Long-term whole blood DNA preservation by cost-efficient cryosilicification

Zhou, Liang; Lei, Qi; Guo, Jimin; Gao, Yuanyuan; Shi, Jianjun; Yu, Hong; Yin, Wenxiang; Cao, Jian Xin; Xiao, Botao; Andreo, Jacopo; Ettlinger, Romy; Brinker, C. Jeffrey; Wuttke, Stefan; Wang, Zhu

doi:10.1038/s41467-022-33759-y

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…After decades of research, many sensors for detecting DNA have been developed. At present, the common DNA detection techniques include electrochemical detection, [87] fluorescence detection, [88] piezoelectric crystal detection method, [89] field effect tube detection, [90] thermal detection, [91] and so on. DNA sensors have been widely used in gene detection, cancer detection, and environmental detection, which is of great significance.…”

Section: Detection Of Dnamentioning

confidence: 99%

Chemical Sensors using Single‐Molecule Electrical Measurements

Sun

Xue

et al. 2023

Chemistry An Asian Journal

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Driven by the digitization and informatization of contemporary society, electrical sensors are developing toward minimal structure, intelligent function, and high detection resolution. Single-molecule electrical measurement techniques have been proven to be capable of label-free molecular recognition and detection, which opens a new strategy for the design of efficient single-molecule detection sensors. In this review, we outline the main advances and potentials of single-molecule electronics for qualitative identification and recognition assays at the single-molecule level. Strategies for single-molecule electro-sensing and its main applications are reviewed, mainly in the detection of ions, small molecules, oligomers, genetic materials, and proteins. This review summarizes the remaining challenges in the current development of single-molecule electrical sensing and presents some potential perspectives for this field.

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Section: Detection Of Dnamentioning

confidence: 99%

Chemical Sensors using Single‐Molecule Electrical Measurements

Sun

Xue

et al. 2023

Chemistry An Asian Journal

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“…The first works on the entrapment of biomaterial into a silica matrix using the sol–gel chemistry method revealed a number of important advantages of the approach, which was the reason for increased attention and its rapid development [ 5 , 6 , 7 , 9 , 10 , 14 , 17 , 20 , 21 , 26 , 28 , 30 , 50 , 52 , 79 , 160 , 200 , 205 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 ]. Worthy of mention are the following: Control of the composition, structure, porosity and surface area of the silica matrix; A variety of forms—monoliths, films, (nano) particles, capsules, fibrils, powders; Modification of the silica surface by attaching functional groups, organic substances and polymers; Available cheap commercial precursors; Thermal and chemical resistance of SiO 2 ; Optical transparency of the silica matrix; Protection from toxic chemicals, UV radiation and microorganisms; Increasing the long-term and thermal stability of the immobilized biomaterial in sol–gel matrix; Simple, readily available, and cheap equipment that does not require special precautions.…”

Section: Advantages Of Sol–gel Immobilizationmentioning

confidence: 99%

“…The original version of two-stage immobilization was proposed by Zhou et al [ 219 ]. They prepared a solution of silica sol with whole blood cells, which was frozen at −80 °C.…”

Section: Biomimetic Approachesmentioning

confidence: 99%

“…Immobilization by means of a two-stage scheme has been applied in many laboratories, showing its effectiveness. It was successfully used to entrap alkaline and acid phosphatase [ 217 , 222 , 229 ], bacteriorhodopsin [ 74 ], butyrylcholinesterase [ 218 ], cholesterol oxidase and cholesterol esterase [ 230 ], cyclodextrin [ 231 ], cytochrome c [ 49 , 232 , 233 , 234 ], DNA [ 219 , 235 ], glucose-oxidase [ 184 , 236 , 237 ], horseradish peroxidase [ 87 , 232 , 238 , 239 , 240 ], lipase [ 157 , 241 ], lysozyme [ 82 , 83 , 242 , 243 ], myoglobin [ 49 , 242 ], RNA aptamers [ 66 ] tryptophan [ 244 ], tyrosinase [ 239 ]. In addition, the method has been used to immobilize bacteria [ 88 , 196 , 222 , 224 , 225 , 245 , 246 ], microalgae [ 44 , 227 ], and viruses [ 228 ].…”

