend previous studies by: (i) using a large sample of Spanish nurses and physicians; (ii) incorporating longitudinal data; and (iii) using a covariance-based SEM methodology to test different factor structures. 686 Spanish healthcare professionals (M = 42.7 years; 80.5% women; 76.7% nurses) participated in 2021 (Time 1), of whom, 216 were reassessed one year later (Time 2). The results (S-Bχ2 = 69.134, df = 13, p < 0.001, CFI = 0.974, and SRMR ≤ 0.031) supported a two-factor structure with a factor of somatic reactions and another of emotional expressions of fear of COVID-19. The FCV-19S evidenced strong factorial measurement invariance regarding gender, professional category, age and professional experience and also showed significantly higher levels of fear of COVID-19 in women, nurses, and professionals under 40 years old. The internal consistency was high for the somatic factor (ω = 0.86; α = 0.85), the emotional factor (ω = 0.82; α = 0.82) and the overall scale (α = 0.89). The scale showed good convergent, divergent, and incremental validity with respect to psychological symptomatology, perceived health, burnout, and worry about contagion. Finally, the FCV-19S showed criterion validity regarding generalized anxiety disorder, burnout, and the risk of leaving the profession. The FCV-19S evidenced excellent psychometric properties in Spanish healthcare professionals and was predictive of different health outcomes one year after administration. Study implications and limitations are also discussed.