Long-Term Storage Stability and Nitric Oxide Release Behavior of (N-Acetyl-S-nitrosopenicillaminyl)-S-nitrosopenicillamine-Incorporated Silicone Rubber Coatings

Kumar, R.; Chug, Manjyot Kaur; Brisbois, Elizabeth J.

doi:10.1021/acsami.2c06712

Cited by 6 publications

(8 citation statements)

References 63 publications

(128 reference statements)

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“…Aliquots of each formulation were transferred into an amber glass reaction cell in a 37 °C water bath. NO emission was monitored via chemiluminescence using a Sievers 280i Nitric Oxide Analyzer over an 8.0 h period after reconstitution …”

Section: Methodsmentioning

confidence: 99%

“…NO emission was monitored via chemiluminescence using a Sievers 280i Nitric Oxide Analyzer over an 8.0 h period after reconstitution. 62 …”

Section: Methodsmentioning

confidence: 99%

“…NO emission was monitored via chemiluminescence using a Sievers 280i Nitric Oxide Analyzer over an 8.0 h period after reconstitution. 62 Four hours after the last treatment with GSNO, 100 μL of sterile PBS was added to the apical surface of the cell cultures and incubated for 15 min in a cell culture incubator. The apical wash was collected, homogenized, and centrifuged, and the supernatant was used for estimating the NO content as nitrite by HPLC combined with chemiluminescence detection.…”

Section: ■ Introductionmentioning

confidence: 99%

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S-Nitrosoglutathione Reduces the Density of Staphylococcus aureus Biofilms Established on Human Airway Epithelial Cells

et al. 2022

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Patients with chronic rhinosinusitis (CRS) often show persistent colonization by bacteria in the form of biofilms which are resistant to antibiotic treatment. One of the most commonly isolated bacteria in CRS is Staphylococcus aureus (S. aureus). Nitric oxide (NO) is a potent antimicrobial agent and disperses biofilms efficiently. We hypothesized that S-nitrosoglutathione (GSNO), an endogenous NO carrier/donor, synergizes with gentamicin to disperse and reduce the bacterial biofilm density. We prepared GSNO formulations which are stable up to 12 months at room temperature and show the maximum amount of NO release within 1 h. We examined the effects of this GSNO formulation on the S. aureus biofilm established on the apical surface of the mucociliary-differentiated airway epithelial cell cultures regenerated from airway basal (stem) cells from cystic fibrosis (CF) and CRS patients. We demonstrate that for CF cells, which are defective in producing NO, treatment with GSNO at 100 μM increased the NO levels on the apical surface and reduced the biofilm bacterial density by 2 log units without stimulating pro-inflammatory effects or inducing epithelial cell death. In combination with gentamicin, GSNO further enhanced the killing of biofilm bacteria. Compared to placebo, GSNO significantly increased the ciliary beat frequency (CBF) in both infected and uninfected CF cell cultures. The combination of GSNO and gentamicin also reduced the bacterial density of biofilms grown on sinonasal epithelial cells from CRS patients and improved the CBF. These findings demonstrate that GSNO in combination with gentamicin may effectively reduce the density of biofilm bacteria in CRS patients. GSNO treatment may also enhance the mucociliary clearance by improving the CBF.

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Section: Methodsmentioning

confidence: 99%

“…NO emission was monitored via chemiluminescence using a Sievers 280i Nitric Oxide Analyzer over an 8.0 h period after reconstitution. 62 …”

Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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S-Nitrosoglutathione Reduces the Density of Staphylococcus aureus Biofilms Established on Human Airway Epithelial Cells

et al. 2022

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“…S -Nitroso- N -acetylpenicillamine (SNAP) and S -nitrosoglutathione (GSNO) are two commonly used NO donor species that have been studied for biomaterial applications because of their long-term stability and NO release properties in addition to ease of synthesis, low cost, and excellent biocompatibility (Figure f,g) . Other RSNOs such as S -nitroso- N -acetylcysteine (SNACET) (Figure h) and derivatized molecules such as N -acetyl- S -nitrosopenicillaminyl)- S -nitrosopenicillamine (SNAP-SNAP) have also been synthesized and reported. , NO donors can be incorporated into a polymer matrix via solvent impregnation, non-covalent dispersion, blending of the donor in a polymer, or by covalent immobilization of the NO donor moiety to the polymer backbone (Figure ).…”

