Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus–Infected Patients

Lum, Fok‐Moon; Lye, David C.; Tan, Jeslin J. L.; Lee, Bernett; Chia, Po Ying; Chua, Tze-Kwang; Amrun, Siti Naqiah; Kam, Yiu‐Wing; Yee, Wearn-Xin; Ling, Weiping; Lim, Vanessa; Pang, Junxiong; Lee, Linda K.; Mok, Esther Wing Hei; Chong, Chia-Yin; Leo, Yee‐Sin; Ng, Lisa F. P.

doi:10.1093/infdis/jiy225

Cited by 44 publications

(72 citation statements)

References 50 publications

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“…Specifically, IgG1 and IgG3 were the subclasses induced following ZIKV infection, closely resembling DENV-infected patients. 35 Moreover, none of the ZIKV patients in our study displayed severe symptoms to suggest occurrence of antibodydependent enhancement (ADE), 30 and similar observations were also reported from Brazil. This observation is also supported by another study, in which the profiles of ZIKV-neutralising antibodies of patients from Nicaragua, Sri Lanka and Thailand were not affected by previous DENV infection.…”

Section: Discussionsupporting

confidence: 86%

“…Anti-ZIKV IgM and IgG levels of ZIKV patients from the Singapore outbreak in 2016 [28][29][30] were longitudinally assessed using virion-based ELISA. 18,[24][25][26][27] The majority of the patients showed a robust ZIKVspecific humoral response (Figure 1a IgG isotypes produced by ZIKV patients were then determined, and highest titres of anti-ZIKV IgG1 and IgG3 subtypes were produced at early convalescent for IgG3 and late convalescent for IgG1 (Figure 1d).…”

Section: Zikv Patients Produce a Robust And Protective Humoral Responsementioning

confidence: 99%

“…30 Briefly, 65 patients who were RT-PCR-positive for ZIKV in whole blood or urine, and negative for DENV RT-PCR were enrolled. 30 Briefly, 65 patients who were RT-PCR-positive for ZIKV in whole blood or urine, and negative for DENV RT-PCR were enrolled.…”

Section: Singapore Zikv Patientsmentioning

confidence: 99%

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Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

et al. 2019

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Objectives Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology‐based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross‐reactive antibodies, which confounds serological interpretations. Methods Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non‐structural 1 (NS1) viral proteins in a peptide‐based ELISA for epitope identification. Identified epitopes were re‐validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. Results Long‐lasting ZIKV‐neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B‐cell epitopes were identified, and of these, four common flavivirus, three ZIKV‐specific and one DENV‐specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV‐specific peptide 26 on domain I/II of E protein (amino acid residues 271–288) presented 80% sensitivity and 85.7% specificity. Importantly, the differential epitopes also showed significance in differentiating non‐flavivirus patient samples. Conclusion Linear B‐cell epitope candidates to differentiate between ZIKV and DENV infections were identified, providing the first step towards the design of a much‐needed serology‐based assay.

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Section: Discussionsupporting

confidence: 86%

Section: Zikv Patients Produce a Robust And Protective Humoral Responsementioning

confidence: 99%

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Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

et al. 2019

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“…Nevertheless, a series of histologic, immunologic and transcriptomic analyses were used to determine the impact of ZIKV infection on pregnancy progression and the cellular responses of the host towards the infection. All three babies were born at term with no observable birth defects, and all of the ZIKV‐infected mothers were clinically well, as with majority of ZIKV‐infected patients . However, different degrees of infection were apparent in the level of CD14 + monocytes between the three patients.…”

Section: Discussionmentioning

confidence: 84%

“…As confirmed by MFA of immune‐phenotyping and cytokine data, the three pregnant patients can be easily separated based on their immune response. Importantly, by comparing their cytokine profiles to those of the healthy non‐pregnant female controls, several immune mediators such as IL‐1RA, EGF, RANTES and IP‐10 were associated with the acute phase of ZIKV infection, a finding that was also observed in ZIKV‐infected non‐pregnant patients . Interestingly, EGF and IP‐10 are typically expressed at higher levels in women carrying a foetus with developmental anomalies .…”

Section: Discussionmentioning

confidence: 85%

Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus‐infected women

et al. 2019

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Objectives Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods Full‐term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results In this study, each woman exhibited a unique immune response with raised IL‐1RA, IP‐10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co‐localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion These findings should translate to improve clinical prenatal screening procedures for virus‐infected pregnant patients.

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Interleukin‐17—A multifaceted cytokine in viral infections

Sahu

Biswas

Prajapati

et al. 2021

Journal Cellular Physiology

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Viral infections are a major threat to the human population due to the lack of selective therapeutic measures. The morbidity and mortality reported worldwide are very alarming against viral pathogens. The proinflammatory environment is required for viral inhibition by initiating the host immune response. The host immune response fights these pathogens by secreting different cytokines. Interleukin‐17 (IL‐17) a proinflammatory cytokine mainly produced by T helper type 17 cells, plays a vital role in the regulation of host immune response against various pathogens, including viruses. However, dysregulated production of IL‐17 induces chronic inflammation, autoimmune disorders, and may lead to cancer. Recent studies suggest that IL‐17 is not only involved in the antiviral immune response but also promotes virus‐mediated illnesses. In this review, we discuss the protective and pathogenic role of IL‐17 against various viral infections. A detailed understanding of IL‐17 during viral infections could contribute to improve therapeutic measures and enable the development of an efficient and safe IL‐17 based immunotherapy.

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Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus–Infected Patients

Cited by 44 publications

References 50 publications

Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus‐infected women

Interleukin‐17—A multifaceted cytokine in viral infections

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