Longitudinal investigation of the faecal microbiota of healthy full-term infants using fluorescence in situ hybridization and denaturing gradient gel electrophoresis

Roger, Laure C.; McCartney, Anne L.

doi:10.1099/mic.0.041913-0

Cited by 96 publications

(98 citation statements)

References 36 publications

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“…Culture-based and current large-scale metagenomic studies show that Bifidobacterium is a dominant genus in the intestinal microbiota of breast-fed infants, in some cases representing approximately 75 % of total bacteria (Harmsen et al, 2000;Roger & McCartney, 2010;Sakata et al, 2005;Yatsunenko et al, 2012). The overrepresentation of bifidobacteria in this environment is less observed in formula-fed infants, who show a more diverse microbiota (Fallani et al, 2010;Penders et al, 2006).…”

Section: Bifidogenic Effect Of Hmomentioning

confidence: 96%

Consumption of human milk glycoconjugates by infant-associated bifidobacteria: mechanisms and implications

2013

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Human milk is a rich source of nutrients and energy, shaped by mammalian evolution to provide all the nutritive requirements of the newborn. In addition, several molecules in breast milk act as bioactive agents, playing an important role in infant protection and guiding a proper development. While major breast milk nutrients such as lactose, lipids and proteins are readily digested and consumed by the infant, other molecules, such as human milk oligosaccharides and glycosylated proteins and lipids, can escape intestinal digestion and transit through the gastrointestinal tract. In this environment, these molecules guide the composition of the developing infant intestinal microbiota by preventing the colonization of enteric pathogens and providing carbon and nitrogen sources for other colonic commensals. Only a few bacteria, in particular Bifidobacterium species, can gain access to the energetic content of milk as it is displayed in the colon, probably contributing to their predominance in the intestinal microbiota in the first year of life. Bifidobacteria deploy exquisite molecular mechanisms to utilize human milk oligosaccharides, and recent evidence indicates that their activities also target other human milk glycoconjugates. Here, we review advances in our understanding of how these microbes have been shaped by breast milk components and the strategies associated with their consumption of milk glycoconjugates.

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Section: Bifidogenic Effect Of Hmomentioning

confidence: 96%

Consumption of human milk glycoconjugates by infant-associated bifidobacteria: mechanisms and implications

2013

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“…1¡18 mo BF (7), FF (7) FISH, DGGE Similarity within and between the feeding groups was higher 10 weeks postweaning 55 (UK)…”

Section: (Finland)mentioning

confidence: 99%

“…24,45 Additional findings demonstrate that pre-weaning feeding mode remains associated with the post-weaning microbial communities. 18,33,55 For example, continued breastfeeding during the introduction of solid foods seems to support consistent levels of Lactobacillus spp. and Bifidobacterium spp.…”

Section: Weaning Complementary Feeding and Introduction Of Solidsmentioning

confidence: 99%

The role of early life nutrition in the establishment of gastrointestinal microbial composition and function

2017

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The development of the human infant intestinal microbiota is a sequential process that begins in utero and continues during the first 2 to 3 years of life. Microbial composition and diversity are shaped by host genetics and multiple environmental factors, of which diet is a principal contributor. An understanding of this process is of clinical importance as the microbiota acquired in early life influence gastrointestinal, immune and neural development, and reduced microbial diversity or dysbiosis during infancy is associated with disorders in infancy and later childhood. The goal of this article was to review the published literature that used culture-independent methods to describe the development of the gastrointestinal microbiota in breast-and formula-fed human infants as well as the impact of prebiotic and probiotic addition to infant formula, and the addition of solid foods.

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“…We investigated the development of faecal Bifidobacterium populations in relation to diet and age as part of a longitudinal study of the infant faecal microbiota (Roger & McCartney, 2010). The aim of this work was to monitor the infant faecal microbiota using a polyphasic approach that allowed examination of Bifidobacterium populations and detection of common or divergent colonization patterns in infants throughout the first 18 months of life.…”

Section: Introductionmentioning

confidence: 99%

Examination of faecal Bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life

et al. 2010

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Bifidobacteria in the infant faecal microbiota have been the focus of much interest, especially during the exclusive milk-feeding period and in relation to the fortification of infant formulae to better mimic breast milk. However, longitudinal studies examining the diversity and dynamics of the Bifidobacterium population of infants are lacking, particularly in relation to the effects of weaning. Using a polyphasic strategy, the Bifidobacterium populations of breast-and formula-fed infants were examined during the first 18 months of life. Bifidobacterium-specific denaturing gradient gel electrophoresis demonstrated that breast-fed infants harboured greater diversity than formula-fed infants and the diversity of the infants' Bifidobacterium populations increased with weaning. Twenty-seven distinctive banding profiles were observed from~1100 infant isolates using ribosomal intergenic spacer analysis, 14 biotypes of which were confirmed to be members of the genus Bifidobacterium. Two profiles (H, Bifidobacterium longum subsp. infantis; and I, Bifidobacterium bifidum) were common culturable biotypes, seen in 9/10 infants, while profile E (Bifidobacterium breve) was common among breast-fed infants. Overall, inter-and intra-individual differences were observed in the Bifidobacterium populations of infants between 1 and 18 months of age, although weaning was associated with increased diversity of the infant Bifidobacterium populations. Breast-fed infants generally harboured a more complex Bifidobacterium microbiota than formula-fed infants.

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Longitudinal investigation of the faecal microbiota of healthy full-term infants using fluorescence in situ hybridization and denaturing gradient gel electrophoresis

Cited by 96 publications

References 36 publications

Consumption of human milk glycoconjugates by infant-associated bifidobacteria: mechanisms and implications

Consumption of human milk glycoconjugates by infant-associated bifidobacteria: mechanisms and implications

The role of early life nutrition in the establishment of gastrointestinal microbial composition and function

Examination of faecal Bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life

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