Longitudinal screening of antibiotic residues, antibiotic resistance genes and zoonotic bacteria in soils fertilized with pig manure

Meersche, Tina Van den; Rasschaert, Geertrui; Nest, Thijs Vanden; Haesebrouck, Freddy; Herman, Lieve; Coillie, Els Van; Weyenberg, Stéphanie Van; Daeseleire, Els; Heyndrickx, Marc

doi:10.1007/s11356-020-09119-y

Cited by 34 publications

(16 citation statements)

References 47 publications

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“…However, ARGs can also be present in healthy animals, regardless of their prior antimicrobial exposure [ 11 ]. Manure from animals that have not received antibiotics may also contain high numbers of ARGs [ 12 ], and ARGs can persist on farms for many years after the abolishment of antibiotic use [ 13 ]. These observations suggest that other factors such as historical antimicrobial use and farm management practices should also be considered to explain variations in antimicrobial resistance (AMR) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

et al. 2022

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The aim of this study was metagenomics analyses of acquired antibiotic-resistance genes (ARGs) in the intestinal microbiome of two important food-animal species in Hungary from a One Health perspective. Intestinal content samples were collected from 12 domestic pigs (Sus scrofa) and from a common carp (Cyprinus carpio). Shotgun metagenomic sequencing of DNA purified from the intestinal samples was performed on the Illumina platform. The ResFinder database was applied for detecting acquired ARGs in the assembled metagenomic contigs. Altogether, 59 acquired ARG types were identified, 51 genes from domestic pig and 12 genes from the carp intestinal microbiome. ARG types belonged to the antibiotic classes aminoglycosides (27.1%), tetracyclines (25.4%), β-lactams (16.9%), and others. Of the identified ARGs, tet(E), a blaOXA-48-like β-lactamase gene, as well as cphA4, ampS, aadA2, qnrS2, and sul1, were identified only in carp but not in swine samples. Several of the detected acquired ARGs have not yet been described from food animals in Hungary. The tet(Q), tet(W), tet(O), and mef(A) genes detected in the intestinal microbiome of domestic pigs had also been identified from free-living wild boars in Hungary, suggesting a possible relationship between the occurrence of acquired ARGs in domestic and wild animal populations.

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Section: Introductionmentioning

confidence: 99%

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

et al. 2022

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“…Inappropriate use of antibiotics in humans (in hospitals, communities) and animals (including livestock, poultry, aquaculture) and the utilization of animal waste from antibiotic fed animals, like poultry as manure or fertilizer in fields, also act as a source of introducing antibiotics and antibiotic-resistant genes (ARGs) in the soil and water bodies (Meersche et al 2020) which are part of the environment. The evident sources contributing to AMR's environmental role include agricultural and poultry farms, effluents from pharmaceutical industries and healthcare facilities, and expired or unused antibiotics disposed of by household and community (Khurana and Sinha 2019).There are numerous scientific studies that demonstrate the presence of antibiotic-resistant bacteria and the presence of antibiotic residues and resistance genes in the environment (Kumar and Kotwani 2017;Tamhankar and Lundborg 2019;Singer et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Pharmaceutical effluent: a critical link in the interconnected ecosystem promoting antimicrobial resistance

