Longitudinal Circulating Tumor DNA Analysis in Blood and Saliva for Prediction of Response to Osimertinib and Disease Progression in EGFR-Mutant Lung Adenocarcinoma

Kim, Chul; Xi, Liqiang; Cultraro, Constance M.; Fang, Wei; Jones, Gregory R.; Cheng, Jordan; Shafiei, Ahmad; Pham, Trinh Hoc-Tran; Roper, Nitin; Akoth, Elizabeth; Ghafoor, Azam; Misra, Vikram; Monkash, Nina; Strom, Charles M.; Tu, Manghui; Liao, Wei; Morris, Clive; Steinberg, Seth M.; Bagheri, Hadi; Wong, David T.; Guha, Udayan

doi:10.3390/cancers13133342

Cited by 15 publications

(6 citation statements)

References 37 publications

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“…Tumor DNA can reach the saliva either through direct shedding from a mucosal tumor or filtering from plasma within salivary glands [ 31 ]. This notion that saliva is a filtered version of blood plasma has been supported by observations that liquid biopsy genotyping of lung cancer is concordant between plasma and salivary DNA [ 32 ].…”

Section: Discussionmentioning

confidence: 94%

Isolation of salivary cell-free DNA for cancer detection

et al. 2023

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Saliva is an emerging source of disease biomarkers, particularly for cancers of the head and neck. Although analysis of cell-free DNA (cfDNA) in saliva holds promise as a liquid biopsy for cancer detection, currently there are no standardized methodologies for the collection and isolation of saliva for the purposes of studying DNA. Here, we evaluated various saliva collection receptacles and DNA purification techniques, comparing DNA quantity, fragment size, source, and stability. Then, using our optimized techniques, we tested the ability to detect human papillomavirus (HPV) DNA– a bona fide cancer biomarker in a subset of head and neck cancers– from patient saliva samples. For saliva collection, we found that the Oragene OG-600 receptacle yielded the highest concentration of total salivary DNA as well as short fragments <300 bp corresponding to mononucleosomal cell-free DNA. Moreover, these short fragments were stabilized beyond 48 hours after collection in contrast to other saliva collection receptacles. For DNA purification from saliva, the QIAamp Circulating Nucleic Acid kit yielded the highest concentration of mononucleosome-sized DNA fragments. Freeze-thaw of saliva samples did not affect DNA yield or fragment size distribution. Salivary DNA isolated from the OG-600 receptacle was found to be composed of both single and double-stranded DNA, including mitochondrial and microbial sources. While levels of nuclear DNA were consistent over time, levels of mitochondrial and microbial DNA were more variable and increased 48 hours after collection. Finally, we found that HPV DNA was stable in OG-600 receptacles, was reliably detected within the saliva of patients with HPV-positive head and neck cancer, and was abundant among mononucleosome-sized cell-free DNA fragments. Our studies have defined optimal techniques for isolating DNA from saliva that will contribute to future applications in liquid biopsy-based cancer detection.

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Section: Discussionmentioning

confidence: 94%

Isolation of salivary cell-free DNA for cancer detection

et al. 2023

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“…This approach is not only suitable for primary tumor tissue but is routinely used in the transbronchial needle aspiration of lymph node [ 87 ], plasma [ 88 ], and pleural effusions [ 89 ] and could also be used from bronchoalveolar lavage fluid [ 90 ] and is also suitable for cerebrospinal fluid [ 91 ]. Besides, there are promising data for the screening of residual disease of EGFR mutant NSCLC in saliva [ 92 ] and urine [ 93 ].…”

Section: From Multiple Companion Biomarkers To Ngs?mentioning

confidence: 99%

Molecular Profiling in Non-Squamous Non-Small Cell Lung Carcinoma: Towards a Switch to Next-Generation Sequencing Reflex Testing

Pujol

Heeke

Bontoux

et al. 2022

JPM

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Molecular diagnosis of lung cancer is a constantly evolving field thanks to major advances in precision oncology. The wide range of actionable molecular alterations in non-squamous non-small cell lung carcinoma (NS-NSCLC) and the multiplicity of mechanisms of resistance to treatment resulted in the need for repeated testing to establish an accurate molecular diagnosis, as well as to track disease evolution over time. While assessing the increasing complexity of the molecular composition of tumors at baseline, as well as over time, has become increasingly challenging, the emergence and implementation of next-generation sequencing (NGS) testing has extensively facilitated molecular profiling in NS-NSCLC. In this review, we discuss recent developments in the molecular profiling of NS-NSCLC and how NGS addresses current needs, as well as how it can be implemented to address future challenges in the management of NS-NSCLC.

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“…Lung cancer is the most commonly diagnosed malignancy worldwide and lung adenocarcinoma (LUAD) represents the most common histological type of lung cancer (69)(70)(71)(72)(73). A late diagnosis of LUAD contributes to high metastasis and mortality rates, emphasizing the urgency for better identification of sensitive biomarkers during lung cancer progression (69,(74)(75)(76).…”

Section: The Tumor-promoting Role Of Draic In Cancers Lung Cancermentioning

confidence: 99%

The emerging potentials of lncRNA DRAIC in human cancers

2022

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Long non-coding RNA (lncRNA) is a subtype of noncoding RNA that has more than 200 nucleotides. Numerous studies have confirmed that lncRNA is relevant during multiple biological processes through the regulation of various genes, thus affecting disease progression. The lncRNA DRAIC, a newly discovered lncRNA, has been found to be abnormally expressed in a variety of diseases, particularly cancer. Indeed, the dysregulation of DRAIC expression is closely related to clinicopathological features. It was also reported that DRAIC is key to biological functions such as cell proliferation, autophagy, migration, and invasion. Furthermore, DRAIC is of great clinical significance in human disease. In this review, we discuss the expression signature, clinical characteristics, biological functions, relevant mechanisms, and potential clinical applications of DRAIC in several human diseases.

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Longitudinal Circulating Tumor DNA Analysis in Blood and Saliva for Prediction of Response to Osimertinib and Disease Progression in EGFR-Mutant Lung Adenocarcinoma

Cited by 15 publications

References 37 publications

Isolation of salivary cell-free DNA for cancer detection

Isolation of salivary cell-free DNA for cancer detection

Molecular Profiling in Non-Squamous Non-Small Cell Lung Carcinoma: Towards a Switch to Next-Generation Sequencing Reflex Testing

The emerging potentials of lncRNA DRAIC in human cancers

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