Longitudinal proteomics study of serum changes after allogeneic HSCT reveals potential markers of metabolic complications related to aGvHD

Abstract

Even though hematopoietic stem cell transplantation (HSCT) allows successful treatment for many malignant and non-malignant disorders, its curative potential remains limited by severe side effects, including infections and other transplant-related complications such as graft-versus-host disease (GvHD). This study examined changes in serum proteome via high-performance two-dimensional gel electrophoresis (2-DE) during HSCT to search for diagnostic biomarkers for post-HSCT complications. Longitudinal proteomic analysis revealed proteins related to metabolic complications and hemolytic anemia. Retinol-binding protein 4 (RBP4), a reliable marker of insulin resistance, was identified, and is possibly associated with the onset mechanism of acute graft-versus-host disease (aGvHD) and/or skin GvHD. Although the cause of insulin resistance is not fully understood, it is thought to be associated with adipocytes inflammation induced by RBP4, iron overload and hemolytic anemia after HSCT, as observed in this study. The present study has demonstrated that insulin resistance and metabolic complications could be immediate complications after transplantation and are associated with aGvHD. The biomarkers revealed in this study are promising tools to be used for improving the early diagnosis of HSCT-associated complications, especially aGvHD, possibly even before clinical manifestations.

Proteomics of appetite-regulating system influenced by menstrual cycle and intensive exercise in female athletes: a pilot study

“…Pretreatment of serum for the removal of major proteins that would otherwise impede the analysis of minor proteins in 2DE was performed following previously described methods 24 , 25 . Briefly, 60 µL of serum was mixed with 180 µL of dilution buffer (Seppro IgY 14 protein kit, Sigma-Aldrich Co., St. Louis, MO, US) and filtered through a Spin-X Column (0.45 µm, Corning, New York, U.S.) at 10,000 rpm.…”

“…The 2DE experiment was conducted following previously reported methods 24 , 25 . Briefly, the DeStreak Rehydration Solution containing 50 µg of proteins was absorbed into Immobiline DryStrips (7 cm, pH 3–10, Cytiva, MA, US) for 18 h at room temperature.…”

“…Omics approaches emerged in the 2000s and currently contribute to various research areas. Proteomics is a widely-used approach for elucidating complicated protein functions, and 2D gel electrophoresis has been advantageous for detecting natural-form proteins, especially when targeting post-translational modifications 23 – 25 .…”

The effect of transfusion of erythrocyte mass on clinical and haematological indicators of dogs with hemolytic anaemia caused by babesiosis

“…S.Y. Wong et al (2022) found in their research that the use of red blood cells reduces the number of microaggregates, the absence of plasma coagulation factors and a significant number of platelets, and reduces the risk of contracting vector-borne infections.…”

“…S.Y. Wong et al (2022) note that transfusion of blood and its components is effective in animals with anaemia of various genesis (post-haemorrhagic, haemolytic, aplastic). B.…”