Longitudinal 16S rRNA Sequencing Reveals Relationships among Alterations of Gut Microbiota and Nonalcoholic Fatty Liver Disease Progression in Mice

Zhuge, Aoxiang; Li, Shengjie; Lou, Pengcheng; Wu, Wenrui; Wang, Kaiceng; Yuan, Yin; Xia, Jiafeng; Li, Bo; Li, Lanjuan

doi:10.1128/spectrum.00047-22

Cited by 36 publications

(27 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Jiang’s study also found that the relative abundance of Lactobacillus was increased in NAFLD patients compared to healthy subject [ 57 ]. Alistipes is considered to be a specific biomarker of oxidative damage to hepatic cells, fibrosis, and gut microbiota disturbances in NAFLD patients [ 58 ]. Eubacterium coprostanoligenes group and Ruminococcaceae UCG 002 belong to Ruminococcaceae, which was thought to promote hepatocyte fibrosis in NAFLD patients [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Interplay of Lymphocytes with the Intestinal Microbiota in Children with Nonalcoholic Fatty Liver Disease

Liang

Zunong

et al. 2022

Nutrients

View full text Add to dashboard Cite

Abnormally high lymphocyte counts are seen in persons with nonalcoholic fatty liver disease (NAFLD). Gut microbiota dysbiosis is a risk factor for NAFLD. We assessed the gut microbiota of 63 healthy children and 63 children with NAFLD using 16S rRNA gene and metagenomic sequencing to explore the relationships. Compared with healthy children (HC group), the Bacteroidetes, Verrucomicrobia, and Akkermansia were less abundant, while the Actinobacteria were more abundant in children with NAFLD (FLD group). To understand the effect of lymphocytes on the gut microbiota of children with NAFLD, we compared the microbiota of 41 children with NAFLD and high numbers of lymphocytes (FLD_HL group) and 22 children with NAFLD and low numbers of lymphocytes (FLD_LL group). The abundances of Bacteroidetes, Verrucobacterium, and Akkermansia increased and Actinobacteria decreased in the FLD_LL group compared to the FLD_HL group. Akkermansia was negatively correlated with lymphocyte count. NAFLD may disturb the gut microbiota in children through reducing the abundance of Akkermansia and increasing the abundance of proinflammatory bacteria, such as Escherichia-Shigella. Conclusions: High lymphocyte counts are associated with disturbances of gut microbiota and emergence of opportunistic pathogens in children with NAFLD.

show abstract

Section: Discussionmentioning

confidence: 99%

Interplay of Lymphocytes with the Intestinal Microbiota in Children with Nonalcoholic Fatty Liver Disease

Liang

Zunong

et al. 2022

Nutrients

View full text Add to dashboard Cite

show abstract

“…Studies have shown that reducing the abundance of Blautia may help to reduce obesity, reduce blood lipid levels and regulate blood glucose homeostasis ( Zhao et al, 2022 ). Dubosiella was positively correlated with liver injury, lipid metabolism, and fibrosis ( Zhuge et al, 2022 ). Other studies have shown that the intestinal disorder induced by diabetes can be reversed by increasing the abundance of Blautia and Dubosiella ( Pan et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous analysis shows that the relationship between biochemical indexes, metabolites, and IM are closely related, which is of great significance to the occurrence and treatment of T2DM. Dubosiella was positively correlated with liver injury and lipid metabolism ( Zhuge et al, 2022 ). Romboutsia and UCG-005 were negatively correlated with inflammation ( Li et al, 2021 ; Wang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Bear bile powder ameliorates type 2 diabetes via modulation of metabolic profiles, gut microbiota, and metabolites

et al. 2023

View full text Add to dashboard Cite

Introduction: Bear bile powder (BBP) is widely used in the clinic and has a hypoglycemic effect, but its mechanism is not clear.Methods: In this study, type 2 diabetes mellitus (T2DM) rats induced by a high-sugar and high-fat diet combined with streptozotocin were given BBP, and biochemical indexes, pathological sections, metabonomics, intestinal microbiota (IM) and short-chain fatty acids (SCFAs) were determined.Results: The results showed that BBP could reduce blood glucose, relieve inflammation, insulin resistance, and lipid metabolism disorder, and alleviate tissue damage of the liver, spleen, kidney, and pancreas in T2DM rats. It is worth noting that BBP can reverse the changes in blood and urine metabolites in T2DM rats, which are mainly related to tryptophan metabolism, pentose and glucuronate interconversions, starch and sucrose metabolism, and glycerophospholipid metabolism. In addition, BBP restored IM disorder in T2DM rats, decreased the abundance of Allobaculum, Blautia, Dubosiella, and Anaerostipes, enriched the abundance of Lactobacillus, Romboutsia, UCG-005, and norank_f__Eggerthellaceae, and increased the concentration of SCFAs in intestinal contents.Discussion: These findings suggest that BBP may improve T2DM by regulating multiple metabolic pathways, IM composition, and SCFAs levels.

show abstract

“…Demir et al found that the faecal fungi composition in fibrosis patients was characterized by a higher log-ratio of Mucor sp./Saccharomyces cerevisiae [ 11 ]. In the western diet-fed mice, gradually reducing abundances of Clostridia and Ruminococcaceae followed the occurrence of NAFLD [ 12 ]. Furthermore, the connection between gut flora and NAFLD was generally explored in recent years.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Hydroxyphenyl Propionic Acids on Lipid Metabolism and Gut Microbiota in Mice Fed a High-Fat Diet

et al. 2023

View full text Add to dashboard Cite

Gut microbiota imbalances lead to the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is primarily accompanied by hepatic steatosis. Hydroxyphenyl propionic acids (HPP) have shown great potential in inhibiting lipid accumulation but their protective effects concerning NAFLD and intestinal microbiota have remained unclear. In this paper, we investigated the efficacies of 3-HPP and 4-HPP on hepatic steatosis and gut flora in mice fed a high-fat diet (HFD). We found that 3-HPP and 4-HPP administration decreased body weight and liver index, ameliorated dyslipidemia, and alleviated hepatic steatosis. Furthermore, 3-HPP and 4-HPP enhanced the multiformity of gut microbiota; improved the relative abundance of GCA-900066575, unidentified_Lachnospiraceae, and Lachnospiraceae_UCG-006 at genus level; increased concentration of acetic acid, propionic acid and butanoic acid in faeces; and reduced systemic endotoxin levels in NAFLD mice. Moreover, 4-HPP upregulated the relative abundance of genera Rikenella and downregulated the relative abundance of Faecalibaculum. Furthermore, 3-HPP and 4-HPP regulated lipid metabolism and ameliorated gut dysbiosis in NAFLD mice and 4-HPP was more effective than 3-HPP.

show abstract

Longitudinal 16S rRNA Sequencing Reveals Relationships among Alterations of Gut Microbiota and Nonalcoholic Fatty Liver Disease Progression in Mice

Cited by 36 publications

References 64 publications

Interplay of Lymphocytes with the Intestinal Microbiota in Children with Nonalcoholic Fatty Liver Disease

Interplay of Lymphocytes with the Intestinal Microbiota in Children with Nonalcoholic Fatty Liver Disease

Bear bile powder ameliorates type 2 diabetes via modulation of metabolic profiles, gut microbiota, and metabolites

Protective Effects of Hydroxyphenyl Propionic Acids on Lipid Metabolism and Gut Microbiota in Mice Fed a High-Fat Diet

Contact Info

Product

Resources

About