Longitudinal detection of retinal alterations by visible and near-infrared optical coherence tomography in a dexamethasone-induced ocular hypertension mouse model

Song, Weiye; Fu, Sipei; Song, Shangshang; Zhang, Sui; Zhang, Lei; Ness, Steven; Desai, Manishi; Yi, Ji

doi:10.1117/1.nph.6.4.041103

Cited by 9 publications

(9 citation statements)

References 54 publications

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“…Compared to standard OCT operating in the near-infrared band, visible-light OCT (vis-OCT) (24, 25) produces higher axial resolution (26,27) and higher spectral contrast between oxy-and deoxy-hemoglobin (8). Using vis-OCT, oximetry on major vessels in rodents and humans has been successfully demonstrated (24,(28)(29)(30)(31) and used to monitor how progressive hypoxic challenge (32) or intraocular pressure (IOP) elevation (33,34) affects retinal oxygen metabolism. Advances in automated detection of posterior vascular boundaries (29), acquisition of multiple circumpapillary scans (31), and quantitative quality controls (35) have been made to improve retinal oximetry on major vessels.…”

mentioning

confidence: 99%

Retinal capillary oximetry with visible light optical coherence tomography

Hormel

Wei

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Assessing oxygen saturation (sO2) remains challenging but is nonetheless necessary for understanding retinal metabolism. We and others previously achieved oximetry on major retinal vessels and measured the total retinal oxygen metabolic rate in rats using visible-light optical coherence tomography. Here we extend oximetry measurements to capillaries and investigate all three retinal vascular plexuses by amplifying and extracting the spectroscopic signal from each capillary segment under the guidance of optical coherence tomography (OCT) angiography. Using this approach, we measured capillary sO2in the retinal circulation in rats, demonstrated reproducibility of the results, validated the measurements in superficial capillaries with known perfusion pathways, and determined sO2responses to hypoxia and hyperoxia in the different retinal capillary beds. OCT capillary oximetry has the potential to provide new insights into the retinal circulation in the normal eye as well as in retinal vascular diseases.

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mentioning

confidence: 99%

Retinal capillary oximetry with visible light optical coherence tomography

Hormel

Wei

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…To measure the physical dimension of the artificial retina, OCT imaging was performed on the eye model. The theoretical resolutions in axial and transverse direction for our OCT setup were ~5.6 µm and ~10.2 µm [30], respectively, providing sufficient measurement accuracy. Figure 3 The thickness of the artificial retina was measured to be ~0.8mm.…”

Section: The Design Of the Human Eye Modelmentioning

confidence: 86%

“…An human eye OCT system described from our previous publications [30,31] were used to characterize the physical dimension of the retina phantom and provide OCT angiography (OCTA) as a simulation image for oSLO in human FA. The system used 800-880 nm laser as light source.…”

Section: Oct Imagingmentioning

confidence: 99%

Volumetric fluorescence imaging in a human eye model by oblique scanning laser ophthalmoscope (oSLO): a feasibility study

Shao

Song

Jiang

2019

Preprint

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Fluorescence retinal imaging, such as fluorescein angiography, indocyanine green angiography, and autofluorescence imaging, are valuable tools in ophthalmology and vision science. However, these clinical imaging modalities provide en face view of the retina, with limited capability to discriminate retinal layers over a large field-of-view (FOV). We recently developed a novel retinal imaging method, oblique scanning laser ophthalmoscopy (oSLO), to provide volumetric retinal fluorescence imaging without any depth sectioning. OSLO breaks the coaxial alignment of the excitation and detection, to produce a cross-sectional view on retina using the natural ocular optics. In this paper, we demonstrated oSLO in a realistic human eye model and showed the feasibility for future in vivo human retinal imaging. A new optical design was implemented to significantly simplify our previous oSLO systems. We overcame the limitation by the small numerical aperture (NA) of the human eye, by integrating a pair of cylindrical lens in the remote focusing system. We experimentally showed that the current setup can achieve a FOV of ~3×6×0.8 mm 3 , and the transverse and axial resolutions of 7 and 41 µm, respectively. The capability of volumetric fluorescence imaging over a large FOV in the human retina could lead to new clinical imaging paradigms for retinal diseases.

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“…pVN) was defined from reflectance spectroscopy, consisting of the intensity ratio between VIS-OCT and NIR-OCT images. We identified changes in RNFL of ocular hypertensive mice using pVN 26 . The goal of the present study was to compare our two-channel VIS-OCT metrics to standard OCT outcomes among normal, glaucoma suspect and glaucoma patients in a cross-sectional study.…”

Section: Introductionmentioning

confidence: 99%

Visible light optical coherence tomography of peripapillary retinal nerve fiber layer reflectivity in glaucoma

Song

Zhang

Kim

et al. 2021

Preprint

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Purpose: To evaluate the clinical utility of visible light optical coherence tomography (VIS-OCT) and to test whether VIS-OCT reflectivity and spectroscopy of peripapillary retinal nerve fiber layer (pRNFL) are correlated with severity of glaucoma, compared with standard-of-care OCT thickness measurements. Design: Cross-sectional study. Method: Fifty-four eyes from three groups of subjects (normal, glaucoma suspect, and glaucoma) were scanned by a custom-designed dual-channel device that simultaneously acquired visible (VIS-OCT) and near-infrared OCT (NIR-OCT) images. A 5x5 mm2 scan was taken of the peripapillary nerve fiber layer (pRNFL). The pRNFL reflectivity was calculated for both channels and the spectroscopic marker was quantified by pVN, defined by the ratio of VIS-OCT to NIR-OCT pRNFL reflectivity. The results were compared with ophthalmic exams and clinical Zeiss OCT measurements. Mixed linear model was used to evaluate the association of imaging markers with glaucoma severity. Results: VIS-OCT pRNFL reflectivity significantly, sequentially declined from normal to suspect to glaucoma (3.42 ± 0.35, 2.58 ± 0.37, 2.16 ± 0.39, mean ± SD), as did NIR-OCT pRNFL reflectivity (2.46 ± 0.24, 2.27 ± 0.24, 1.96 ± 0.25). The pVN also had the same decreasing trend among three groups (1.39 ± 0.08, 1.14 ± 0.08, 1.08 ± 0.10). Normal and suspect eyes were significantly different in VIS-OCT pRNFL reflectivity (p=0.002), pVN (p<0.001) but not in NIR-OCT pRNFL reflectivity (p=0.14), circumpapillary RNFL (cpRNFL) thickness (p=0.17), or macular ganglion cell layer and inner plexiform layer (GCL+IPL) thickness (p=0.07) in the mixed linear model. Conclusion: VIS-OCT pRNFL reflectivity and pVN was more sensitive in separating suspect eyes from normal ones than clinical OCT thickness measurements. VIS-OCT pRNFL reflectivity and pVN could be a useful metric in early detection of glaucoma upon further longitudinal validation.

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Longitudinal detection of retinal alterations by visible and near-infrared optical coherence tomography in a dexamethasone-induced ocular hypertension mouse model

Cited by 9 publications

References 54 publications

Retinal capillary oximetry with visible light optical coherence tomography

Retinal capillary oximetry with visible light optical coherence tomography

Volumetric fluorescence imaging in a human eye model by oblique scanning laser ophthalmoscope (oSLO): a feasibility study

Visible light optical coherence tomography of peripapillary retinal nerve fiber layer reflectivity in glaucoma

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