Long‐term testosterone depletion attenuates inflammatory bone resorption in the ligature‐induced periodontal disease model

Gonçalves, Vanda Maria Gimenes; Ortega, Adriana Alicia C.; Steffens, João Paulo; Spolidório, Denise Madalena Palomari; Rossa, Carlos; Spolidório, Luís Carlos

doi:10.1002/jper.17-0457

Cited by 17 publications

(9 citation statements)

References 42 publications

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“…Some affirmed that both excessive high- and low-level testosterone accelerate alveolar bone loss in rats with periodontitis [ 20 ]. However, several rodent models demonstrated that depletion of testosterone weakens ligature-induced periodontal bone resorption [ 21 , 22 ]. To sum up, excessive testosterone is universally accepted to be detrimental to periodontal health but the effect of testosterone deficiency on periodontal tissue is still under debate.…”

Section: Hormonesmentioning

confidence: 99%

Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review

Zhu

Zhou

Chen

et al. 2022

Cells

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Periodontitis is a periodontal inflammatory condition that results from disrupted periodontal host–microbe homeostasis, manifested by the destruction of tooth-supporting structures, especially inflammatory alveolar bone loss. Osteoporosis is characterized by systemic deterioration of bone mass and microarchitecture. The roles of many systemic factors have been identified in the pathogenesis of osteoporosis, including endocrine change, metabolic disorders, health-impaired behaviors and mental stress. The prevalence rate of osteoporotic fracture is in sustained elevation in the past decades. Recent studies suggest that individuals with concomitant osteoporosis are more vulnerable to periodontal impairment. Current reviews of worse periodontal status in the context of osteoporosis are limited, mainly centering on the impacts of menopausal and diabetic osteoporosis on periodontitis. Herein, this review article makes an effort to provide a comprehensive view of the relationship between osteoporosis and periodontitis, with a focus on clarifying how those risk factors in osteoporotic populations modify the alveolar bone homeostasis in the periodontitis niche.

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Section: Hormonesmentioning

confidence: 99%

Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review

Zhu

Zhou

Chen

et al. 2022

Cells

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“…The sham procedure was performed in 10 rats through testicle exposure and manipulation (without removal), replacement, and suturing (Sham group). After the procedure, the animals were kept in separate cages for recovery for 1 week [20] (Figure 1).…”

Section: Androgen Deprivation Modelmentioning

confidence: 99%

“…Thirty days after surgery, the rats (OCX þ T group) received testosterone undecanoate (Nebido V R intramuscularly, 62.5 mg/kg body weight -Bayer, Socorro, Brazil). On day 60, a second exogenous testosterone administration was performed until day 77, as previously described, for the maintenance of physiological levels of testosterone [20,21] (Figure 1).…”

Section: Hormonal Replacement With Testosteronementioning

confidence: 99%

“…The blood samples were centrifuged at 3.000 rpm for 10 min, and the serum was collected and kept at À80 C until the analysis. Each serum sample was analyzed for total testosterone levels using a chemiluminescence-based immunoassay, and the concentration was expressed in ng/mL (Immulite 2000, Siemens Healthcare Diagnostics, Gwynedd, United Kingdom) [20]. The purpose of the second and third harvestings was to verify the pharmacokinetics of the exogenous testosterone administered, comparing the hormone concentration in this group with the sham and orchiectomized animals.…”

Section: Serum Testosterone Evaluationmentioning

confidence: 99%

“…The phantom rods were scanned and reconstructed using the same parameters as those used for the femur, as described above. The attenuation coefficient was entered into the calibration dialogue window in CTAn to determine BMD (mg/cm 3 ) [19,20,22].…”

Section: Microcomputed Tomography (Mct) Analysismentioning

confidence: 99%

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Physiological testosterone replacement effects on male aged rats with orchiectomy-induced osteoporosis in advanced stage: a tomographic and biomechanical pilot study

et al. 2021

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Aim: This study aimed to evaluate the effect of physiological testosterone replacement on male aged rats with orchiectomy-induced osteoporosis in advanced stage. Methods: Thirty male rats (Rattus norvegicus albinus, Holtzman lineage) were randomly distributed into 3 groups (n ¼ 10): 1-sham, 2-orchiectomy (OCX), 3-OCX þ testosterone replacement (OCX þ T). On day 0, a sham or orchiectomy surgery was performed according to the groups. Thirty and sixty days after surgeries, the animals from OCX þ T group received testosterone intramuscularly, and the rats in all groups were euthanized on day 77. The femurs were removed for micro-CT scanning and biomechanical test. Results: Orchiectomy resulted in a marked trabecular bone damage (p < 0.05), which was not reversed with testosterone treatment (OCX þ T group). The femoral strength was lower in orchiectomized animals (p < 0.05), while the bone strength in OCX þ T group was similar to that observed in the sham animals (p > 0.05) and correlated to this parameter the deformation of rupture was smaller in OCX þ T group. Conclusion:In conclusion, testosterone depletion induced by orchiectomy established an osteoporotic environment, mainly affecting the trabecular bone. Moreover, even though testosterone treatment did not enhance these variables, the hormonal replacement improved the femoral fracture strength and promoted beneficial effects on the biomechanical parameters compromised by castration in femoral bone.

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Role of testosterone and androgen receptor in periodontal disease progression in female rats

Steffens

Valenga

Santana

et al. 2019

Journal of Periodontology

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Background: Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females. Herein, we investigated the role of androgen receptor and TES on the inflammatory response and alveolar bone resorption associated with ligature-induced periodontal disease in female rats.Methods: Fifty female Holtzman rats were divided in five groups (n = 10/group):androgen receptor antagonist (flutamide); estrogen receptor antagonist (fulvestrant); TES supplementation; aromatase inhibitor (anastrozole); and TES plus anastrozole.Periodontitis was induced by ligatures around the lower first molars for 2 weeks. Twenty animals (n = 10/group) were used as untreated ligated or non-ligated controls.Bone loss and the number of osteoclasts were measured through radiographic and immunohistochemical analysis, respectively. Inflammatory cytokines, chemokines and bone markers were measured by multiplex immunoassay and ELISA in serum samples and periodontal tissues. Results:The blockage of androgen receptor significantly increased radiographic bone loss and tissue levels of IL-1 (P <0.05), IL-1 (P <0.001) and IL-10 (P <0.01) compared with the periodontitis group. Testosterone supplementation significantly increased EGF levels in tissue samples, whereas when combined with aromatase inhibitor anastrozole significantly increased both EGF and VEGF (P <0.05). None of the treatment conditions significantly impacted the number of osteoclasts compared with the periodontitis group. Conclusions:Androgen receptor activation is an important factor in the regulation of several inflammatory markers, and its blockage significantly increases bone loss. K E Y W O R D Salveolar bone loss, androgen receptor antagonists, aromatase inhibitors, estrogen antagonists, inflammation, testosterone J Periodontol. 2020;91:545-553.

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Long‐term testosterone depletion attenuates inflammatory bone resorption in the ligature‐induced periodontal disease model

Abstract: The findings of the present study suggest that testosterone depletion attenuates inflammatory bone resorption in ligature-induced periodontitis, which may be partly mediated via decreased production of IL-1β.

Cited by 17 publications

References 42 publications

Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review

Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review

Physiological testosterone replacement effects on male aged rats with orchiectomy-induced osteoporosis in advanced stage: a tomographic and biomechanical pilot study

Role of testosterone and androgen receptor in periodontal disease progression in female rats

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