Longitudinal micro-computed tomography monitoring of progressive liver regeneration in a mouse model of partial hepatectomy

Will, Olga; Damm, Timo; Campbell, Graeme; Schönfells, Witigo von; Açil, Yahya; Will, Marcus; Chalaris-Rissmann, Athena; Ayna, Mustafa; Drucker, Claudia; Glüer, Claus-Christian

doi:10.1177/0023677216678824

Cited by 9 publications

(9 citation statements)

References 7 publications

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“…Consequently, both spleen and liver are highly contrasted by micro-CT imaging. Anatomical evaluations of the spleen and the liver in normal and regenerative processes using ExiTron nano 12000 have been reported (Das et al., 2016, Will et al., 2017). Importantly, ExiTron nano 12000 does not cause hepatotoxicity and does not lead to pro-inflammatory cytokine release in the liver or serum, thus ensuring a relatively safe approach for longitudinal pharmacology and toxicology studies (Boll et al., 2011, Boll et al., 2013, Liu et al., 2019, Mannheim et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

et al. 2019

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SummaryComputed tomography is a powerful medical imaging modality for longitudinal studies in cancer to follow neoplasia progression and evaluate anticancer therapies. Here, we report the generation of a photon-counting micro-computed tomography (PC-CT) method based on hybrid pixel detectors with enhanced sensitivity and precision of tumor imaging. We then applied PC-CT for longitudinal imaging in a clinically relevant liver cancer model, the Alb-R26Met mice, and found a remarkable heterogeneity in the dynamics for tumors at the initiation phases. Instead, the growth curve of evolving tumors exhibited a comparable exponential growth, with a constant doubling time. Furthermore, longitudinal PC-CT imaging in mice treated with a combination of MEK and BCL-XL inhibitors revealed a drastic tumor regression accompanied by a striking remodeling of macrophages in the tumor microenvironment. Thus, PC-CT is a powerful system to detect cancer initiation and progression, and to monitor its evolution during treatment.

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Section: Introductionmentioning

confidence: 99%

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

et al. 2019

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“…To provide a reference standard, we also quantitatively measured the remnant liver volume via contrast-enhanced μCT, which has been commonly used in clinical settings for hepatic diagnosis 33 . The μCT analysis revealed that liver volume was significantly increased after resection, up to 191.0% ± 35.0% and 218.3% ± 53.8% at day 5 and day 7, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

et al. 2022

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High spatial resolution, low background, and deep tissue penetration have made near-infrared II (NIR-II) fluorescence imaging one of the most critical tools for in vivo observation and measurement. However, the relatively short retention time and potential toxicity of synthetic NIR-II fluorophores limit their long-term application. Here, we report the use of infrared fluorescent proteins (iRFPs) as in vitro and in vivo NIR-II probes permitting prolonged continuous imaging (up to 15 months). As a representative example, iRFP713 is knocked into the mouse genome to generate a transgenic model to allow temporal and/or spatial expression control of the probe. To demonstrate its feasibility in a genuine diagnostic context, we adopt two liver regeneration models and successfully track the process for a week. The performance and monitoring efficacy are comparable to those of μCT and superior to those of indocyanine green dye. We are also able to effectively observe the pancreas, despite its deep location, under both physiological and pathological conditions. These results indicate that the iRFP-assisted NIR-II fluorescence system is suitable for monitoring various tissues and in vivo biological processes, providing a powerful noninvasive long-term imaging platform.

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“…In clinical practice, the linear dimensions, volume, and structure of the liver are examined using traditional imaging techniques such as ultrasound, CT, MRI, and ultrasound elastography. In suspected pathology, the liver parenchyma is examined for its regenerative potential and the risk of liver failure after surgery [17,18].…”

Section: Traditional Methods For Assessing the Linear Dimensions Volmentioning

confidence: 99%

“…Fluorescence microscopy allows evaluating the presence and distribution of specific molecules and investigating cellular events in real time. Recent data obtained with fluorescence imaging provide some insight into the structure and function of normal and pathological liver [17]. For example, the microstructure of hepatocytes was studied using confocal single-photon [94] and multiphoton microscopy (MPM) [95,96].…”

Section: Promising Methods For Assessing the Structure Function Andmentioning

confidence: 99%

Modern Methods for Assessing the Regenerative Potential of the Liver after Partial Hepatectomy (Review)

Rodimova¹,

Kuznetsova²,

Bobrov³

et al. 2019

Sovrem Tehnol Med

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The review addresses the main methods for assessing the function and regenerative potential of the liver. They include both the traditional methods, commonly used in clinical practice, and the latest promising techniques suitable for the analysis of cellular and tissue pathology and having a proven diagnostic value.It is known that the dynamics of liver regeneration is reflected in the metabolic status of liver cells, their morphology, and the molecular rearrangement. Therefore, by looking at these parameters we will be able to assess the regenerative potential of the liver as a whole.At present, the most promising method is represented by multiphoton microscopy able to generate the second harmonic; there are also such techniques as coherent anti-stokes Raman spectroscopy (CARS), stimulated Raman scattering (SRS) microscopy, and fluorescence lifetime imaging microscopy (FLIM). In addition, a number of options for analyzing metabolic and structural changes are provided by mass spectrometry, in particular time-of-flight secondary ion mass spectrometry (ToF-SIMS). Studies using these methods with in vivo models and with human biopsy samples demonstrate their relevance in biomedical research and in clinical practice alike.

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Longitudinal micro-computed tomography monitoring of progressive liver regeneration in a mouse model of partial hepatectomy

Cited by 9 publications

References 7 publications

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography

Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

Modern Methods for Assessing the Regenerative Potential of the Liver after Partial Hepatectomy (Review)

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