Longitudinal Dichelobacter nodosus status in 9 sheep flocks free from clinical footrot

Locher, Iwan; Greber, Deborah; Holdener, Kerstin; Lüchinger, Rita; Haerdi-Landerer, Christina; Schüpbach‐Regula, Gertraud; Frey, Joachim; Steiner, Adrian

doi:10.1016/j.smallrumres.2015.10.021

Cited by 22 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the current study, it detected symptomless carriers of virulent and benign D nodosus before clinical signs were manifest and proved thereby to be more sensitive than clinical scoring. The PCR results agreed with the clinical appearance of virulent and benign strains, including subclinical carriers, in agreement with previous observations [10,27,28]. Virulent D nodosus caused progressive underrunning footrot in flock 9 during the outbreak that followed sampling.…”

Section: Discussionsupporting

confidence: 91%

“…It can be concluded that clinical features are consistent with the PCR classification if benign is defined as non-progressive in the South German sheep population. This is in line with findings from Locher et al [27] in a previous study on clinically free sheep flocks in Switzerland, was described in Australia by Best et al [29] and also reported from Norwegian farms by Vatn et al [28]. Further, Best et al [29] proofed, that real-time PCR was significantly more sensitive in detection of aprV2 in clinically healthy sheep compared with culture/gelatinase test.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

The prevalence of Dichelobacter nodosus in clinically footrot-free sheep flocks: a comparative field study on elimination strategies

Kraft

Ströbel²,

Hilke³

et al. 2020

BMC Vet Res

View full text Add to dashboard Cite

Background: Ovine footrot caused by Dichelobacter nodosus (D nodosus) is an infectious disease affecting sheep worldwide. Switzerland plans a nationwide footrot eradication program, based on PCR-testing of interdigital swab samples. The aim of this study was to test for the presence of D nodosus in clinically footrot-free sheep flocks which had been subjected to different treatment strategies, to assess whether they were feasible for the eradication process, especially focussing on antimicrobial flock treatments. Clinical scoring and PCR-results were compared. Ten farms had used hoof bathing and hoof trimming without causing bleeding, ten had used individual treatments and flock vaccines to gain the free status and ten had become free through whole-flock systemic macrolide treatment. For every farm, three risk-based collected pool samples were analysed for the occurrence of virulent and benign D nodosus by PCR detection of aprV2/aprB2. Results: Six flocks from any treatment group tested positive for aprB2 in all pools. Clinical signs were absent at the time of sampling, but some flocks had experienced non-progressive interdigital inflammation previously. Two flocks tested aprV2-positive in the high-risk pool. One of them underwent a progressive footrot outbreak shortly after sampling. Individual retesting indicated, that virulent D nodosus most likely was reintroduced by a recently purchased ram. In the second flock, a ram was tested positive and treated before clinical signs occurred. Conclusions: All treatment strategies eliminated the causative agent and were found to be suitable for implementation in the PCR-based eradication process. PCR-testing proved to be more sensitive than visual scoring, as it also detected clinically healthy carriers. It will be of benefit as a diagnostic tool in elimination and surveillance programs.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

The prevalence of Dichelobacter nodosus in clinically footrot-free sheep flocks: a comparative field study on elimination strategies

Kraft

Ströbel²,

Hilke³

et al. 2020

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…Auch wenn es durch Therapie und/oder Keulung infizierter Tiere gelingen mag, die nach welcher Konvention auch immer als virulent klassifizierten Varianten von D. nodosus zu eliminieren, sollte dies im Sprachgebrauch vorerst nicht mit einer Eradikation von Moderhinke gleichgesetzt werden (58). Dies lehrt auch der jüngste flächenhafte Ausbruch von Moderhinke in New South Wales in Betrieben, die seit Jahrzehnten nach den australischen Regularien als frei von virulenter Moderhinke galten (11).…”

Section: Moderhinkefreiheitunclassified

Klaueninfektionen beim Schaf – Therapiemöglichkeiten in der tierärztlichen Praxis unter besonderer Berück sichtigung der Moderhinkebekämpfung

