Longitudinal analysis of taurine induced effects on the tear proteome of contact lens wearers and dry eye patients using a RP-RP-Capillary-HPLC–MALDI TOF/TOF MS approach

Funke, Sebastian; Azimi, Diana; Wolters, Dominik; Grus, Franz H.; Pfeiffer, Norbert

doi:10.1016/j.jprot.2012.03.018

Cited by 32 publications

(19 citation statements)

References 69 publications

(76 reference statements)

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“…transcranial ultrasound of the substantia nigra and measurements of nerve conduction, tests of cognitive function, olfactory testing and collection of CSF and blood samples. These will be evaluated by using Bottom Up LC ESI MS [51,52,[89][90][91]. This analysis should thus give a first insight on the proteomic alterations in PD patients and could provide candidate proteins to be verified as biomarkers in further prospective studies (Fig.…”

Section: Discussionmentioning

confidence: 99%

Biomarker sources for Parkinson's disease: Time to shed tears?

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Biomarker sources for Parkinson's disease: Time to shed tears?

et al. 2015

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“…The LC-system consisted of a Rheos Allegro pump (Thermo Scientific, Rockford, USA) and a PAL HTC autosampler (CTC Analytics, Zwingen, Switzerland), as described elsewhere [33]. The system comprised of a 30 × 0.5 mm BioBasic C18 precolumn (Thermo Scientific) connected to a 150 × 0.5 mm BioBasic C18 column (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the human aqueous humour proteome: A comparison of the genders

et al. 2017

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Aqueous humour (AH) is an important biologic fluid that maintains normal intraocular pressure and contains proteins that regulate the homeostasis of ocular tissues. Any alterations in the protein compositions are correlated to the pathogenesis of various ocular disorders. In recent years, gender-based medicine has emerged as an important research focus considering the prevalence of certain diseases, which are higher in a particular sex. Nevertheless, the inter-gender variations in the AH proteome are unknown. Therefore, this study endeavoured to characterize the AH proteome to assess the differences between genders. Thirty AH samples of patients who underwent cataract surgery were categorized according to their gender. Label-free quantitative discovery mass spectrometry-based proteomics strategy was employed to characterize the AH proteome. A total of 147 proteins were identified with a false discovery rate of less than 1% and only the top 10 major AH proteins make up almost 90% of the total identified proteins. A large number of proteins identified were correlated to defence, immune and inflammatory mechanisms, and response to wounding. Four proteins were found to be differentially abundant between the genders, comprising SERPINF1, SERPINA3, SERPING1 and PTGDS. The findings emerging from our study provide the first insight into the gender-based proteome differences in the AH and also highlight the importance in considering potential sex-dependent changes in the proteome of ocular pathologies in future studies employing the AH.

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“…An important reason of DES is the wearing of contact lens, in fact, by using proteomics approaches, Funke et al. revealed distinct similarities between contact lens wearers and DES patients. Moreover, Nichols et al.…”

Section: Tear Protein Biomarkers In Ocular Diseasesmentioning

confidence: 99%

“…Results demonstrated that different laser platforms induce distinct biological responses in the cornea and ocular surface, which manifests as different tear proteins composition. An important reason of DES is the wearing of contact lens, in fact, by using proteomics approaches, Funke et al [34] revealed distinct similarities between contact lens wearers and DES patients. Moreover, Nichols et al [35] highlighted differential protein expression in contact lens wearers that occur in DES with respect to normal contact lens wearers.…”

Section: Keratoconjunctivitis Siccamentioning

confidence: 99%

Unraveling the molecular repertoire of tears as a source of biomarkers: Beyond ocular diseases

Pieragostino

D'Alessandro

Ioia

et al. 2015

Proteomics Clinical Apps

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Proteomics and metabolomics investigations of body fluids present several challenges for biomarker discovery of several diseases. The search for biomarkers is actually conducted in different body fluids, even if the ideal biomarker can be found in an easily accessible biological fluid, because, if validated, the biomarker could be sought in the healthy population. In this regard, tears could be considered an optimum material obtained by noninvasive procedures. In the past years, the scientific community has become more interested in the study of tears for the research of new biomarkers not only for ocular diseases. In this review, we provide a discussion on the current state of biomarkers research in tears and their relevance for clinical practice, and report the main results of clinical proteomics studies on systemic and eye diseases. We summarize the main methods for tear samples analyses and report recent advances in "omics" platforms for tears investigations. Moreover, we want to take stock of the emerging field of metabolomics and lipidomics as a new and integrated approach to study protein-metabolites interplay for biomarkers research, where tears represent a still unexplored and attractive field.

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Longitudinal analysis of taurine induced effects on the tear proteome of contact lens wearers and dry eye patients using a RP-RP-Capillary-HPLC–MALDI TOF/TOF MS approach

Cited by 32 publications

References 69 publications

Biomarker sources for Parkinson's disease: Time to shed tears?

Biomarker sources for Parkinson's disease: Time to shed tears?

Characterization of the human aqueous humour proteome: A comparison of the genders

Unraveling the molecular repertoire of tears as a source of biomarkers: Beyond ocular diseases

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