Section: Advancements In Nitric Oxide-releasing Multifunctional Biome...mentioning

confidence: 99%

“…156 Other RSNOs such as Snitroso-N-acetylcysteine (SNACET) (Figure 10h) and derivatized molecules such as N-acetyl-S-nitrosopenicillaminyl)-Snitrosopenicillamine (SNAP-SNAP) have also been synthesized and reported. 157,158 NO donors can be incorporated into a polymer matrix via solvent impregnation, non-covalent dispersion, blending of the donor in a polymer, or by covalent immobilization of the NO donor moiety to the polymer backbone (Figure 11).…”

Section: Advancements In Nitric Oxide-releasing Multifunctional Biome...mentioning

confidence: 99%

Recent Developments in Multifunctional Antimicrobial Surfaces and Applications toward Advanced Nitric Oxide-Based Biomaterials

Chug

Brisbois

2022

ACS Mater. Au

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Implant-associated infections arising from biofilm development are known to have detrimental effects with compromised quality of life for the patients, implying a progressing issue in healthcare. It has been a struggle for more than 50 years for the biomaterials field to achieve long-term success of medical implants by discouraging bacterial and protein adhesion without adversely affecting the surrounding tissue and cell functions. However, the rate of infections associated with medical devices is continuously escalating because of the intricate nature of bacterial biofilms, antibiotic resistance, and the lack of ability of monofunctional antibacterial materials to prevent the colonization of bacteria on the device surface. For this reason, many current strategies are focused on the development of novel antibacterial surfaces with dual antimicrobial functionality. These surfaces are based on the combination of two components into one system that can eradicate attached bacteria (antibiotics, peptides, nitric oxide, ammonium salts, light, etc.) and also resist or release adhesion of bacteria (hydrophilic polymers, zwitterionic, antiadhesive, topography, bioinspired surfaces, etc.). This review aims to outline the progress made in the field of biomedical engineering and biomaterials for the development of multifunctional antibacterial biomedical devices. Additionally, principles for material design and fabrication are highlighted using characteristic examples, with a special focus on combinational nitric oxide-releasing biomedical interfaces. A brief perspective on future research directions for engineering of dual-function antibacterial surfaces is also presented.

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Hydrogels for Gasotransmitter Delivery: Nitric Oxide, Carbon Monoxide, and Hydrogen Sulfide

Sarkar

Kumar

Matson

2023

Macromolecular Bioscience

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Gasotransmitters, gaseous signaling molecules including nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S), maintain myriad physiological processes. Low levels of gasotransmitters are often associated with specific problems or diseases, so NO, CO, and H2S hold potential in treating bacterial infections, chronic wounds, myocardial infarction, ischemia, and various other diseases. However, their clinical applications as therapeutic agents are limited due to their gaseous nature, short half‐life, and broad physiological roles. One route toward the greater application of gasotransmitters in medicine is through localized delivery. Hydrogels are attractive biomedical materials for the controlled release of embedded therapeutics as they are typically biocompatible, possess high water content, have tunable mechanical properties, and are injectable in certain cases. Hydrogel‐based gasotransmitter delivery systems began with NO, and hydrogels for CO and H2S have appeared more recently. In this review, the biological importance of gasotransmitters is highlighted, and the fabrication of hydrogel materials is discussed, distinguishing between methods used to physically encapsulate small molecule gasotransmitter donor compounds or chemically tether them to a hydrogel scaffold. The release behavior and potential therapeutic applications of gasotransmitter‐releasing hydrogels are also detailed. Finally, the authors envision the future of this field and describe challenges moving forward.

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Long-Term Storage Stability and Nitric Oxide Release Behavior of (N-Acetyl-S-nitrosopenicillaminyl)-S-nitrosopenicillamine-Incorporated Silicone Rubber Coatings

Cited by 6 publications

References 63 publications

S-Nitrosoglutathione Reduces the Density of Staphylococcus aureus Biofilms Established on Human Airway Epithelial Cells

S-Nitrosoglutathione Reduces the Density of Staphylococcus aureus Biofilms Established on Human Airway Epithelial Cells

Recent Developments in Multifunctional Antimicrobial Surfaces and Applications toward Advanced Nitric Oxide-Based Biomaterials

Hydrogels for Gasotransmitter Delivery: Nitric Oxide, Carbon Monoxide, and Hydrogen Sulfide

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