Longitudinal survey of Teschovirus A, Sapelovirus A, and Enterovirus G fecal excretion in suckling and weaned pigs

Leme, Raquel Arruda; Silva, Danilo; Lorenzetti, Elis; Moraes, Daniel A.; Alfieri, Alice Fernandes; Alfieri, Amauri Alcindo

doi:10.1007/s42770-018-0018-1

Cited by 5 publications

(1 citation statement)

References 37 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virus is transmitted by the faecal-oral route and large number of viral particles is excreted during Rotavirus infection. In contrast, other RNA viruses including Kobuvirus, Astrovirus, Sapovirus , Sapelovirus , Teschovirus, and Torovirus, have been detected in pig faeces but its role as causative agents of neonatal diarrhoea has not so far been fully elucidated [10–14]. For instance, porcine Kobuvirus ( KobuV ) has been found frequently (16–99%) in faecal samples of pigs worldwide [15].…”

Section: Introductionmentioning

confidence: 99%

High levels of unreported intraspecific diversity among RNA viruses in faeces of neonatal piglets with diarrhoea

et al. 2019

View full text Add to dashboard Cite

BackgroundDiarrhoea is a major cause of death in neonate pigs and most of the viruses that cause it are RNA viruses. Next Generation Sequencing (NGS) deeply characterize the genetic diversity among rapidly mutating virus populations at the interspecific as well as the intraspecific level. The diversity of RNA viruses present in faeces of neonatal piglets suffering from diarrhoea in 47 farms, plus 4 samples from non-diarrhoeic piglets has been evaluated by NGS. Samples were selected among the cases submitted to the Veterinary Diagnostic Laboratories of Infectious Diseases of the Universitat Autònoma de Barcelona (Barcelona, Spain) and Universidad de León (León, Spain).ResultsThe analyses identified the presence of 12 virus species corresponding to 8 genera of RNA viruses. Most samples were co-infected by several viruses. Kobuvirus and Rotavirus were more commonly reported, with Sapovirus, Astrovirus 3, 4 and 5, Enterovirus G, Porcine epidemic diarrhoea virus, Pasivirus and Posavirus being less frequently detected. Most sequences showed a low identity with the sequences deposited in GenBank, allowing us to propose several new VP4 and VP7 genotypes for Rotavirus B and Rotavirus C.ConclusionsAmong the cases analysed, Rotaviruses were the main aetiological agents of diarrhoea in neonate pigs. Besides, in a small number of cases Kobuvirus and Sapovirus may also have an aetiological role. Even most animals were co-infected in early life, the association with enteric disease among the other examined viruses was unclear. The NGS method applied successfully characterized the RNA virome present in faeces and detected a high level of unreported intraspecific diversity.

show abstract

Section: Introductionmentioning

confidence: 99%