Longitudinal shifts in bacterial diversity and fermentation pattern in the rumen of steers grazing wheat pasture

Pitta, Dipti; Pinchak, W. E.; Dowd, Scot E.; Dorton, K. L.; Yoon, I.; Min, Byeng R.; Fulford, J. D.; Wickersham, T.A.; Malinowski, Dariusz P.

doi:10.1016/j.anaerobe.2014.07.008

Cited by 41 publications

(33 citation statements)

References 44 publications

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“…Regardless of treatment, a total of 19 phyla were found in this study, and Bacteroidetes and Firmicutes were the two predominant phyla and the sum of these two phyla accounted for 90% of the micro‐organisms in the rumen samples in this study. This was in agreement with previous studies in other ruminants (Sundset et al., ; Ley et al., ; Kong et al., ; Jami et al., ; Pitta et al., ). However, there were only three phyla (Proteobacteria, Planctomycetes and Chloroflexi) which were significantly different (p < 0.05, Table ) in relative abundance between the two groups.…”

Section: Discussionsupporting

confidence: 94%

The impact of rumen cannulation on the microbial community of goat rumens as measured using 16S rRNA high‐throughput sequencing

Yan

Wang

2017

Animal Physiology Nutrition

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SummaryThe main objective of this study was to determine the impact of rumen cannulation on the microbial community of goat rumens using 16S rRNA high-throughput sequencing. Twelve Boer crossbred goats were used in the experiment: six goats were surgically fitted with rumen cannula, and the other six were used as controls. All goats were fed the same diet for 20 days, after which their rumen digesta were sampled once per week for three consecutive weeks. Total microbial DNA was extracted from the collected rumen fluid and was used as a template to amplify the V4 hypervariable region of the 16S rRNA gene. High-throughput sequencing was performed using an Illumina MiSeq platform, and the sequences were analyzed primarily using the Quantitative Insights into Microbial Ecology pipeline software. The results showed that the Chao 1 index, the observed species index and the Shannon-Wiener index were not significantly different (p > 0.05) between the two groups. Bacteroidetes, Firmicutes, Tenericutes were the predominant phylum in both groups, and their relative abundance was 60.63%, 29.48%, 2.24% (n = 6, CT group) and 61.17%, 26.92%, 1.66% (n = 6, RC group) respectively. At the phylum level, the relative abundance of Proteobacteria was significantly higher (p < 0.001) in the microbial communities of RC goats, and Planctomycetes and Chloroflexi were significantly lower (p = 0.02). The abundances of other phyla were not significantly different between treatments. A total of 19 lower-level taxa also exhibited significant differences (p < 0.05) in relative abundance between the groups. In addition, there were 18 genera shared within the control group, 26 shared within the rumen-cannulated group, and 16 shared by both groups. Prevotella was the most abundant shared genus, although its abundance was not significantly different (p > 0.05) between the groups. In conclusion, although the most abundant microbes kept stable, rumen cannulation had the potential to significantly change rumen microbial communities in goats.

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Section: Discussionsupporting

confidence: 94%

The impact of rumen cannulation on the microbial community of goat rumens as measured using 16S rRNA high‐throughput sequencing

Yan

Wang

2017

Animal Physiology Nutrition

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“…The 10 bacterial genera whose relative abundance was affected by three‐way interaction (treatment, diet and time) contributed to less than 3% of the total bacterial community. Among them, Lachnoclostridium (Ravachol et al , 2015), Moryella (Pitta et al , 2014) and Papillibacter (Zhang et al , 2014) were reported to be associated with the degradation of polysaccharide in the rumen. Interestingly, the relative abundance of the three genera was affected by the three‐way interaction in a similar pattern.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol affects in vitro rumen fermentation, methane production and prokaryotic community composition in a time‐ and diet‐specific manner

Yan

et al. 2020

Microbial Biotechnology

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This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets (P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter (P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet (P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time (P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales. Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.

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“…The different treatments did not affect the relative abundance of Prevotella , similar to the findings of a previous study 60 . Prevotella is a predominant organism in bulls fed both forage and grain 48 and contributes to the majority of hereditary and metabolic variety of the microflora 61 .…”

Section: Discussionmentioning

confidence: 99%

Effects of replacing Leymus chinensis with whole-crop wheat hay on Holstein bull apparent digestibility, plasma parameters, rumen fermentation, and microbiota

Niu

Xia

et al. 2017

Sci Rep

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Twelve Holstein bulls were used in a 4 × 4 Latin square design to investigate the effects of using whole-crop wheat hay (WCWH) as a substitute for Leymus chinensis (LC) on apparent digestibility, plasma parameters, ruminal fermentation, and microbial communities. Experimental treatments were four proportions of WCWH, 0, 33, 67, and 100%, as a substitute for LC (WCWH0, WCWH33, WCWH67, and WCWH100, respectively). The WCWH100 group showed a higher nutritional intake of crude protein (CP) and higher apparent digestibility of organic matter (OM), CP, and ether extract (EE) than the WCWH0 group (P < 0.05). Urea N, NH3-N, isobutyrate and isovalerate levels were higher (P < 0.05) in the WCWH100 group than in the WCWH0 group. 16S rRNA high-throughput sequencing analysis revealed similarities in the community composition, species diversity and relative abundance of dominant bacteria at the phylum and genus levels among the four groups. Collectively, our data indicated that WCWH can be used to replace LC in the diet of finishing dairy bulls without having a negative impact on apparent digestibility, plasma parameters, and ruminal bacteria composition. These results offer the first deep insight into the effects of replacing LC with WCWH on the performance parameters and rumen microbiota in Holstein bulls, and may aid in ruminant farming.

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Longitudinal shifts in bacterial diversity and fermentation pattern in the rumen of steers grazing wheat pasture

Cited by 41 publications

References 44 publications

The impact of rumen cannulation on the microbial community of goat rumens as measured using 16S rRNA high‐throughput sequencing

The impact of rumen cannulation on the microbial community of goat rumens as measured using 16S rRNA high‐throughput sequencing

Resveratrol affects in vitro rumen fermentation, methane production and prokaryotic community composition in a time‐ and diet‐specific manner

Effects of replacing Leymus chinensis with whole-crop wheat hay on Holstein bull apparent digestibility, plasma parameters, rumen fermentation, and microbiota

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