Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice

Li, Shu; Lu, Dandan; Zhang, Yaling; Zhang, Yi

doi:10.1007/s10815-013-0102-2

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…Previous studies have reported decreased gene expression of P450 cholesterol side chain cleavage enzyme (CYP11A1), 3β‐hydroxysteroid dehydrogenase (HSD) and steroidogenic factor 1 in Leydig cells exposed to nicotine in vitro (Guo et al., ) and in vivo (Jana et al., ). Apart from oxidative stress which was reported to directly cause testicular impairment and decline in spermatogenesis following nicotine exposure (Jana et al., ; Li et al., ; Mosbah et al., ), reduced circulating levels of FSH and LH may be partly responsible for the observed impairment in spermatogenesis, thus suggesting an extra‐testicular mechanism. Considering the fact that FSH and LH are extra‐testicular hormones, it is likely that the stressors impaired pituitary function.…”

Section: Discussionmentioning

confidence: 99%

“…, ; Simoni, Bakker, & Krausz, ; Rinaldi, Fontani, Aravagli, & Saragò, ) impair fertility, the level of exposure of young adult men of childbearing age to stress and nicotine is on the increase. Several reports have linked stress‐ and nicotine‐induced reproductive impairment to oxidative stress (Jana, Samanta, & De, ; Li, Lu, Zhang, & Zhang, ; Mosbah, Yousef, & Mantovani, ). Vitamin E is one of the antioxidants used over the years for management of male infertility.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin E attenuates nicotine- and noise-induced reproductive impairment in male albino Wistar rats

et al. 2018

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Previous studies showed that exposure to stress or nicotine induced reproductive impairment in male rats. Here, we assessed the effect of an antioxidant (vitamin E) on nicotine-, stress- and nicotine + stress-induced reproductive impairment in male rats. Forty-eight male albino Wistar rats were divided into eight groups as follows; control, stress (generator noise 90-120 dB, 8 hr/day), nicotine (1.5 mg kg day ), nicotine + stress, vitamin E (100 mg kg day ), stress + vitamin E, nicotine + vitamin E and stress + nicotine + vitamin E. Sperm count, viability, motility and rapid progressive forward movement decreased significantly (p < 0.05), while percentage of nonmotile spermatozoa increased significantly (p < 0.05) in stress, nicotine and nicotine + stress groups, compared with control. Serum testosterone and follicle-stimulating hormone decreased significantly (p < 0.05) in stress, nicotine and nicotine + stress groups, compared with control. Serum luteinising hormone decreased (p < 0.05) significantly in stress and nicotine + stress groups, compared with the control. Histology of the testes showed loss of germ cells in numerous seminiferous tubules, and epididymal histology showed decreased sperm density in stress, nicotine and nicotine + stress groups compared with the control. These negative changes were more severe in the nicotine + stress group. Vitamin E ameliorated the negative changes in the above parameters. This may be attributable to its antioxidant property.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vitamin E attenuates nicotine- and noise-induced reproductive impairment in male albino Wistar rats

et al. 2018

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“…Since the hydrogen molecule is electrically neutral and considerably smaller than other antioxidants, it can easily penetrate membranes and enter organelles (12,26); however, it remains unclear exactly what type of oxidative stress is involved in femoral head necrosis. GSH stabilizes the lysosomal membranes to suppress injury to the vascular endothelium.…”

Section: A B a B Discussionmentioning

confidence: 99%

“…Hydrogen has a positive effect in inhibiting oxidative stress. Hydrogen gas is flammable, explosive and difficult to store and use; however, hydrogen gas-saturated saline, also known as hydrogen-rich saline, is safe, economical and easily available, and is universally employed in medicine (12,13). Injecting hydrogen-rich saline into the intraperitoneal cavity is an easy and effective method that can be readily adapted for potential clinical practice (14).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model

Huang

Jiao

Yan

2015

Experimental and Therapeutic Medicine

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Abstract.A growing body of evidence suggests that hydrogen is a novel, selective antioxidant that exerts a protective effect against organ damage. The present study investigated the effect of hydrogen-rich saline on corticosteroid-induced necrosis of the femoral head in an animal model established using prednisolone. A total of 30 healthy, male, adult New Zealand white rabbits were randomly divided into two groups: Hydrogen-rich saline (treated with hydrogen-rich saline via intraperitoneal injection) and placebo (treated with normal saline). At the set time-points, the structure of the femoral head was examined using a microscope; the concentrations of glutathione (GSH), lipid peroxide (LPO), vascular endothelial growth factor (VEGF) and thrombomodulin (TM) in the plasma were measured and the microvessel density was quantified. The results showed that hydrogen-rich saline significantly decreased the levels of VEGF, TM and LPO and increased the GSH level in steroid-associated necrosis of the femoral head in the rabbit model. A significant increase in the microvessel density was observed in the hydrogen-rich saline group. Histopathological staining confirmed the results of the biochemical analysis. The present study demonstrates that hydrogen treatment may alleviate steroid-associated osteonecrosis by inhibiting oxidative stress. Hydrogen-rich saline may provide an alternative treatment for steroid-associated necrosis of the femoral head.

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“…Apoptosis can seriously affect reproductive function of normal testes. Nicotine is also reported to enhance the synthesis of cholesterol, triglycerides, phospholipids and free fatty acids in the testes and increase peroxidative damage (Li, Lu, & Zhang, ). On the other hand, statins are prescribed to reduce hyperlipidaemia (Tobert, ).…”

Section: Introductionmentioning

confidence: 99%

Atorvastatin modulates drug transporters and ameliorates nicotine-induced testicular toxicity

2018

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We studied the changes in mRNA expressions of influx and efflux transporters, blood-testis-barrier proteins (BTB) and key apoptotic genes in the testis of rats coadministered with nicotine and atorvastatin. Rats were divided into four groups: (i) control, (ii) atorvastatin (10 mg/kg b.wt), (iii) nicotine (0.6 mg/kg b.wt) and (iv) atorvastatin (10 mg/kg b.wt) + nicotine (0.6 mg/kg b.wt). Atorvastatin was given by oral intubation and nicotine by intraperitoneal injection. After 60 days of treatment, expressions of key apoptotic genes involved in both intrinsic and extrinsic pathways; solute carrier influx transporters SLCOB1, SLC22A1 and efflux transporter ABCB1 associated with transport of atorvastatin and nicotine, and proteins of BTB were assayed. Nicotine administration activated apoptosis and downregulated SLCOB1, which transport atorvastatin. Atorvastatin administration suppressed apoptotic pathway and downregulated SLC22A1, transporter of nicotine. Coadministration of atorvastatin with nicotine downregulated expressions of apoptotic genes. The combined administration of atorvastatin and nicotine reduced the influx of both atorvastatin and nicotine and enhanced the efflux of these drugs thereby altering the microenvironment of testis and improving testicular function. We conclude that atorvastatin-mediated alterations of BTB and drug transporters might have played a significant role in ameliorating nicotine-induced testicular toxicity.

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Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice

Cited by 17 publications

References 27 publications

Vitamin E attenuates nicotine- and noise-induced reproductive impairment in male albino Wistar rats

Vitamin E attenuates nicotine- and noise-induced reproductive impairment in male albino Wistar rats

Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model

Atorvastatin modulates drug transporters and ameliorates nicotine-induced testicular toxicity

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