Long-term stable bioprocess-derived Pickering-type emulsions: Identification of key parameters for emulsion stability based on cell interaction at interface

Janssen, Lisa; Sadowski, Gabriele; Brandenbusch, Christoph

doi:10.1016/j.ces.2022.118164

Cited by 4 publications

(23 citation statements)

References 32 publications

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“…Process is monitored by measuring electrical conductivity κ (left) and determination of the volumetric phase ratio V o :V w (right) in the mixer. Solid lines illustrate the critical phase ratios (V o :V w ) determined previously [ 5 ] . Dotted lines illustrate the (actual/safe) values of V o :V w set for APCI as described previously (section 3.2).…”

Section: Resultsmentioning

confidence: 97%

“…Characterizing the phase inversion process in the presence of cells is crucial to define operating conditions and a process window for the ACPI prototype. The critical volumetric phase ratios V o :V w , required for phase inversion of the bioprocess derived Pickering‐type emulsions processed within this work (either stabilized by E. coli JM101 or P. putida KT2440 cells) to occur, were determined experimentally in a previous work [ 5 ] and are listed in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…[18] Moreover, multiple emulsions (e.g., o/w/o) are formed during the CPI process due to the addition of dispersed phase resulting in an increase of average dispersed phase drop size when approaching the CPI-point. [17] Various batch experiments [5] already revealed the applicability of CPI as a tool for phase separation of various bioprocess-derived Pickering-type emulsions. Further, previous investigations within our group [5] also revealed, that the mechanisms of emulsions stabilization and destabilizing can be attributed/described by three key parameters, namely the particle size R, the three-phase contact angle Θ ow , and the interfacial tension γ ow .…”

Section: Emulsion Phase Separation Using Cpimentioning

confidence: 99%

“…As due to their wetting characteristics (Θ ow = 94 • [5] ) second fraction cells can also stabilize w/o-emulsion type, no phase separation is achieved. Same results were obtained using volumetric phase ratios…”

Section: Steady State Processingmentioning

confidence: 99%

“…[4] Emulsion characteristics depend on the volumetric phase ratio between aqueous and organic phase (V o :V w ) and the wetting behavior of the cells to form o/w-(more hydrophilic cells) and w/o-type (more hydrophobic) emulsions. [5] In some cases also double or multiple emulsions can occur. [6,7] As much as emulsification is desired during the upstream processing (yielding in high surface areas and thus high mass transport rates), [1,3,8,9] downstream processing concepts often fail in processing the resulting (highly stable) Pickering-type emulsions.…”

Section: Pickering-type Emulsions As Limitation In Biphasic Whole-cel...mentioning

confidence: 99%

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Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion

Janssen

Sadowski

Brandenbusch

2023

Biotechnology Journal

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The main bottleneck for the industrial implementation of highly promising multi-phase whole-cell biocatalytic processes is the formation of stable Pickering-type emulsions, hindering efficient downstream processing. Especially for the crucial step of phase separation, state-of-the-art processes require time-consuming and costly process steps (excessive centrifugation/use of de-emulsifiers). In contrast, using the phenomenon of catastrophic phase inversion (CPI), efficient phase separation can be achieved by addition of an excess dispersed phase within minutes. To show applicability of CPI as an innovative process step, a fully automated lab-scale prototype was designed and constructed within this work. A simple mixer-settler set-up enabled a continuous phase separation using CPI termed applied catastrophic phase inversion (ACPI). Test runs were conducted using emulsions from biphasic whole-cell biocatalysis (Escherichia coli JM101 and Pseudomonas putida KT2440 cells). Solvents used included n-heptane, ethyl oleate or 1-octanol as organic phase. These investigations revealed ideal process settings for a stable ACPI process (e.g., flow/stirring rates and volumetric phase ratios between organic and water phase). The knowledge of the CPI point is most crucial, as only the inverted state of emulsion is successfully destabilized.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Emulsion Phase Separation Using Cpimentioning

confidence: 99%

Section: Steady State Processingmentioning

confidence: 99%

Section: Pickering-type Emulsions As Limitation In Biphasic Whole-cel...mentioning

confidence: 99%

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Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion

Janssen

Sadowski

Brandenbusch

2023

Biotechnology Journal

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Tailored Particle Catalysts for Multistep One‐pot Chemoenzymatic Cascade in Pickering Emulsions

Wang

Scandurra

et al. 2022

ChemCatChem

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Chemoenzymatic cascades are an important tool for advanced synthesis in chemistry. However, these cascades are often limited due to the incompatibility issue between two distinct catalysts and reactions. To address this issue, we present a simple multistep one-pot platform, in which nanoparticle catalysts are prepared to allow chemo-and biocatalytic reactions performed sequentially in water and Pickering emulsions. The preparation of particle catalysts is accomplished in just two steps by polymer modifications and [RuCl 2 (pcymene)] 2 coordination, while the benefits of using them for chemoenzymatic synthesis are multifaceted. They act not only as asymmetric catalysts for asymmetric transfer hydrogenation from acetophenone to 1-phenylethanol in water with up to 99 % conversion and 93 % ee, but also as an emulsifier to form stable Pickering emulsions. By the addition of Candida antarctica lipase B into the emulsions, the second-step reaction of enantioselective acylation was achieved with 38 % conversion and 99 % ee. Therefore, we successfully present a simple method to enable chemoenzymatic cascades by combining particle catalysts and enzymes in water and Pickering emulsions in a sequential fashion, which can be generalized for other cascade syntheses with different chemo-and biocatalysts in the future.

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Preparation of curcumin-loaded pectin-nisin copolymer emulsion and evaluation of its stability

Cheng,

Lin,

Zou

et al. 2024

International Journal of Biological Macromolecules

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Long-term stable bioprocess-derived Pickering-type emulsions: Identification of key parameters for emulsion stability based on cell interaction at interface

Cited by 4 publications

References 32 publications

Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion

Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion

Tailored Particle Catalysts for Multistep One‐pot Chemoenzymatic Cascade in Pickering Emulsions

Preparation of curcumin-loaded pectin-nisin copolymer emulsion and evaluation of its stability

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