Longitudinal evidence for immunosenescence and inflammaging in free-living great tits

Těšický, Martin; Krajzingrová, Tereza; Świderská, Zuzana; Syslová, Kamila; Bílková, Barbora; Eliáš, Jiří; Velová, Hana; Svobodová, Jana; Bauerová, Petra; Albrecht, Tomáš; Vinkler, Michal

doi:10.1016/j.exger.2021.111527

Cited by 5 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extent to which markers of immune aging displayed in mammals are shared in other vertebrates is to-date poorly understood 9 , with some evidence of immune senescence occurring in birds 10 .…”

Section: Introductionmentioning

confidence: 99%

Spontaneous onset of cellular markers of inflammation and genome instability during aging in the immune niche of the naturally short-lived turquoise killifish (Nothobranchius furzeri)

Morabito

Dönertaş

Seidel

et al. 2023

Preprint

View full text Add to dashboard Cite

Turquoise killifish (Nothobranchius furzeri) are naturally short-lived vertebrates, with a lifespan in captivity ranging from 4 to 8 months. Aging in turquoise killifish recapitulates several aspects of human aging, including protein aggregation, telomere shortening, neurodegeneration, cellular senescence, increased cancer incidence, age-dependent gut dysbiosis, decline in antibody diversity, and more. The mechanistic causes or systemic aging in killifish are still poorly understood. Here we ask whether killifish undergo significant age-dependent changes in the main hematopoietic organ, which together could contribute to systemic aging. We employed single-cell RNA sequencing, proteomics and cytometry, and provide an R Shiny app (KIAMO) to visualize and compare omics changes occurring during killifish aging. We find that old killifish display increased inflammatory markers both in plasma and in the main hematopoietic organ (kidney marrow). Immune cells from adult killifish display increased markers of proliferation and replication-independent DNA repair in progenitor-like cell clusters, while progenitors from old killifish display extensive markers of DNA double-strand breaks. In less than 10 weeks, from adulthood to geriatric age, killifish recapitulate several markers of aging of the immune niche, which could be functionally linked with its extensive systemic aging and serve as a target for anti-aging interventions.

show abstract

Section: Introductionmentioning

confidence: 99%

Spontaneous onset of cellular markers of inflammation and genome instability during aging in the immune niche of the naturally short-lived turquoise killifish (Nothobranchius furzeri)

Morabito

Dönertaş

Seidel

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The sampling was performed in a free-living great tit population breeding in artificial nest boxes in a deciduous forest at the edge of Prague, Czech Republic, EU (50°08’12.4”N, 14°27’57.2”E; see Těšický et al . 2021, 2022 for more details about study site) during their breeding period on April and May 2018.…”

Section: Methodsmentioning

confidence: 99%

“…The sampling was performed in a free-living great tit population breeding in artificial nest boxes in a deciduous forest at the edge of Prague, Czech Republic, EU (50˚08'12.4"N, 14˚27'57.2"E; see Těšický et al 2021Těšický et al , 2022 for more details about study site) during their breeding period on April and May 2018. In total, we collected 52 eggs to sample microbiome of egg content (E0-egg), 118 eggs to sample E13 embryonic intestine (unmanipulated, E13-nat, as well as Enterococcus-manipulated eggs, E13-Ent, see below), and 34 female faecal samples (see Table S2, SI1 for number of samples in different categories).…”

Section: Sampling Design and Sample Collectionmentioning

confidence: 99%

Nearly (?) sterile avian egg in a passerine bird

Těšický

Schmiedová

Krajzingrová

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

During early ontogeny, microbiome affects development of the gastrointestinal tract, immunity and survival in vertebrates. Bird egg has been suggested to be either (1) initially sterile (Sterile egg hypothesis) and (2) colonized through horizontal trans-shell migration after egg laying, or (3) initially seeded with bacteria through vertical transfer from mother oviduct. Little empirical data illuminate so far the contribution of these mechanisms to gut microbiota formation in avian embryos. We evaluated microbiome of the egg content (day 0; E0-egg), embryonic gut at day 13 (E13) and female faeces in a free-living passerine, the great tit (Parus major), using a methodologically advanced procedure combining 16S rRNA gene sequencing and microbe-specific qPCR assays. Our metabarcoding revealed that avian egg is (nearly) sterile, barely distinguishable in microbial composition from negative controls. Of the three potentially pathogenic bacteria targeted by qPCR, only Dietzia was found in E0-egg (yet also in controls), E13 gut and female samples, which might indicate its possible vertical transfer. Unlike in poultry, we have shown in passerines that major bacterial colonisation of the gut does not occur before chick hatching. We stress that protocols carefully checking for environmental contamination are critically important in studies using samples with low bacterial biomass.

show abstract

“…The sampling was conducted in a free-living great tit population breeding in artificial nest boxes in a deciduous forest at the edge of Prague, Czech Republic, EU (50˚08′12.4″N, 14˚27′57.2″E; see Těšický et al 2021 , 2022 for more details on the study site) during their breeding period in April and May 2018. In total, we collected 52 eggs to sample microbiome of the egg content (E0-egg), 118 eggs to sample E13 embryonic gut (unmanipulated, E13-nat, as well as Enterococcus -manipulated eggs, E13-Ent, see below), and 34 maternal female faecal samples (see Table S2 in Supporting Information 1 ( SI1 ) for number of samples in different categories).…”

Section: Methodsmentioning

confidence: 99%

Nearly (?) sterile avian egg in a passerine bird

Těšický,

Schmiedová,

Krajzingrová

et al. 2023

FEMS Microbiology Ecology

Self Cite

View full text Add to dashboard Cite

During early ontogeny, microbiome affects development of the gastrointestinal tract, immunity and survival in vertebrates. Bird eggs are thought to be (1) initially sterile (sterile egg hypothesis) and (2) colonised after oviposition through horizontal trans-shell migration, or (3) initially seeded with bacteria by vertical transfer from mother oviduct. To date, however, little empirical data illuminate the contribution of these mechanisms to gut microbiota formation in avian embryos. We investigated microbiome of the egg content (day 0; E0-egg), embryonic gut at day 13 (E13) and female faeces in a free-living passerine, the great tit (Parus major), using a methodologically advanced procedure combining 16S rRNA gene sequencing and microbe-specific qPCR assays. Our metabarcoding revealed that the avian egg is (nearly) sterile, yet obtaining a slightly richer microbiome during the embryonic development. Of the three potentially pathogenic bacteria targeted by qPCR, only Dietzia was found in E0-egg (yet also in controls), E13 gut and female samples, which might indicate possible vertical transfer. Unlike in poultry, we have shown in passerines that major bacterial colonisation of the gut does not occur before hatching. We emphasise that protocols that carefully check for environmental contamination are critical in studies with low-bacterial biomass samples.

show abstract

Longitudinal evidence for immunosenescence and inflammaging in free-living great tits

Cited by 5 publications

References 65 publications

Spontaneous onset of cellular markers of inflammation and genome instability during aging in the immune niche of the naturally short-lived turquoise killifish (Nothobranchius furzeri)

Spontaneous onset of cellular markers of inflammation and genome instability during aging in the immune niche of the naturally short-lived turquoise killifish (Nothobranchius furzeri)

Nearly (?) sterile avian egg in a passerine bird

Nearly (?) sterile avian egg in a passerine bird

Contact Info

Product

Resources

About