Long-Term Two-Photon Imaging in Awake Macaque Monkey

Li, Ming; Liu, Fang; Jiang, Hongfei; Lee, Tai Sing; Tang, Shiming

doi:10.1016/j.neuron.2017.01.027

Cited by 109 publications

(140 citation statements)

References 33 publications

(49 reference statements)

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“…We injected AAV1-hSyn-GCaMP into V4 of two rhesus macaques -GCaMP5G for monkey A and GCaMP6s for monkey B. The cranial imaging window and head posts were implanted 1-2 months after viral injection 37 . During experiments, monkeys were required to perform a simple fixation task for 2.5s with head restrained.…”

Section: Resultsmentioning

confidence: 99%

“…To address this question, we performed two-photon calcium imaging in awake macaque cortex. This technique increases the spatial resolution for functional characterization at the single-cell level while enabling the visualization of spatial distribution and clustering within the cortical population [36][37][38] . We recorded the responses of hundreds of V4 neurons to thousands of natural images.…”

Section: Introductionmentioning

confidence: 99%

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Clustered Functional Domains for Curves and Corners in Cortical Area V4

Jiang

Andolina

et al. 2019

Preprint

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As an intermediate stage of the ventral visual pathway, V4 plays a crucial role in transforming basic orientation information into higher-ordered object representations. However, the neural mechanisms underlying the encoding of simple, complex and intermediate features in V4 remain poorly understood.Using two-photon calcium imaging in awake macaques, we recorded the responses of large populations of V4 neurons to thousands of natural images. To understand these high-dimensional data sets, we performed dimensional reduction analysis on the neuronal population responses. We found orthogonal dimensions encoding orientation and more complex features, with curves and corners represented separately. These distinct feature dimensions were encoded by spatially clustered subpopulations of neurons organized in iso-orientation and curvature domains. Using synthetic stimuli, we confirmed that curvature and corner selective neurons in V4 were tuned to integral features rather than combinations of local orientation compartments. Our results show that curves and corners are encoded by neurons clustered into functional domains separate from orientation. This functionally specific population architecture may serve to facilitate local computations that expedite more complex shape and object representations at higher levels of the ventral pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clustered Functional Domains for Curves and Corners in Cortical Area V4

Jiang

Andolina

et al. 2019

Preprint

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“…A T-shaped steel frame was installed for head stabilization, and an optical 17 window was inserted onto the cortical surface. More details of the preparation and 18 surgical procedures can be found in Li et al (2017). The procedures were approved by 19 the Institutional Animal Care and Use Committee at Beijing University.…”

Section: Monkey Preparationmentioning

confidence: 99%

“…Intrinsic 10 optical imaging, on the other hand, lacks cellular resolution. The two-photon imaging 11 technology is able to overcome these difficulties in large measure by simultaneously 12 recording hundreds of neurons' activities at single cell precision (Ohki et al, 2005;13 Nauhaus et al, 2012; Nauhaus et al, 2016;Li et al, 2017). Most relevant to the current 14 study, Nauhaus et al (2012;2016) have studied V1 orientation and spatial frequency 15 tuning in anesthetized macaques, using two-photon imaging with a bulk-loaded calcium 16…”

Section: Introductionmentioning

confidence: 99%

Cellular precision of orientation and spatial frequency maps in macaque V1

Guan

Tang

et al. 2019

Preprint

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9Functional organization of neuronal response properties along the surface of the 10 neocortex is a fundamental guiding principle of neural computation in the brain. Despite 11 this importance, the cellular precision of functional maps is still largely unknown. We 12 address the challenge by using two-photon calcium imaging to measure cell-specific 13 orientation and spatial frequency (SF) responses across fields of macaque V1 superficial 14 layers. The cellular orientation maps confirm iso-orientation domains, but rarely show 15 pinwheels. Pinwheels obtained through conventional Gaussian smoothing and vector 16 summation of orientation responses mostly overlap with blood vessel regions, 17 suggesting false singularities. Cellular SF maps clarify existing controversies by showing 18 weak iso-frequency clusters, which also suggests a weak geometric relationship between 19 orientation and SF maps. Most neurons are tuned to medium frequencies, but the tuning 20

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“…This approach, however, is often challenged by progressive deterioration in optical quality (19)(20)(21). The degradation is observed as a cloudy layer that gradually overtakes the fluid filled gap between the cranial window and the brain tissue, and is thought to arise from the natural inflammatory response that follows a craniotomy (18)(19)(20)(22)(23)(24). As granulation tissue grows, its inhomogeneous structure scatters light at the brain-towindow interface, which consequently degrades optical quality and blurs fluorescence signals.…”

Section: Introductionmentioning

confidence: 99%

Removable cranial window for sustained wide-field optical imaging in mouse neocortex

Susie

Bucklin

Han

2020

Preprint

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A These authors contributed equally to this work Attempts to image neocortical regions on the surface of mouse brain typically use a small glass disc attached to the cranial surface. This approach, however, is often challenged by progressive deterioration in optical quality and permits limited tissue access after its initial implantation. Here we describe a design and demonstrate a two-stage cranial implant device developed with a remarkably versatile material, polydimethylsiloxane, which facilitates longitudinal imaging experiments in mouse cortex. The system was designed considering biocompatibility and optical performance. This enabled us to achieve sustained periods of optical quality, extending beyond a year in some mice, and allows imaging at high spatio-temporal resolution using widefield microscopy. Additionally, the two-part system, consisting of a fixed headplate with integrated neural access chamber and optical insert, allowed flexible access to the underlying tissue offering an expansive toolbox of neuromanipulation possibilities. Finally, we demonstrate the technical feasibility of rapid adaptation of the system to accommodate varying applications requiring long-term ability to visualize and access neural tissue. This capability will drastically reduce wasted time and resources for experiments of any duration, and will facilitate previously infeasible studies requiring long-term observation such as for research in aging or the progression chronic neurological disorders. calcium imaging | chronic cranial window | fluorescence imaging | GCaMP | intravital microscopy | neocortex | silicone implant | surgery | tissue access

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Long-Term Two-Photon Imaging in Awake Macaque Monkey

Cited by 109 publications

References 33 publications

Clustered Functional Domains for Curves and Corners in Cortical Area V4

Clustered Functional Domains for Curves and Corners in Cortical Area V4

Cellular precision of orientation and spatial frequency maps in macaque V1

Removable cranial window for sustained wide-field optical imaging in mouse neocortex

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