Long term survival and limited migration of genetically modified monocytes/macrophages grafted into the mouse brain

Sarkis, Chamsy; Gras, Gabriel; Sanchez, Françoise; Mallet, Jacques; Serguera, Ché

doi:10.4236/jbise.2013.65071

Cited by 1 publication

(1 citation statement)

References 41 publications

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“…The cell surface is made of a diversity of biomolecules that govern the biological processes of the cell, such as cell signaling, cell–cell adhesions, and other extracellular/intracellular communications. Cell surface re-engineering with biologically important molecules has scope for potential applications such as cell labeling, imaging, and functionalization. , In general, direct addition of biological functionality onto live cell surfaces allows for the molecular level analysis of cell surface phenomena and manipulation of cell functions as well. In addition, introduction of chemical reactive groups at the cell surface enables rapid and site-specific cell surface labeling and modification opportunity, providing enormous capability to study the cell surface molecular structure and function and the molecular mechanism it underlies.…”

Section: Introductionmentioning

confidence: 99%

Chemical Reactive Anchoring Lipids with Different Performance for Cell Surface Re-engineering Application

et al. 2018

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Introduction of selectively chemical reactive groups at the cell surface enables site-specific cell surface labeling and modification opportunity, thus facilitating the capability to study the cell surface molecular structure and function and the molecular mechanism it underlies. Further, it offers the opportunity to change or improve a cell’s functionality for interest of choice. In this study, two chemical reactive anchor lipids, phosphatidylethanolamine–poly(ethylene glycol)–dibenzocyclooctyne (DSPE–PEG2000–DBCO) and cholesterol–PEG–dibenzocyclooctyne (CHOL–PEG2000–DBCO) were synthesized and their potential application for cell surface re-engineering via lipid fusion were assessed with RAW 264.7 cells as a model cell. Briefly, RAW 264.7 cells were incubated with anchor lipids under various concentrations and at different incubation times. The successful incorporation of the chemical reactive anchor lipids was confirmed by biotinylation via copper-free click chemistry, followed by streptavidin-fluorescein isothiocyanate binding. In comparison, the cholesterol-based anchor lipid afforded a higher cell membrane incorporation efficiency with less internalization than the phospholipid-based anchor lipid. Low cytotoxicity of both anchor lipids upon incorporation into the RAW 264.7 cells was observed. Further, the cell membrane residence time of the cholesterol-based anchor lipid was evaluated with confocal microscopy. This study suggests the potential cell surface re-engineering applications of the chemical reactive anchor lipids.

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