Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome

Jansen, Charlotte; Elisen, Marc G.L.M.; Leenstra, Cor W.; Kaaijk, Eugenie M.; Stralen, Karlijn J. van; Verhoeve, Harold R.

doi:10.1016/j.fertnstert.2017.08.003

Cited by 9 publications

(10 citation statements)

References 11 publications

(16 reference statements)

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“…These results are in accordance with Jansen et al [27] who documented that there is no negative effect on pregnancy rate when IUI of processed sperm is delayed until the next day. Moreover, Lee et al [28] reported that insemination at least 36 h after ovulation is associated with increased pregnancy rate compared to IUI performed ≤36 h following ovulation.…”

Section: Discussionsupporting

confidence: 92%

Prospective comparative trial to define the optimum time for insemination during stimulated intrauterine insemination cycles

El-Kallaf

2018

Evidence Based Women's Health Journal

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Objectives: To determine the clinical pregnancy rate (CPR) of women who underwent clomiphene citrate (CC) and human menopausal gonadotropin ovarian stimulation (OS) followed by intrauterine insemination (IUI) at varied times after hCG ovulation triggering and to determine the appropriate IUI timing. Patients and Methods: 152 women with primary infertility secondary to male subfertility were randomly allocated into groups (I-IV) according to timing of IUI at 34-35, 35-36, 36-37, and 37-38hr after ovulation triggering. OS consisted of 5-days oral CC 100 mg once daily since day-3 of the menstrual cycle followed by daily injection of human menopausal gonadotropin 150 IU since day-8 until hCG injection. Ovulation was monitored using TVU for evident ovulation with a dominant follicle size >18 mm. IUI was repeated for three cycles and women were evaluated for clinical pregnancy rate (CPR) and abortion and multiple pregnancy rates were recorded. Results: 44 women had clinical pregnancy for a total CPR of 28.9%. CPR% /patient was 15.8%, 34.2%, 42.1% and 23.7% and CPR%/cycle was 6, 14, 20 and 11 in the four groups, respectively with significant differences in favor of group III. Three women had ectopic and six women had multiple pregnancy and five women had abortion. Statistical analyses defined high number of performed IUI cycles as significantly negative, while longer time till IUI after hCG injection as significantly positive predictor for trial success. Kaplan-Meier regression analysis defined 37 hr (95% CI: 36.5-37.5) after hCG injection as the appropriate time to get the best chance for IUI success. Conclusion: IUI after CC + GN ovarian stimulation is an appropriate policy for infertile couples secondary to male subfertility. Total motile sperm count threshold at ≥5 million gives acceptable outcome. Semen injection at 37 hr after hCG ovulation triggering gives the highest CPR.

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Section: Discussionsupporting

confidence: 92%

Prospective comparative trial to define the optimum time for insemination during stimulated intrauterine insemination cycles

El-Kallaf

2018

Evidence Based Women's Health Journal

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“…In addition, differences in screening/exclusion criteria and a lack of standardized IUI techniques between studies would also bias their comparability. Indeed, differences in sperm quality and processing, number of IUI cycles, cycle timing, and differences in ovarian stimulation have all been shown to affect IUI outcomes . It is also important to note that the control group included couples with an independent condition, unexplained infertility, which may be associated with fertility‐reducing factors that may bias these findings; however, given that all patients undergoing COH‐IUI inevitably have some form of infertility, it remains the most appropriate comparison group as all known and reversible causes of infertility have been screened and excluded.…”

Section: Discussioncontrasting

confidence: 85%

The effect of unilateral tubal block diagnosed by hysterosalpingogram on clinical pregnancy rate in intrauterine insemination cycles: systematic review and meta‐analysis

Tan

Tannus

Taşkın

et al. 2018

BJOG

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“… 48 Therefore, differences in the location of glycocalyx residues during selection/capacitation could explain the lower capacity of spermatozoa to recognize and bind to the zona pellucida when incubated for a relatively short capacitation time (1 h). 16 51 We additionally identified strong lectin pattern correlations during 4 h capacitation, e.g ., P1 in AAA, P2 for ConA, P1 with PNA, and P4 for WGA. The role played by these sugar relocation patterns could be related to proper sperm function and adequate oocyte recognition.…”

Section: Discussionmentioning

confidence: 93%

Influence of in vitro capacitation time on structural and functional human sperm parameters

et al. 2020

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A cascade of dramatic physiological events is linked to the sperm acrosome reaction and binding to the oocyte's zona pellucida during human sperm capacitation. However, structural and functional sperm changes during capacitation currently remain poorly defined. Here, we performed a multibiomarker approach based on the utilization of sperm concentration, motility, viability, morphology, acrosome reaction, tyrosine phosphorylation, DNA fragmentation, and lectin-binding sites to analyze the impact caused by swim-up selection times (uncapacitated, 1 h capacitated, and 4 h capacitated) on sperm function and structure in normozoospermic samples. We found that a 4 h swim-up capacitation increased sperm quality, because a large number of cells with normal morphology and lower DNA fragmentation rates were recovered. Furthermore, the long-term capacitation induced a higher percentage of cells with tyrosine phosphorylation of the principal piece as well as a redistribution of lectin-binding sites. Overall, the multivariate biomarkers analyzed showed a less variable distribution on spermatozoa recovered after 4 h capacitation than that with the shorter capacitation time. These findings stress the importance of capacitation time as a relevant factor in sperm quality with potential biological reproductive implications both for basic research and in assisted reproduction techniques.

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Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome

Abstract: There is no negative effect on pregnancy rate when IUI of processed sperm is delayed until the next day. This approach allows additional flexibility for couples when the male partner is not available on the day of ovulation, and it allows for a spread of workload in the laboratory.

Cited by 9 publications

References 11 publications

Prospective comparative trial to define the optimum time for insemination during stimulated intrauterine insemination cycles

Prospective comparative trial to define the optimum time for insemination during stimulated intrauterine insemination cycles

The effect of unilateral tubal block diagnosed by hysterosalpingogram on clinical pregnancy rate in intrauterine insemination cycles: systematic review and meta‐analysis

Influence of in vitro capacitation time on structural and functional human sperm parameters

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