2015
DOI: 10.1186/s13073-015-0239-y
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Longitudinal analysis of the peripheral B cell repertoire reveals unique effects of immunization with a new influenza virus strain

Abstract: BackgroundDespite the potential to produce antibodies that can neutralize different virus (heterotypic neutralization), there is no knowledge of why vaccination against influenza induces protection predominantly against the utilized viral strains (homotypic response). Identification of structural patterns of the B cell repertoire associated to heterotypic neutralization may contribute to identify relevant epitopes for a universal vaccine against influenza.MethodsBlood samples were collected from volunteers imm… Show more

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Cited by 37 publications
(39 citation statements)
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“…In addition, the repertoires that we report here are in good agreement with those reported in previous studies for both humans and RMs. 34, 40, 52, 57, 58, 59, 60, 61 Taken together, these findings provide confidence that the BCR repertoires we report here are representative of actual average circulating repertoires in both species, although the collection of more repertoire data will be needed to confirm this.…”
Section: Discussionsupporting
confidence: 66%
“…In addition, the repertoires that we report here are in good agreement with those reported in previous studies for both humans and RMs. 34, 40, 52, 57, 58, 59, 60, 61 Taken together, these findings provide confidence that the BCR repertoires we report here are representative of actual average circulating repertoires in both species, although the collection of more repertoire data will be needed to confirm this.…”
Section: Discussionsupporting
confidence: 66%
“…The latter, can be visualized either through heatmaps, histograms or pie charts. Furthermore, if a 3ʹ primer was set to cover the C region during the library construction, it is possible to perform an analysis to identify the most abundant isotype; this is especially relevant when conducting protocols for "before-and-after" vaccination and their respective repertoire analysis (3,9,12,14,(40)(41)(42). The statistical quantification and comparison of clonotype diversity is primarily calculated using the generalized diversity index -a general mathematical formulation commonly used in ecology to assess diversity, developed by Hill in 1973(43).…”
Section: Diversity Quantification and Analysis Of The Sequenced Samplementioning
confidence: 99%
“…When the immune system encounters an antigen, memory cells may be activated to produce already existent antibodies or naive B cells may hypermutate the variable regions of the antibody thus forming a B cell lineage with new plasma cells producing new antibodies (3). Analyzing how the antibody repertoire evolves throughout time provides an insight into how pathogens, vaccines, and even self-epitopes shape our humoral response (3,9,12,14,(40)(41)(42)52,53). Although the evolution of repertoires can be studied at one single time point with phylogenetic trees that portray clonal dynamics, multiple time points help to generate more accurate and robust ontogenic analysis and these can be portrayed as stream graphs, longitudinal phylogenetic "birthday" trees, stack-plots or clonotype tracking heatmaps.…”
Section: Evolution Of Repertoire/ Clonal Dynamicsmentioning
confidence: 99%
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“…Subsequently, the region will be cleaved and replaced by the gBlock [109]. This method has allowed the design and generation of antibody libraries [117,118].…”
Section: De Novo Synthesis Of Antibody Genesmentioning
confidence: 99%