Longitudinal histomechanical heterogeneity of the internal thoracic artery

Kostelnik, Colton; Crouse, Kiersten J; Carver, Wayne; Eberth, John F.

doi:10.1016/j.jmbbm.2021.104314

Cited by 4 publications

(7 citation statements)

References 34 publications

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“…20,21,38,39 Yet each of these methods, although largely effective at the removal of cellular material, can negatively impact the ECM microarchitecture limiting the tissue's inherent biophysical qualities. Starting with a novel but well-characterized match for the coronary vasculature 27 we chose the porcine ITA as a decellularization starting point and assessed the effects of varying DNase and anionic (SDS and SDC) detergents in a time-dependent manner. Our immersion-based chemical decellularization procedure retained tissue in an unloaded configuration to help minimize loading effects on the existing microarchitecture.…”

Section: Discussionmentioning

confidence: 99%

“…The ITA composition has been characterized as an elastic and musculoelastic artery, depending on the relative distance from the heart. 27,40,41 Heterogeneous distribution of elastin and collagen fibers influences the vascular wall mechanics and highlights the importance of donor tissue selectivity. 9,27 Histomechanical similarities exist between the ITA and the left anterior descending artery (LAD), with both arteries exhibiting similar compliance and a higher amount of elastin than other common autograft tissue sources.…”

Section: Discussionmentioning

confidence: 99%

“…27,40,41 Heterogeneous distribution of elastin and collagen fibers influences the vascular wall mechanics and highlights the importance of donor tissue selectivity. 9,27 Histomechanical similarities exist between the ITA and the left anterior descending artery (LAD), with both arteries exhibiting similar compliance and a higher amount of elastin than other common autograft tissue sources. 26 This histomechanical graft-target matching plays a vital role in long-term patency.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanical properties of the fresh, control, and decellularized ITAs were found through biaxial inflation-extension testing using a Bose Bio-Dynamic mechanical testing device following protocols described in. 27 The tissue sections were trimmed to comply with the testing device's 10 mm maximum displacement range, mounted onto two luer-fittings, and then fixed in place with 3-0 braided sutures. Then tissues were submerged in a testing bath and perfused with 1% PBS and sodium nitroprusside (10 À5 M) to elicit a fully passivated state.…”

Section: Biaxial Mechanical Testingmentioning

confidence: 99%

“…[23][24][25] These materials play a vital role in the mechanical properties of blood vessels which are deterministic in graft procedure outcomes. 26 Likewise, the analysis of comprehensive biaxial properties (i.e., inflation-extension) from a consistent material and anatomical location is fundamental to the efficacy of this biomaterial 27 and deviations in host-graft properties lead to atherosclerotic and inflammatory promoting perturbed flow profiles, shear stresses, and stress concentrations at the anastomosis site. 28 Many tissue-engineered decellularization strategies that fail to preserve the biochemical and biomechanical properties of native tissues possess poor cell repopulation and differentiation rates.…”

mentioning

confidence: 99%

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Small‐diameter artery decellularization: Effects of anionic detergent concentration and treatment duration on porcine internal thoracic arteries

et al. 2021

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Engineered replacement materials have tremendous potential for vascular applications where over 400,000 damaged and diseased blood vessels are replaced annually in the United States alone. Unlike large diameter blood vessels, which are effectively replaced by synthetic materials, prosthetic small-diameter vessels are prone to early failure, restenosis, and reintervention surgery. We investigated the differential response of varying 0%-6% sodium dodecyl sulfate and sodium deoxycholate anionic detergent concentrations after 24 and 72 h in the presence of DNase using biochemical, histological, and biaxial mechanical analyses to optimize the decellularization process for xenogeneic vascular tissue sources, specifically the porcine internal thoracic artery (ITA). Detergent concentrations greater than 1% were successful at removing cytoplasmic and cell surface proteins but not DNA content after 24 h. A progressive increase in porosity and decrease in glycosaminoglycan (GAG) content was observed with detergent concentration. Augmented porosity was likely due to the removal of both cells and GAGs and could influence recellularization strategies. The treatment duration on the other hand, significantly improved decellularization by reducing DNA content to trace amounts after 72 h. Prolonged treatment times reduced laminin content and influenced the vessel's mechanical behavior in terms of altered circumferential stress and stretch while further increasing porosity. Collectively, DNase with 1% detergent for 72 h provided an effective and efficient decellularization strategy to be employed in the preparation of porcine ITAs as bypass graft scaffolding materials with minor biomechanical and histological penalties.

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