Long-term stability studies on protection against Newcastle disease by commercial live vaccine used in Brazil

Orsi, Maria Ângela; Zaroni, M. M. H.; Júnior, Luciano Doretto; Camillo, S.C.A; Ribeiro, Simone Alves Mendes; Spilki, Fernando Rosado; Buzinaro, Maria da Glória; Arns, Clarice Weis

doi:10.1016/j.biologicals.2009.04.001

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…1 log 10 EID 50 [37]). It should be considered that in this study all powders were spray dried at the same process parameters and the titre loss during spray drying could be decreased further by optimizing the spray dry process.…”

Section: Discussionmentioning

confidence: 99%

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Huyge

Reeth

Beer

et al. 2012

European Journal of Pharmaceutics and Biopharmaceutics

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Dry powders containing a live-attenuated Newcastle disease vaccine (LZ58 strain) and intended for mass vaccination of poultry were prepared by spray drying using mannitol in combination with trehalose or inositol, polyvinylpyrrolidone (PVP) and/or bovine serum albumin (BSA) as stabilizers. These powders were evaluated for vaccine stabilizing capacity during production and storage (at 6ºC and 25ºC), moisture content, hygroscopicity and dry powder dispersibility. A mixture design, varying the ratio of mannitol, inositol and BSA, was used to select the stabilizer combination which resulted in the desired powder properties (i.e. good vaccine stability during production and storage, low moisture content and hygroscopicity and good dry-dispersibility). Inositol-containing powders had the same vaccine stabilizing capacity as trehalose powders, but were less hygroscopic. Incorporation of BSA enhanced the vaccine stability in the powders compared to PVP-containing formulations. However, increasing the BSA concentration increased the hygroscopicity and reduced the dry dispersibility of the powder. No valid mathematical model could be calculated for vaccine stability during production or storage, but the individual experiments indicated that a formulation combining mannitol, inositol and BSA in a ratio of 73.3:13.3:13.3 (wt/wt) resulted in the lowest vaccine titre loss during production (1.6-2.0 log 10 50% egg infectious dose (EID 50 ) and storage at 6ºC (max. 0.8 log 10 EID 50 after 6 months) in combination with a low moisture content (1.1-1.4%), low hygroscopicity (1.9-2.1% water uptake at 60% relative humidity) and good dry-dispersibility properties.

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Section: Discussionmentioning

confidence: 99%

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Huyge

Reeth

Beer

et al. 2012

European Journal of Pharmaceutics and Biopharmaceutics

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Electrochemical behavior of Newcastle virus La Sota strain (Newvac vaccine) in the presence of gold nanoparticles as sensitizers investigated on Au electrode

Tutunaru

2024

International Journal of Electrochemical Science

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Thermal Stability Study of Five Newcastle Disease Attenuated Vaccine Strains

Boumart

Hamdi²,

Daouam³

et al. 2016

Avian Diseases

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Newcastle disease (ND) is a big concern throughout the world because of the devastating losses that can occur with commercial and backyard poultry. The major problem in many countries is the loss of the vaccine's effectiveness due to inadequate use or storage conditions, particularly in hot climates. In the present study, stability of the five, most-used NDV vaccine strains (I-2, LaSota, B1, Clone 30 [C30], and VG-GA) was tested comparatively at different storage temperatures (4 and 37 C for the freeze-dried form and 4, 24, 37, and 45 C for the freeze-dried vaccine reconstituted in diluents). The vaccine stability was evaluated by the cumulative infectious titer drop and the theoretical shelf life at particular temperatures. Results showed that I-2 and LaSota are the most stable vaccine strains compared to B1, C30, and VG-GA; they registered the lowest titer drops and the longest shelf life whether at cool, high, or room temperatures and for both freeze-dried and reconstituted vaccines.

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Long-term stability studies on protection against Newcastle disease by commercial live vaccine used in Brazil

Cited by 3 publications

References 13 publications

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Suitability of differently formulated dry powder Newcastle disease vaccines for mass vaccination of poultry

Electrochemical behavior of Newcastle virus La Sota strain (Newvac vaccine) in the presence of gold nanoparticles as sensitizers investigated on Au electrode

Thermal Stability Study of Five Newcastle Disease Attenuated Vaccine Strains

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