Longitudinal Surveillance for SARS-CoV-2 Among Staff in Six Colorado Long Term Care Facilities: Epidemiologic, Virologic and Sequence Analysis

Gallichotte, Emily N.; Quicke, Kendra M.; Sexton, Nicole R.; Fitzmeyer, Emily; Janich, Ashley J; Dobos, Karen M.; Gahm, Gregory A.; Ebel, Gregory D.; Ehrhart, Nicole

doi:10.2139/ssrn.3724248

Cited by 8 publications

(10 citation statements)

References 40 publications

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“…32 Others have proposed cellular interactions between viral proteins and circadian-dependent host signals, 33 and demonstrated circadian-dependent entry and proliferation of SARS-CoV-2 in lung epithelial cell types in culture. 34 Regardless of mechanism, because higher viral loads are associated with replication-competent culturable virus, 35,36 these findings may also suggest a higher risk of transmission in the morning.…”

Section: Discussionmentioning

confidence: 99%

Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking

et al. 2022

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Our findings suggest that collecting saliva and nasal swab specimens in the morning immediately after waking yields higher SARS-CoV-2 viral loads than collection later in the day. The higher viral loads from morning specimen collection are predicted to significantly improve detection of SARS-CoV-2 in symptomatic individuals, particularly when using moderate- to low-analytical-sensitivity COVID-19 diagnostic tests, such as rapid antigen tests.

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Section: Discussionmentioning

confidence: 99%

Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking

et al. 2022

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“…Some clinical studies have shown that when Ct values are equal to or higher than 34 or the viral load drops below 10 3 copies μL −1 , the infectivity and the viral transmission risk is low [46] . Recent modeling of viral dynamics also indicated that a LOD of 10 2 copies μL −1 is qualified for screening of SARS‐CoV‐2 infection [14, 47, 48] . It is also worth noting that the test efficiency of Orf1ab gene is lower than that of E gene due to the competition of T1, T2 and C line for AuNP‐DNA probes, which may lead to false‐negative results.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Dual‐Gene Diagnosis of SARS‐CoV‐2 Based on CRISPR/Cas9‐Mediated Lateral Flow Assay

et al. 2021

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Fewm ethods for the detection of SARS-CoV-2 currently have the capability to simultaneously detect two genes in as ingle test, whichi sakey measure to improve detection accuracy,a sa dopted by the gold standardR T-qPCR method. Developed here is aCRISPR/Cas9-mediated triple-line lateral flowa ssay(TL-LFA) combined with multiplex reverse transcription-recombinase polymerase amplification (RT-RPA) for rapid and simultaneous dual-gene detection of SARS-CoV-2 in as ingle strip test. This assayi sc haracterized by the detection of envelope (E) and open reading frame 1ab (Orf1ab) genes from cell-cultured SARS-CoV-2 and SARS-CoV-2 viral RNAstandards,showing asensitivity of 100 RNA copies per reaction (25 mL). Furthermore,d ual-gene analysis of 64 nasopharyngeal swab samples showed 100 %n egative predictive agreement and 97.14 %p ositive predictive agreement. This platform will provide am ore accurate and convenient pathway for diagnosis of COVID-19 or other infectious diseases in low-resource regions.

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“…The RNA extracted from the FMS and potentially recovered live viruses will be used as basis for deep sequencing. 3 …”

Section: Methods and Analysismentioning

confidence: 99%

“…Asymptomatic SARS-CoV-2-infected individuals carry viral loads in conventional samples from the nasopharynx that are in the same range as those of symptomatic individuals, and a significant proportion of SARS-CoV-2 spread is due to asymptomatic individuals. [1][2][3][4][5][6] An analytical model published by the Centers for Disease Control and Prevention (CDC)…”

Section: Introductionmentioning

confidence: 99%

Investigating transmission of SARS-CoV-2 using novel face mask sampling: a protocol for an observational prospective study of index cases and their contacts in a congregate setting

et al. 2022

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IntroductionThis study aims to measure how transmission of SARS-CoV-2 occurs in communities and to identify conditions that lend to increased transmission focusing on congregate situations. We will measure SARS-CoV-2 in exhaled breath of asymptomatic and symptomatic persons using face mask sampling—a non-invasive method for SARS-CoV-2 detection in exhaled air. We aim to detect transmission clusters and identify risk factors for SARS-CoV-2 transmission in presymptomatic, asymptomatic and symptomatic individuals.Methods and analysisIn this observational prospective study with daily follow-up, index cases and their respective contacts are identified at each participating institution. Contact definitions are based on Centers for Disease Control and Prevention and local health department guidelines. Participants will wear masks with polyvinyl alcohol test strips adhered to the inside for 2 hours daily. The strips are applied to all masks used over at least 7 days. In addition, self-administered nasal swabs and (optional) finger prick blood samples are performed by participants. Samples are tested by standard PCR protocols and by novel antigen tests.Ethics and disseminationThis study was approved by the Colorado Multiple Institutional Review Board and the WHO Ethics Review Committee. From the data generated, we will analyse transmission clusters and risk factors for transmission of SARS-CoV-2 in congregate settings. The kinetics of asymptomatic transmission and the evaluation of non-invasive tools for detection of transmissibility are of crucial importance for the development of more targeted control interventions—and ultimately to assist with keeping congregate settings open that are essential for our social fabric.Trial registration numberClinicalTrials.gov (#NCT05145803).

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Longitudinal Surveillance for SARS-CoV-2 Among Staff in Six Colorado Long Term Care Facilities: Epidemiologic, Virologic and Sequence Analysis

Cited by 8 publications

References 40 publications

Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking

Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking

Simultaneous Dual‐Gene Diagnosis of SARS‐CoV‐2 Based on CRISPR/Cas9‐Mediated Lateral Flow Assay

Investigating transmission of SARS-CoV-2 using novel face mask sampling: a protocol for an observational prospective study of index cases and their contacts in a congregate setting

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