Long-term, synergistic and high-efficient antibacterial polyacrylonitrile nanofibrous membrane prepared by “one-pot” electrospinning process

Ultraporous Polyquaternium-Carboxylated Chitosan Composite Hydrogel Spheres with Anticoagulant, Antibacterial, and Rapid Endotoxin Removal Profiles for Sepsis Treatment

“…Mueller Hinton agar (MHA) and Mueller Hinton broth (MHB) were used as culture media. A total of 200 mg of hydrogel spheres was added to 1 mL of 10 6 CFU/mL bacterial suspension and cocultured with shaking at 37 °C for 24 h. The bacterial suspension after coculturing was diluted and counted through the spread plate method to calculate the antibacterial ratios . The sterilization experiment of the hydrogel spheres was performed by coculturing 10 8 CFU/mL of bacterial suspension with 200 mg/mL of hydrogel spheres for 24 h. Then, the bacterial suspension was stained with SYTO 9 and propidium iodide (Thermo Fisher LIVE/DEAD Bac Light Bacterial Viability Kit) to observe live/dead bacteria under a fluorescence microscope (OLYPUS IX53, Japan).…”

“…A total of 200 mg of hydrogel spheres was added to 1 mL of 10 6 CFU/mL bacterial suspension and cocultured with shaking at 37 °C for 24 h. The bacterial suspension after coculturing was diluted and counted through the spread plate method to calculate the antibacterial ratios. 35 The sterilization experiment of the hydrogel spheres was performed by coculturing 10 8 CFU/mL of bacterial suspension with 200 mg/mL of hydrogel spheres for 24 h. Then, the bacterial suspension was stained with SYTO 9 and propidium iodide (Thermo Fisher LIVE/DEAD BacLight Bacterial Viability Kit) to observe live/dead bacteria under a fluorescence microscope (OLYPUS IX53, Japan). For the sketching of the realtime against bacterial growth curve, 10 6 CFU/mL of bacterial suspension was cocultured with 200 mg/mL of hydrogel spheres, and a UV−vis spectrophotometer was employed to measure the optical density (OD) values of the bacterial suspension at 600 nm every 2 h for 12 h.…”

A Straightforward Approach towards Antibacterial and Anti‐Inflammatory Multifunctional Nanofiber Membranes with Sustained Drug Release Profiles

“…To confirm the successful synthesis of PHGC, 1 H and 13 C nuclear magnetic resonance (NMR) spectra of the product in D 2 O solution were acquired on an NMR spectrometer (AV III HD 400 MHz, Bruker, Germany). Infrared spectra of the PHGC were obtained using a Fourier Transform Infrared spectrometer (FTIR, Thermo Scientific Nicolet iS50, USA).…”

“…[5][6][7][8][9] To prevent wounds from bacterial infection, antibacterial agents such as antibiotics, antimicrobial polymers, and nanoparticles (NPs) like silver NPs, have been widely loaded into electrospun wound dressings. [10][11][12][13][14] Among them, antibacterial polymers are featured for their low biotoxicity, long-acting antibacterial activity, prevention of antibiotic resistance, and feasibility of functional modification. [15] Especially, polyhexamethylene guanidine hydrochloride (PHGC) and its derivatives, with similar structures to natural antibacterial peptides, stand out because of their high antibacterial efficiency and excellent biocompatibility.…”

Electrospun polyacrylonitrile/hydroxyapatite composite nanofibrous membranes for the removal of lead ions from aqueous solutions

“…[25][26][27][28][29][30] Polyacrylonitrile (PAN) has been widely used as an electrospun membrane because of its good spinnability, low toxicity, and chemical stability. 31 Besides this, the plentiful cyanogen (-CN) groups in its structure allow PAN to be easily modified. 27 However, PAN has a low adsorption capacity towards heavy metals.…”