Kotwani

Joshi

Kaloni

2021

Environ Sci Pollut Res

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Antimicrobial resistance (AMR) is a complex global health issue and will push twenty-four million people into extreme poverty by 2030, risking the sustainable development goals (SDGs) 2, 3, 6, 9, 12, and 17 if not addressed immediately. Humans, animals, and the environment are the reservoirs that contribute and allow AMR to propagate in interconnected ecosystems. The emergence of antibiotic-resistant bacteria and antibiotic-resistant genes in the water environment has become an important environmental health issue. One of the major influencers from environment sector is the pharmaceutical industry which is growing globally to meet the ever-increasing demand of antibiotics, especially in low-and middle-income countries. The pharmaceutical effluent has a mix of large concentrations of antibiotics and antibiotic resistance genes, and these sites act as hotspots for environmental contamination and the spread of AMR. Inadequate treatment of the effluent and its irresponsible disposal leads to unprecedented antibiotic contamination in the environment and their persistent presence in the environment significantly modulates the bacterial genomes' expression that is responsible for increase and spread of AMR. However, not much interventions are suggested in the National Action Plan developed on AMR by many countries. There are no regulations across the globe till date for the level of antibiotic residues in pharmaceutical effluent for the growing pharmaceutical industry. This review put together the work done showing several detrimental effects of the antimicrobial residues in the pharmaceutical effluent which leads to rise in development of AMR. The environment risk approach and need to have indicators to measure environment risk is a way forward for all countries engage in antibiotic manufacturing. Overall, efforts to address the problem are isolated and fragmented. Policymakers, regulators, manufacturers, researchers, civil society, and the community need to collaborate so that antibiotics are produced sustainably and continue to stay effective in treating bacterial infections.

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“…The level of resistance to antibacterials by normal and pathogenic strains was increasing at an alarming rate for several decades (Danner et al 2019). Since then, the uncontrolled use of pharmaceutical substances in industry, hospital, agriculture, and aquaculture has introduced several antibacterial to the aquatic environment (Bengtsson-Palme &Larsson 2016, Ryu et al 2019, Van den Meersche et al 2020). The abuse of antibacterials imposes new evolutionary pressure on non-pathogenic and pathogenic bacterial strains (Kummerer 2009b, a).…”

Section: Introductionmentioning

confidence: 99%

Riverine antibacterial resistance gradient determined by environmental factors

Liu

Chuang

Gurunathan

et al. 2021

Preprint

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Wastewater emission to surface waters is a major pathway for antibacterials and antibacterial-resistant bacteria. Polluted waterbodies such as rivers provide a reservoir for bacterial resistance. We studied water quality and bacterial antibacterial resistance along the subtropical Qishan River in Taiwan as a case study of environmental resistance spread in a pristine to rural area. Human settlement densities increased generally from pristine mountain sites to the more polluted lowlands generally. Accordingly, as a working hypothesis, we expected antibacterial resistance level to increase towards downstream. We collected sediment samples from 8 stations along the Qishan river and where the Qishan river reaches the Kaoping river. The samples were processed in the lab for bacteriological and physicochemical analysis. Antibacterial resistance was tested by disk diffusion and micro-dilution with ten common antibacterials. A comparison was made among the sites where isolates began to occur at the upstream (site 1-6) with the downstream, including site 7 (Qishan town), site 8 (wastewater treatment plant) and site 9 (Kaoping river). The results of multivariate analysis for bacteriological and physicochemical parameters showed increasing water pollution levels downstream of the Qishan river. Ten bacteria including Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Enterobacter sp., Acinetobacter sp., Staphylococcus spp. and Bacillus spp. were analyzed and tested in the study. Their percentage of occurrence varied at each site. The resistance level was determined from the growth inhibition zone diameter (disk diffusion) and the minimum inhibitory concentration (micro-dilution). The results indicated that antibacterial resistance was related to certain environmental factors. Besides, the usage pattern of different classes of antibacterials in different places could alter trends of their resistance. Bacteria were found with increased resistance to antibacterials used in agriculture through the downstream sites according to the results. The WWTP emitting wastewater was demonstrated to be a hotspot of resistance in aquatic environments. In conclusion, bacterial resistance against antibacterials from the Qishan river has become a potential public health threat. This study could assist authorities by providing a reference for water quality risk assessment and management in Kaohsiung city and southern Taiwan.

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Longitudinal screening of antibiotic residues, antibiotic resistance genes and zoonotic bacteria in soils fertilized with pig manure

Cited by 34 publications

References 47 publications

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

Pharmaceutical effluent: a critical link in the interconnected ecosystem promoting antimicrobial resistance

Riverine antibacterial resistance gradient determined by environmental factors

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