Ströbel¹,

Hilke²,

Spengler³

et al. 2018

Tierarztl Prax Ausg G

View full text Add to dashboard Cite

ZusammenfassungLahmheiten aufgrund von infektiösen Klauenerkrankungen bei Schafen besitzen nach wie vor eine hohe klinische Relevanz. Hinsichtlich der Therapie, insbesondere der Moderhinke, haben sich unter anderem durch die Neuzulassung von Medikamenten entscheidende Änderungen ergeben. Der Artikel gibt eine Übersicht über die Differenzialdiagnosen häufiger und seltener infektiöser Klauenerkrankungen beim Schaf und geht im Detail auf neue Erkenntnisse zu Ätiologie, Diagnostik, Prophylaxe, Behandlung und Kontrolle der Moderhinke ein. Trotz der Neuzulassung verschiedener antibiotischer Wirkstoffe zur Behand-lung der Moderhinke bleibt eine Umwidmung für Klauenbäder im Rahmen von integrierten Behandlungskonzepten in vielen Fällen unverzichtbar. Zur nachhaltigen Bekämpfung der Moderhinke sind die Umsetzung aktueller wissenschaftlicher Erkenntnisse in die Praxis und die Erarbeitung maßgeschneiderter Behandlungspläne für den jeweiligen Betrieb vonnöten. Der Artikel zeigt die verschiedenen Behandlungsoptionen sowie Wege zu Kontrolle und Sanierung auf.

show abstract

“…The samples analyzed and used for evaluation of the different pooling methods were collected from October 2013 until May 2016 from 2 published studies, one a proof-of-concept study for flock treatment 9 and the other an evaluation of the prevalence of D. nodosus in a longitudinal study. 14…”

Section: Selection Of Sheep and Status Definition Of Flocks And Sheepmentioning

confidence: 99%

Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus

et al. 2017

Self Cite

View full text Add to dashboard Cite

Virulent ovine foot rot is a contagious foot disease. Given the development and validation of a real-time PCR to detect Dichelobacter nodosus isolates that contain the virulence-associated protease genes aprV2 and aprB2, the diagnosis of foot rot has made considerable progress. We evaluated pooling methods to reduce the number of samples during a foot rot control program. Samples of individual feet were compared to a 4-feet sample of the same sheep. All further analyses based on 4-feet samples (pools-of-5 and pools-of-10 4-feet samples) were compared to samples of individual sheep, and a risk-based herd sampling was evaluated and compared to the whole flock. The sensitivity and specificity of the 4-feet samples for detection of aprV2-positive strains was 93.8% (CI: 87.6-97.5%) and 98.3% (CI: 96.5-99.3%), respectively. The sensitivity and specificity of the pools-of-10 was 86.7% (CI: 78.4-92.7%) and 100.0% (CI: 97.4-100%), respectively. Pools-of-5 were not significantly more sensitive than pools-of-10. The pooling of 4 individual foot samples into one 4-feet sample is an adequate method to reduce the number of samples of individual sheep. The sensitivity of pools-of-5 and pools-of-10 is too imprecise for a control program. Risk-based sampling allowed for a substantial reduction of samples to be tested, had a sensitivity of 95.8% (CI: 78.9-99.9%) and specificity of 100.0% (CI: 88.1-100.0%) when determining the foot rot flock status, and represents an adequate methodology to predict within-flock freedom from infection.

show abstract

Longitudinal Dichelobacter nodosus status in 9 sheep flocks free from clinical footrot

Cited by 22 publications

References 32 publications

The prevalence of Dichelobacter nodosus in clinically footrot-free sheep flocks: a comparative field study on elimination strategies

The prevalence of Dichelobacter nodosus in clinically footrot-free sheep flocks: a comparative field study on elimination strategies

Klaueninfektionen beim Schaf – Therapiemöglichkeiten in der tierärztlichen Praxis unter besonderer Berück sichtigung der Moderhinkebekämpfung

Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus

Contact Info

Product

Resources

About