Section: Biomimetic Approachesmentioning

confidence: 99%

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Biomimetic Sol–Gel Chemistry to Tailor Structure, Properties, and Functionality of Bionanocomposites by Biopolymers and Cells

Shchipunov

2023

Materials

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Biosilica, synthesized annually only by diatoms, is almost 1000 times more abundant than industrial silica. Biosilicification occurs at a high rate, although the concentration of silicic acid in natural waters is ~100 μM. It occurs in neutral aqueous solutions, at ambient temperature, and under the control of proteins that determine the formation of hierarchically organized structures. Using diatoms as an example, the fundamental differences between biosilicification and traditional sol–gel technology, which is performed with the addition of acid/alkali, organic solvents and heating, have been identified. The conditions are harsh for the biomaterial, as they cause protein denaturation and cell death. Numerous attempts are being made to bring sol–gel technology closer to biomineralization processes. Biomimetic synthesis must be conducted at physiological pH, room temperature, and without the addition of organic solvents. To date, significant progress has been made in approaching these requirements. The review presents a critical analysis of the approaches proposed to date for the silicification of biomacromolecules and cells, the formation of bionanocomposites with controlled structure, porosity, and functionality determined by the biomaterial. They demonstrated the broad capabilities and prospects of biomimetic methods for creating optical and photonic materials, adsorbents, catalysts and biocatalysts, sensors and biosensors, and biomaterials for biomedicine.

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“…Biological evidence requires special storage, preservation and transport conditions as it is more susceptible to damage and contamination than other evidence at the scene of crime. Blood and other body fluids are exposed to contamination with bacteria and other organisms, which exposes them to damage, DNA degradation and mixing it with other sources of DNA [18]. Therefore, the crime scene team should be trained, skilled and well equipped with the necessary tools and equipment.…”

Section: Introductionmentioning

confidence: 99%

Level of Experiences in processing of Biological Forensic Traces at the Crime Scene among Palestinian Police Employees

Khalilia

2024

J Forensic investig

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Crime Scene Investigation (CSI) means taking all lawful methods, processes and procedures that lead to solving crimes and detecting the truth.This study aimed to evaluate the Palestinian Police Agency (PPA) employees' perceptions towards the experiences in the documentation, collection, preservation, packaging and transportation of biological traces from the scene of crime. An item-based questionnaire was designed with this aim in mind, and its reliability as well as validity were tested. The study's target group included (244) PPA-affiliated crime scene investigators and officers who served in the governorates of Hebron, Jenin, and Ramallah during summer of 2022. The majority of respondents expressed agreement or neutrality regarding the technical procedures used at the crime scene. In general, PPA employees have positive experiences with the preservation, documentation, collection, packaging, and transportation of biological evidence from the scene. The study's findings make it abundantly evident that the average for each paragraph was greater than (3.57), demonstrating that PPA personnel working at the crime scene generally had sufficient experience handling forensic traces. However, preservation and collection of biological traces especially DNA sources from the crime scene were the lowest with a total averages of (3.40). While the searching and documentation of the crime scene section, andthe packaging and transportation of the biological traces from the scene of the crime the total averages were (3.69) and (3.62) respectively.In addition, there was a significant difference (P < 0.05) between the respondents regarding to the three working places, age, and ranks. This study recommending the decision makers in Palestine to pay attention to the technical procedures at the crime scene and employ a trained team that has sufficient knowledge and skills to deal with biological forensic traces at the crime scene. This study also recommends conducting deeper analytical studies to include all departments and entities working at crime scenes in all governorates in Palestine.

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Long-term whole blood DNA preservation by cost-efficient cryosilicification

Cited by 10 publications

References 46 publications

Chemical Sensors using Single‐Molecule Electrical Measurements

Chemical Sensors using Single‐Molecule Electrical Measurements

Biomimetic Sol–Gel Chemistry to Tailor Structure, Properties, and Functionality of Bionanocomposites by Biopolymers and Cells

Level of Experiences in processing of Biological Forensic Traces at the Crime Scene among Palestinian Police Employees

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