Longevity-Associated Core Gut Microbiota Mining and Effect of Mediated Probiotic Combinations on Aging Mice: Case Study of a Long-Lived Population in Guangxi, China

Abstract

With an ageing population, healthy longevity is becoming an important scientific concern. The longevity phenomenon is closely related to the intestinal microflora and is highly complicated; it is challenging to identify and define the core gut microbiota associated with longevity. Therefore, in this study, 16S rRNA sequencing data were obtained from a total of 135 faecal samples collected as part of the latest sampling and pre-collection initiative in the Guangxi longevity area, and weighted gene co-expression network analysis (WGCNA) was used to find a mediumpurple3 network module significantly associated with the Guangxi longevity phenomenon. Five core genera, namely, Alistipes, Bacteroides, Blautia, Lachnospiraceae NK4A136 group, and Lactobacillus, were identified via network analysis and random forest (RF) in this module. Two potential probiotic strains, Lactobacillus fermentum and Bacteroides fragilis, were further isolated and screened from the above five core genera, and then combined and used as an intervention in naturally ageing mice. The results show a change in the key longevity gut microbiota in mice toward a healthy longevity state after the intervention. In addition, the results show that the probiotic combination effectively ameliorated anxiety and necrosis of hippocampal neuronal cells in senescent mice, improving their antioxidant capacity and reducing their inflammation levels. In conclusion, this longer-term study provides a new approach to the search for longevity hub microbiota. These results may also provide an important theoretical reference for the healthification of the intestinal microflora in the general population, and even the remodelling of the structure of the longevity-state intestinal microflora.

Structural characteristics of gut microbiota in longevity from Changshou town, Hubei, China

“…However, this contradicts previous reports of Bacteroides enrichment in long-lived elderly individuals. (Park et al 2015b ; Li et al 2023 ; Pang et al 2023 ). Bacteroides are crucial microorganisms in the human intestine.…”

Winds of change a tale of: asthma and microbiome

“…Regarding the gut microbiome, host genetics, and asthma, Li et al, described that, through a two-sample Mendelian randomization analysis, they predicted a positive correlation between the gut species Barnesiella and RuminococcaceaeUCG014 genera and the risk of asthma. Furthermore, they found that Akkermansia reduced the risk of adult-onset asthma ( Li et al, 2023 ). Perez-Garcia et al, through a study of microbiome- genome wide association (mbGWAS) and microbiome quantitative trait loci (mbQTL) analysis, reported the identification of polymorphisms in the APOBEC3B-APOBEC3c, TRIM24, and TPST2 genes that are associated with asthma comorbidities.…”

“…Several studies have shown that the use of antibiotics in early childhood increases the risk for developing asthma up to 2.3 times ( Kim et al, 2018 ; Lin et al, 2020 ; Li et al, 2023 ), these risks increase even more with the number of antibiotics courses prescribed. In infants (<1 year) antibiotic use is associated with a 24% higher incidence of asthma for every 10% increase in prescriptions ( Patrick et al, 2020 ).…”

“…In infants (<1 year) antibiotic use is associated with a 24% higher incidence of asthma for every 10% increase in prescriptions ( Patrick et al, 2020 ). Among the most prescribed are B-lactams such as amoxicillin (2-fold), followed by macrolides, second-generation cephalosporins (2.7 fold) and sulfonamides ( Marra et al, 2009 ; Li et al, 2023 ).…”

“…It has also been suggested that this species may stimulate and differentiate Treg cells and thus participate in immune regulation ( Troy and Kasper, 2010 ; Johnson et al, 2015 ; Wu et al, 2021 ). A study by Li et al (2023) , showed that species such as Lactobacillus fermentum and Bacteroides fragilis play a role as probiotic strains. Their combined use in senescent mice improved neuronal cell necrosis, antioxidant capacity, and reduced inflammation levels ( Li et al, 2023 ).…”